Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
35765178
PubMed Central
PMC9108439
DOI
10.1002/mbo3.1286
Knihovny.cz E-zdroje
- Klíčová slova
- Bionectriaceae, beetle-fungus symbiosis, cross-amplification, detection, microsatellite markers,
- MeSH
- brouci * mikrobiologie MeSH
- Hypocreales * genetika MeSH
- Juglans * mikrobiologie MeSH
- mikrosatelitní repetice genetika MeSH
- nemoci rostlin * mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Tennessee MeSH
Symbioses between Geosmithia fungi and wood-boring and bark beetles seldom result in disease induction within the plant host. Yet, exceptions exist such as Geosmithia morbida, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts, and Geosmithia sp. 41, the causal agent of Foamy Bark Canker disease of oaks. Isolates of G. obscura were recovered from black walnut trees in eastern Tennessee and at least one isolate induced cankers following artificial inoculation. Due to the putative pathogenicity and lack of recovery of G. obscura from natural lesions, a molecular diagnostic screening tool was developed using microsatellite markers mined from the G. obscura genome. A total of 3256 candidate microsatellite markers were identified (2236, 789, 137 di-, tri-, and tetranucleotide motifs, respectively), with 2011, 703, 101 di-, tri-, and tetranucleotide motifs, respectively, containing markers with primers. From these, 75 microsatellite markers were randomly selected, screened, and optimized, resulting in 28 polymorphic markers that yielded single, consistently recovered bands, which were used in downstream analyses. Five of these microsatellite markers were found to be specific to G. obscura and did not cross-amplify into other, closely related species. Although the remaining tested markers could be useful, they cross-amplified within different Geosmithia species, making them not reliable for G. obscura detection. Five novel microsatellite markers (GOBS9, GOBS10, GOBS41, GOBS43, and GOBS50) were developed based on the G. obscura genome. These species-specific microsatellite markers are available as a tool for use in molecular diagnostics and can assist future surveillance studies.
Department of Entomology and Plant Pathology The University of Tennessee Knoxville Tennessee USA
Department of Plant Pathology University of Florida Homestead Florida USA
Department of Plant Sciences The University of Tennessee Knoxville Tennessee USA
Institute of Microbiology Czech Academy of Sciences Prague Czech Republic
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