Stable inter-individual differences in behaviour and personality have been studied for several decades now. The aim of this study was to test the repeatability of behaviour of the Long Evans strain of laboratory rats in order to assess their inter-individual differences. Male laboratory rats (n = 36) were tested in a series of tasks (Open field test, Elevated plus maze test, and modified T-maze test) repeated over time to assess their personality traits. To evaluate the temporal stability of the behaviour, we calculated repeatability estimates of the examined traits. We also checked for a link in behavioural traits across these experiments, which would suggest the existence of a behavioural syndrome. We found stable inter-individual differences in behaviour. Interestingly, no link emerged between the tasks we studied and therefore we did not find support for a behavioural syndrome. The lack of behavioural correlations between these experiments suggests that the results derived from these tasks should be interpreted carefully, as these experiments may measure various behavioural axes. Moreover, the animals habituate to the apparatus. Consequently, behaviour in the Open field test and Elevated plus maze test is not fully consistent and repeatable across subsequent trials.

Department of Ethology and Companion Animal Science Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague Czech Republic

Department of Physiology Faculty of Science Charles University Prague Czech Republic

Department of Zoology Faculty of Science Charles University Prague Czech Republic

Institute of Physiology Czech Academy of Sciences Prague Czech Republic

National Institute of Mental Health Klecany Czech Republic

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