Due to translocation heterozygosity for all chromosomes in the cell complement, the oyster plant (Tradescantia spathacea) forms a complete meiotic ring. It also shows Rabl-arrangement at interphase, featured by polar centromere clustering. We demonstrate that the pericentromeric regions of the oyster plant are homogenized in concert by three subtelomeric sequences: 45S rDNA, (TTTAGGG)n motif, and TSrepI repeat. The Rabl-based clustering of pericentromeric regions may have been an excellent device to combine the subtelomere-pericentromere sequence migration (via inversions) with the pericentromere-pericentromere DNA movement (via whole arm translocations) that altogether led to the concerted homogenization of all the pericentromeric domains by the subtelomeric sequences. We also show that the repetitive sequence landscape of interstitial chromosome regions contains many loci consisting of Arabidopsis-type telomeric sequence or of TSrepI repeat, and it is extensively heterozygous. However, the sequence arrangement on some chromosomal arms suggest segmental inversions that are fully or partially homozygous, a fact that could be explained if the inversions started to create linkages already in a bivalent-forming ancestor. Remarkably, the subterminal TSrepI loci reside exclusively on the longer arms that could be due to sharing sequences between similarly-sized chromosomal arms in the interphase nucleus. Altogether, our study spotlights the supergene system of the oyster plant as an excellent model to link complex chromosome rearrangements, evolution of repetitive sequences, and nuclear architecture.

Department of Biomedicine and Biotechnology University of Alcalá 28871 Alcalá de Henares Madrid Spain

Department of Molecular Biology Institute of Biological Sciences John Paul 2 Catholic University of Lublin Konstantynów 1i 20 708 Lublin Poland

Department of Plant Biology and Biotechnology University of Agriculture in Krakow Al 29 Listopada 54 31 425 Cracow Poland

Institute of Experimental Botany of the Czech Academy of Sciences Centre of the Region Hanaá for Biotechnological and Agricultural Research Šlechtitelů 31 77900 Olomouc Czech Republic

Max Planck Institute of Molecular Plant Physiology Am Mühlenberg 1 14476 Potsdam Golm Germany

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