The sucrose synthase (SS) is an important enzyme family which play a vital role in sugar metabolism to improve the fruit quality of the plants. In many plant species, the members of SS family have been investigated but the detailed information is not available in legumes particularly and Glycine max specifically. In the present study, we found thirteen SS members (GmSS1-GmSS13) in G. max genome. High conserved regions were present in the GmSS sequences that may due to the selection pressure during evolutionary events. The segmental duplication was the major factor to increase the number of GmSS family members. The identified thirteen GmSS genes were divided into Class I, Class II and Class III with variable numbers of genes in each class. The protein interaction of GmSS gave the co-expression of sucrose synthase with glucose-1-phosphate adenylyltransferase while SLAC and REL test found number of positive sites in the coding sequences of SS family members. All the GmSS family members except GmSS7 and few of class III members, were highly expressed in all the soybean tissues. The expression of the class I members decreased during seed development, whireas, the class II members expression increased during the seed developing, may involve in sugar metabolism during seed development. Solexa sequencing libraries of acidic condition (pH 4.2) stress samples showed that the expression of class I GmSS genes increased 1- to 2-folds in treated samples than control. The differential expression pattern was observed between the members of a paralogous. This study provides detailed genome-wide analysis of GmSS family in soybean that will provide new insights for future evolutionary and soybean breeding to improve the plant growth and development.

Department of Agrochemistry Soil Science Microbiology and Plant Nutrition Faculty of AgriSciences Mendel University in Brno Brno Czech Republic

Department of Animal Breeding and Genetics University of Veterinary and Animal Sciences Lahore Pakistan

Department of Biochemistry Shaheed Benazir Bhutto Women University Peshawar Pakistan

Department of Biotechnology University of Science and Technology Bannu Pakistan

Department of Botany and Microbiology College of Science King Saud University Riyadh Saudi Arabia

Department of Plant Breeding and Genetics Gomal University D 1 Khan Pakistan

Department of Plant Breeding and Genetics University of Agriculture D 1 Khan Pakistan

Department of Statistics Shaheed Benazir Bhutto Women University Peshawar Pakistan

Institute de Farmacia Facultad de Ciencias Universidad Austral de Chile Campus Isla Teja Valdivia Chile

Pedologiejh spol s r o Brno Czech Republic

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PLoS One. 2022 Sep 14;17(9):e0274409. doi: 10.1371/journal.pone.0274409. PubMed

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