Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural
Grantová podpora
R03 DE027146
NIDCR NIH HHS - United States
- Klíčová slova
- Conditioned medium, Exosomes, Mesenchymal stem cells, Ultracentrifugation, miRNAs,
- MeSH
- exozómy * genetika MeSH
- kmenové buňky MeSH
- lidé MeSH
- mezenchymální kmenové buňky * MeSH
- mikro RNA * genetika MeSH
- ultracentrifugace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- mikro RNA * MeSH
Exosomes, derived from stem cells, have great promise in regenerative medicine due to their capabilities of ameliorating inflammation, preventing tissue damage and promoting healing, which in part are associated with the exosomal RNA/miRNA. The application of mesenchymal stem cell exosomes in treating hepatic disorders including nonalcoholic fatty liver disease has drawn much attention. In this chapter, we describe our experience in culturing human mesenchymal stem cells and isolating their exosomes from culture medium through ultracentrifugation. Methods to extract exosomal RNA/miRNA are also discussed.
Zobrazit více v PubMed
Baglio SR, Pegtel DM, Baldini N (2012) Mesenchymal stem cell secreted vesicles provide novel opportunities in (stem) cell-free therapy. Front Physiol 3:359 DOI
Maguire G (2013) Stem cell therapy without the cells. Commun Integr Biol 6(6):e26631 DOI
Ranganath SH, Levy O, Inamdar MS et al (2012) Harnessing the mesenchymal stem cell secretome for the treatment of cardiovascular disease. Cell Stem Cell 10(3):244–258 DOI
Kalluri R, LeBleu VS (2020) The biology, function, and biomedical applications of exosomes. Science 367(6478):eaau6977 DOI
Tkach M, Thery C (2016) Communication by extracellular vesicles: where we are and where we need to go. Cell 164(6):1226–1232 DOI
Emanueli C, Shearn AI, Angelini GD, Sahoo S (2015) Exosomes and exosomal miRNAs in cardiovascular protection and repair. Vascul Pharmacol 71:24–30 DOI
Ibrahim AG, Cheng K, Marban E (2014) Exosomes as critical agents of cardiac regeneration triggered by cell therapy. Stem Cell Rep 2(5):606–619 DOI
Lai RC, Arslan F, Lee MM et al (2010) Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Res 4(3):214–222 DOI
Tan CY, Lai RC, Wong W et al (2014) Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models. Stem Cell Res Ther 5(3):76 DOI
Zhao H, Shang Q, Pan Z et al (2018) Exosomes from adipose-derived stem cells attenuate adipose inflammation and obesity through polarizing M2 macrophages and beiging in white adipose tissue. Diabetes 67(2):235–247 DOI
Zhang S, Hou Y, Yang J et al (2020) Application of mesenchymal stem cell exosomes and their drug-loading systems in acute liver failure. J Cell Mol Med 24(13):7082–7093 DOI
Liu Y, Lou G, Li A et al (2018) AMSC-derived exosomes alleviate lipopolysaccharide/d-galactosamine-induced acute liver failure by miR-17-mediated reduction of TXNIP/NLRP3 inflammasome activation in macrophages. EBioMedicine 36:140–150 DOI
Lou G, Yang Y, Liu F et al (2017) MiR-122 modification enhances the therapeutic efficacy of adipose tissue-derived mesenchymal stem cells against liver fibrosis. J Cell Mol Med 21(11):2963–2973 DOI
Qu Y, Zhang Q, Cai X et al (2017) Exosomes derived from miR-181-5p-modified adipose-derived mesenchymal stem cells prevent liver fibrosis via autophagy activation. J Cell Mol Med 21(10):2491–2502 DOI
Thery C, Witwer KW, Aikawa E et al (2018) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 7(1):1535750 DOI
