Cold and nutrient-activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine-tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on Gm15551, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β-adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.

Cluster of Excellence Cellular Stress Responses in Aging Associated Diseases Faculty of Medicine University Hospital of Cologne University of Cologne Joseph Stelzmann Str 26 50931 Cologne Germany

Czech Centre for Phenogenomics Institute of Molecular Genetics of the Czech Academy of Sciences Prumyslova 595 25250 Vestec Czech Republic

Department for Biochemistry and Molecular Biology University of Southern Denmark Campusvej 55 5230 Odense Denmark

Institute for Diabetes and Cancer Helmholtz Zentrum München German Research Center for Environmental Health 85764 Neuherberg Germany

Laboratory of Animal Physiology Department of Animal Science and Technology National Taiwan University Taipei 10617 Taiwan

Max Planck Institute for Metabolism Research Gleueler Strasse 50 50931 Köln Germany

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