While nostocacean cyanobacteria are ubiquitous and play critical roles in terrestrial ecosystems, their taxonomy and biogeography still entail mysteries. We isolated two Nostoc-like cyanobacteria from biological soil crusts of the Atacama (Chile) and Mojave (USA) Deserts. An initial 16S rRNA gene phylogeny placed both in monophyly with Mojavia pulchra. Here, we describe two new species of the previously monotypic Mojavia using a polyphasic approach including morphology, 16S rRNA phylogenies, secondary structure, and percent similarity of the 16S-23S ITS region. Like M. pulchra, both new species produce compact microcolonies, arthrospore-like akinetes, and monocytes, traits characteristic of the genus. Mojavia aguilerae sp. nov. is morphologically distinct from both other species in producing bluntly conical end cells, abundant enlarged akinetes in multiseriate filaments, and gold-colored cells during senescence. Mojavia dolomitestris sp. nov. exhibited distinctly firm, light-colored, compartmentalized mucilage. M. dolomitestris is somewhat cryptic with M. pulchra, but has more densely packed microcolonies, rarity and later onset of brownish sheath pigmentation, and an origin from soils derived from dolomite. The two new species strengthened the position of Mojavia as a robust genus sister to Nostoc. Although 16S rRNA gene data could not separate the Mojavia species from each other, the three species showed distinct dissimilarities in secondary ITS structure and differed greatly from Nostoc sensu stricto. The high dissimilarities between their 16S-23S ITS regions suggest a long evolutionary history of the three species as separate lineages. Mojavia is an evolutionary and ecologically unique nostocacean genus, and its rarity and restricted habitat point to an urgent need for recognition and protection.

Departamento de Biología Comparada Universidad Nacional Autónoma de México Colonia Coyoacán Código Postal 04451070474 P O Box 70 474 Ciudad de México Mexico

Department of Biological Sciences Kent State University Kent Ohio 44242 USA

Department of Biology John Carroll University University Heights Ohio 44118 USA

Department of Botany Faculty of Sciences University of South Bohemia Branišovská 31 České Budějovice 370 05 Czech Republic

Plant and Environmental Sciences Department New Mexico State University 945 College Drive Las Cruces New Mexico 88003 USA

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Alwathnani, H. & Johansen, J. R. 2011. Cyanobacteria in soils from a Mojave Desert ecosystem. Monogr. Western North Am. Nat. 5:71-89.

Alvarenga, L. V. d., Vieira Vaz, M. G. M., Genuário, D. B., Esteves-Ferreira, A. A., Almeida, A. V. M., de Castro, N. V., Lizieri, C., Souza, J. J. L. L., Schaefer, C. E. G. R., Nunes-Nesi, A. & Araújo, W. L. 2018. Extending the ecological distribution of Desmonostoc genus: proposal of Desmonostoc salinum sp. nov., a novel Cyanobacteria from a saline-alkaline lake. Int. J. Syst. Evol. Microbiol. 68:2770-82.

Anderson, D. C. & Rushforth, S. R. 1976. The cryptogamic flora of desert soil crusts in southern Utah. Nova Hedwigia 28:691-729.

Bagchi, S. N., Dubey, N. & Singh, P. 2017. Phylogenetically distant clade of Nostoc-like taxa with the description of Aliinostoc gen. Nov. and Aliinostoc morphoplasticum sp. nov. Int. J. Syst. Evol. Microbiol. 67:3329-38.

Bahl, J., Lau, M. C. Y., Smith, G. J. D., Vijaykrishna, D., Cary, S. C., Lacap, D. C., Lee, C. K. et al. 2011. Ancient origins determine global biogeography of hot and cold desert cyanobacteria. Nat. Commun. 2:163.

Becerra-Absalón, I., Johansen, J. R., Muñoz-Martin, M. A. & Montejano, G. 2018. Chroakolemma gen. Nov. (Leptolyngbyaceae, Cyanobacteria) from soil biocrusts in the semi-desert Central Region of Mexico. Phytotaxa 367:201-18.

Becerra-Absalón, I., Johansen, J. R., Osorio-Santos, K. & Montejano, G. 2020. Two new Oculatella (Oculatellaceae, Cyanobacteria) species in soil crusts from tropical semi-arid uplands of México. Fottea 20:160-70.

Bohunická, M., Pietrasiak, N., Johansen, J. R., Berrendero-Gomez, E., Hauer, T., Gaysina, L. & Lukešová, A. 2015. Roholtiella, gen. Nov. (Nostocales, Cyanobacteria) - a tapering and branching member of the Nostocaceae (Cyanobacteria). Phytotaxa 197:84-103.

Boyer, S. L., Johansen, J. R., Flechtner, V. R. & Howard, G. L. 2002. Phylogeny and genetic variance in terrestrial Microcoleus (Cyanophyceae) species based on sequence analysis of the 16S rRNA gene and associated 16S-23S ITS region. J. Phycol. 38:1222-35.

Brown, A. O., Romanis, C. S., Dvořák, P., Foss, A. J., Gibson, Q. A., Villanueva, C. D., Durden, W. N. et al. 2021. A new species of cryptic cyanobacteria isolated from the epidermis of a bottlenose dolphin and as a bioaerosol. Phycologia 60:603-18.

Cai, F. & Li, R. H. 2019. Validation of Compactonostoc shennongjiaense gen. et sp. nov. (Nostocaceae, Cyanobacteria). Not. Alg. 121:1.

Cai, F. & Li, R. 2020. Purpureonostoc, a new name for a recently described genus of Nostoc-like cyanobacteria. Fottea 20:111.

Cai, F., Li, X., Yang, Y., Jia, N., Huo, D. & Li, R. 2019a. Compactonostoc shennongjiaensis gen. & sp. nov. (Nostocales, Cyanobacteria) from a wet rocky wall in China. Phycologia 58:200-10.

Cai, F., Li, X., Geng, R., Peng, X. & Li, R. 2019b. Phylogenetically distant clade of Nostoc-like taxa with the description of Minunostoc gen. Nov. and Minunostoc cylindricum sp. nov. Fottea 19:13-24.

Cai, F., Peng, X. & Li, R. 2020a. Violetonostoc minutum gen et sp. nov. (Nostocales, Cyanobacteria) from a rocky substrate in China. Algae. Int. J. Algal Res. 35:1-15.

Cai, F., Wang, Y., Yu, G., Wang, J., Peng, X. & Li, R. 2020b. Proposal of Purpurea gen. Nov. (Nostocales, Cyanobacteria), a novel cyanobacterial genus from wet soil samples in Tibet. China. Fottea 20:86-97.

Cai, F., Yu, G., Liu, Y., Sun, Y. & Li, R. 2021. Description of two new species of Nostoc from China based on the polyphasic approach. Fottea 21:259-71.

Cameron, R. E. 1960. Communities of soil algae occurring in the Sonoran Desert of Arizona. J. Ariz. Acad. Sci. 1:85-8.

Carmichael, W. W. 1986. Isolation, culture, and toxicity testing of toxic freshwater cyanobacteria (blue-green algae). In Shilov, V. [Ed.] Fundamental Research in Homogenous Catalysis. Gordon & Breach Publ., New York, Vol. 3, pp. 1249-62.

Casamatta, D. A., Gomez, S. R. & Johansen, J. R. 2006. Rexia erecta gen. et sp. nov. and Capsosira lowei sp. nov., two newly described cyanobacterial taxa from the Great Smoky Mountain National Park (USA). Hydrobiologia 561:13-26.

Drummond, A. J., Ho, S. Y. W., Philips, M. J. & Rambaut, A. 2006. Relaxed phylogenetics and dating with confidence. PLoS Biol. 4:e88.

Erwin, P. M. & Thacker, R. W. 2008. Cryptic diversity of the symbiotic cyanobacterium Synechococcus spongiarum among sponge host. Mol. Ecol. 17:2937-47.

Evans, R. D. & Johansen, J. R. 1999. Microbiotic crusts and ecosystem processes. Critical Rev. Plant Sci. 18:183-225.

Flechtner, V. R., Boyer, S. L., Johansen, J. R. & DeNoble, M. L. 2002. Spirirestis rafaelensis gen. et sp. nov. (Cyanophyceae), a new cyanobacterial genus from arid soils. Nova Hedwigia 74:1-24.

Flechtner, V. R., Johansen, J. R. & Belnap, J. 2008. The biological soil crusts of the San Nicolas Island: enigmatic algae from a geographically isolated ecosystem. W. North Amer. Nat. 68:405-36.

Forest, H. S. & Weston, C. R. 1966. Blue-green algae from the Atacama Desert of northern Chile. J. Phycol. 2:163-4.

Garcia-Pichel, F., Prufert-Bebout, L. & Muyzer, G. 1996. Phenotypic and phylogenetic analyses show Microcoleus chthonoplastes to be a cosmopolitan cyanobacterium. Appl. Environ. Microbiol. 62:3284-91.

Gelman, A. & Rubin, D. B. 1992. Inference from iterative simulation using multiple sequences. Stat. Sci. 7:157-511.

Genuário, D. B., Vieira Vaz, M. G. M., Hentschke, G. S., Sant'Anna, C. L. & Fiore, M. F. 2015. Halotia gen. Nov., a phylogenetically and physiologically coherent cyanobacterial genus isolated from marine coastal environments. Int. J. Syst. Evol. Microbiol. 65:663-75.

González-Resendiz, L., Johansen, J. R., Escobar-Sánchez, V., Segal-Kischinevzky, C., Jiménez-García, L. F. & León-Tejera, H. 2018a. Two new species of Phyllonema (Rivulariaceae, Cyanobacteria) with an emendation of the genus. J. Phycol. 54:638-52.

González-Resendiz, L., Johansen, J. R., Alba-Lois, L., Segal-Kischinevzky, C., Escobar-Sánchez, V., Jimenez Garcia, L. F., Hauer, T. & León-Tejera, H. 2018b. Nunduva, a new marine genus of Rivulariaceae (Nostocales, Cyanobacteria) from marine tropical rocky shores. Fottea 18:86-105.

Hauerová, R., Hauer, T., Kaštovský, J., Komárek, J., Lepšová-Skácelová, O. & Mareš, J. 2021. Tenebriella gen.nov. - the dark twin of Oscillatoria. Mol. Phylog. Evol. 165:107293.

Hentschke, G. S., Johansen, J. R., Pietrasiak, N., Rigonato, J., Fiore, M. F. & Sant'Anna, C. L. 2017. Komarekiella atlantica gen. et sp. nov. (Nostocaceae, Cyanobacteria): a new subaerial taxon from the Atlantic Rainforest and Kauai, Hawaii. Fottea 17:178-90.

Higgins, D. G. & Sharp, P. M. 1988. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237-44.

Hrouzek, P., Lukešová, A., Mareš, J. & Ventura, S. 2013. Description of the cyanobacterial genus Desmonostoc gen. Nov. including D. muscorum comb. nov. as a distinct, phylogenetically coherent taxon related to the genus Nostoc. Fottea 13:201-13.

Ionescu, D., Hindiyeh, M., Malkawi, H. & Oren, A. 2010. Biogeography of thermophilic cyanobacteria: insights from the Zerka Ma'in hot springs (Jordan). FEMS Microbiol. Ecol. 72:103-13.

Iteman, I., Rippka, R., Tadeuau de Marsac, N. & Herdman, M. 2000. Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria. Microbiology 146:1275-86.

Johansen, J. R. 1993. Minireview: cryptogamic crusts of semiarid and arid lands of North America. J. Phycol. 29:139-47.

Johansen, J. R. & Casamatta, D. A. 2005. Recognizing cyanobacterial diversity through adoption of a new species paradigm. Algol. Stud. 116:71-93.

Johansen, J. R., Mareš, J., Pietrasiak, N., Bohunická, M., Zima, J., Štenclová, L. & Hauer, T. 2017. Highly divergent 16S rRNA sequences in ribosomal operons of Scytonema hyalinum (Cyanobacteria). PLoS ONE 12:e0186393.

Jung, P., Mikhailyuk, T., Emrich, D., Baumann, K., Dultz, S. & Büdel, B. 2020. Shifting boundaries: ecological and geographical range extension based on three new species in the cyanobacterial genera Cyanocohniella, Oculatella, and Aliterella. J. Phycol. 56:1216-31.

Kabirnataj, S., Nematzadeh, G. A., Talebi, A. F., Saraf, A., Suradkar, A., Tabatabaei, M. & Singh, P. 2020. Description of novel species of Allinostoc, Desikacharya and Desmonostoc using a polyphasic approach. Int. J. Syst. Evol. Microbiol. 70:3413-26.

Komárek, J. 2013. Cyanoprokaryota 3. Teil/3rd Part: Heterocytous genera. In Büdel, B., Gärtner, G., Krienitz, L. & Schagerl, M. [Eds.] Süßwasserflora von Mitteleuropa 19/3. Springer Spektrum, Berlin, Heidelberg, p. 1130.

Komárek, J., Kaštovsky, J., Mareš, J. & Johansen, J. R. 2014. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014 according to the polyphasic approach. Preslia 86:295-335.

Kurmayer, R. & Gumpenberger, M. 2006. Diversity of microcystin genotypes among populations of the filamentous cyanobacteria Planktothrix rubescens and Planktothrix agardhii. Mol. Ecol. 15:3849-61.

Mai, T., Johansen, J. R., Pietrasiak, N., Bohunická, M. & Martin, M. P. 2018. Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. Nov. and Trichocoleaceae fam. Nov. and six new genera containing 14 species. Phytotaxa 325:1-59.

Mareš, J., Johansen, J. R., Hauer, T., Zima, J., Jr., Ventura, S., Cuzman, O., Tiribilli, B. & Kaštovský, J. 2019. Taxonomic resolution of the genus Cyanothece (Chroococcales, Cyanobacteria), with a treatment on Gloeothece and three new genera, Crocosphaera, Rippkaea, and Zehria. J. Phycol. 55:578-610.

Martiny, A. C., Tai, A. P. K., Veneziano, D., Primeau, F. & Chisholm, S. W. 2009. Taxonomic resolution, ecotypes and the biogeography of Prochlorococcus. Env. Microbiol. 11:823-32.

Metting, B. 1981. The systematics and ecology of soil algae. Bot. Rev. 47:195-312.

Mikhailyuk, T., Vinogradova, O., Holzinger, A., Glaser, K., Akimov, Y. & Karsten, U. 2022. Timaviella dunensis sp. nov. from sand dunes of the Baltic Sea, Germany, and emendation of Timaviella edaphica (Elenkin) O.M. Vynogr. & Mikhailyuk (Synechococcales, Cyanobacteria) based on an integrative approach. Phytotaxa 532:192-208.

Miller, M., Schwartz, T., Pickett, B., He, S., Klem, E., Scheuermann, R. H., Passarotti, M., Kaufman, S. & O'Leary, M. A. 2015. A RESTful API for Access to Phylogenetic Tools via the CIPRES Science Gateway. Evol. Bioinform. 11:43-8.

Miller, S. R., Castenholz, R. W. & Pedersen, D. 2007. Phylogeography of the thermophilic cyanobacterium Mastigocladus laminosus. Appl. Environ. Microbiol. 73:4751-9.

Miscoe, L. H., Johansen, J. R., Vaccarino, M. A., Pietrasiak, N. & Sherwood, A. R. 2016. Novel cyanobacteria from caves on Kauai, Hawaii. Bibliotheca Phycologica 120:75-152.

Moreira, C., Fathalli, A., Vasconcelos, V. & Antunes, A. 2011. Genetic diversity and structure of the invasive toxic cyanobacterium Cylindrospermopsis raciborskii. Curr. Microbiol. 62:1590-5.

Moreira, C., Vasconcelos, V. & Antunes, A. 2013. Phylogeny and biogeography of cyanobacteria and their produced toxins. Mar. Drugs 11:4350-69.

Mühlsteinová, R., Johansen, J. R., Pietrasiak, N., Martin, M. P., Osorio-Santos, K. & Warren, S. D. 2014a. Polyphasic characterization of Trichocoleus desertorum sp. nov. (Pseudanabaenales, Cyanobacteria) from desert soils and phylogenetic placement of the genus Trichocoleus. Phytotaxa 193:241-61.

Mühlsteinová, R., Johansen, J. R., Pietrasiak, N. & Martin, M. P. 2014b. Polyphasic characterization of Kastovskya adunca gen. Nov. et comb. nov. (Oscillatoriales, Cyanobacteria) from desert soils of the Atacama Desert, Chile. Phytotaxa 163:216-28.

Novichkova-Ivanova, L. N. 1980. Soil Algae of the Sahara-Gobi Desert Region. Nauka. Leningrad 256 pp. (in Russian).

Osorio-Santos, K., Pietrasiak, N., Bohunická, M., Miscoe, L. H., Kováčik, L., Martin, M. P. & Johansen, J. R. 2014. Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria). Eur. J. Phycol. 49:450-70.

Patzelt, D. J., Hodač, L., Friedl, T., Pietrasiak, N. & Johansen, J. R. 2014. Biodiversity of soil cyanobacteria in the hyper-arid Atacama Desert, Chile. J. Phycol. 50:698-710.

Pereira, I., Razmilic, I. & Johansen, J. R. 2014. Content of phycobilin pigments in two strains of cyanobacteria of the Atacama Desert, Chile. SOJ Microbiol. Infect. Dis. 2:1-3.

Perkerson, R., Johansen, J. R., Kovacik, L., Brand, J. & Casamatta, D. A. 2011. Pseudanabanalean (Cyanobacteria) genus Nodosilinea gen. Nov. based on morphological and molecular data. J. Phycol. 47:1397-412.

Pietrasiak, N. 2012. Effects of Land Surface Characteristics on Pedogenesis, Biological Soil Crust Community Diversity, and Ecosystem Functions in a Mojave Desert Piedmont Landscape. Ph.D. dissertation. University of California, Riverside, California, 225 p.

Pietrasiak, N., Mühlsteinová, R., Siegesmund, M. & Johansen, J. R. 2014a. Phylogenetic placement of Symplocastrum (Phormidiaceae, Cyanobacteria) with descriptions of two new species: S. flechtnerae and S. torsivum. Phycologia 53:529-41.

Pietrasiak, N., Drenovsky, R. E., Santiago, L. S. & Graham, R. C. 2014b. Biogeomorphology of a Mojave Desert Landscape - configurations and feedbacks of abiotic and biotic land surfaces during landform evolution. Geomorphology 206:23-36.

Pietrasiak, N., Osorio-Santos, K., Shalygin, S., Martin, M. P. & Johansen, J. R. 2019. When is a lineage a species? A case study in Myxacorys gen. Nov. (Synechococcales: Cyanobacteria) with the description of two new species from the Americas. J. Phycol. 55:976-96.

Pietrasiak, N., Osorio-Santos, K., Lipson, D. L. & Johansen, J. R. 2021. Trichotorquatus gen. Nov. - a new genus of soil cyanobacteria discovered from American drylands. J. Phycol. 57:886-902.

Rajaniemi, P., Komárek, J., Willame, R., Hrouzek, P., Kastovská, K., Hoffmann, L. & Sivonen, K. 2005. Taxonomic consequences from the combined molecular and phenotype evaluation of selected Anabaena and Aphanizomenon strains. Algol. Stud. 117:371-91.

Řeháková, K., Johansen, J. R., Casamatta, D. A., Xuesong, L. & Vincent, J. 2007. Morphological and molecular characterization of selected desert soil cyanobacteria: three species new to science including Mojavia pulchra gen. et sp. nov. Phycologia 46:481-502.

Řeháková, K., Johansen, J. R., Bowen, M. B., Martin, M. P. & Sheil, C. A. 2014. Variation in secondary structure of the 16S rRNA molecule in cyanobacteria with implications for phylogenetic analysis. Fottea 14:161-78.

Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D. L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. A. & Huelsenbeck, J. P. 2012. MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61:539-42.

Saraf, A., Dawda, H. G. & Singh, P. 2019a. Desikacharya gen. Nov., a phylogenetically distinct genus of Cyanobacteria along with the description of two new species, Desikacharya nostocoides sp. nov., and Desikacharya soli sp. nov., and reclassification of Nostoc thermotolerans to Desikacharya thermotolerans comb. nov. Int. J. Syst. Evol. Microbiol. 69:307-15.

Saraf, A. G., Dawda, H. G. & Singh, P. 2019b. Validation of the genus Desikacharya gen. Nov. (Nostocaceae, Cyanobacteria) and three included species. Not. Alg. 107:1-3.

Schwabe, G. H. 1960. Zur autotrophen Vegetation in ariden Böden. Blaualgen und Lebensraum IV. Österr. Bot. Zeit. 107:281-309.

Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-3.

Thiers, B.M. 2022 [continuously updated]. Index Herbariorum: a Global Directory of Public Herbaria and Associated Staff. New York Botanical Garden's Virtual Herbarium. http://sweetgum.nybg.org/science/ih/

Vaccarino, M. A. & Johansen, J. R. 2011. Scytonematopsis contorta sp. nov. (Nostocales), a new species from the Hawaiian Islands. Fottea 11:149-61.

Van Gremberghe, I., Leliaert, F., Mergeay, J., Vanormelingen, P., Van der Gucht, K., Debeer, A.-E., Lacerot, G., De Meester, L. & Vyverman, W. 2011. Lack of phylogeographic structure in the freshwater cyanobacterium Microcystis aeruginosa suggests global dispersal. PLoS ONE 6:e19561.

Vázquez-Martínez, J., Gutierrez-Villagomez, J. M., Fonesca-García, C., Ramírez-Chávez, E., Mondragón-Sánchez, M. L., Partida-Martínez, L., Johansen, J. R. & Molina-Torres, J. 2018. Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico. Phytotaxa 334:167-82.

Ward, R. D., Stajich, J. E., Johansen, J. R., Huntemann, M., Clum, A., Foster, B., Foster, B. et al. 2021. Metagenome sequencing to explore phylogenomics of terrestrial cyanobacteria. Microbiol. Resour. Announc. 10:e00258-21.

Zuker, M. 2003. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31:3406-15.

Revision and expansion of the genus Spirirestis (Tolypothrichaceae, Cyanobacteria)