Separation of triacylglycerols containing positional isomers of hexadecenoic acids by enantiomeric liquid chromatography-mass spectrometry
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
35921696
DOI
10.1016/j.jchromb.2022.123401
PII: S1570-0232(22)00305-1
Knihovny.cz E-resources
- Keywords
- Cis-palmitvaccenic, Enantiomeric liquid chromatography-mass spectrometry, Hypogeic acids, Palmitoleic,
- MeSH
- Chromatography, Liquid MeSH
- Mass Spectrometry methods MeSH
- Palmitic Acids * MeSH
- Fatty Acids * analysis MeSH
- Triglycerides analysis MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- hexadecenoic acid MeSH Browser
- Palmitic Acids * MeSH
- Fatty Acids * MeSH
- Triglycerides MeSH
Triacylglycerols (TAGs) containing positional isomers of hypogeic (Hy), palmitoleic (Po), and palmitvaccenic (Pv) acids from three microorganisms (top-fermenting brewer's yeast Saccharomyces cerevisiae, green alga Coccomyxa elongata, and arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis) were analyzed. Dozens of regioisomers and enantiomers of TAGs containing one, two or three hexadecenoic acids have been identified by means of reversed phase chromatography/mass spectrometry (RP-HPLC/MS). The regioisomers of TAGs containing two palmitic acids and any hexadecenoic acid were separated. Analysis of regioisomers of TAGs having one Pv residue showed that asymmetric molecular species such as PvPP or PPPv were dominant in Rhizophagus. TAGs were also analyzed on a chiral phase column and nine molecular species of TAGs containing two palmitic and any of three hexadecenoic acids were separated and identified. In the case of TAGs containing one palmitic and two hexadecenoic acids, the separation was successful only if the hexadecenoic acids were identical. Separation of TAGs containing three hexadecenoic acids was successful only if all three hexadecenoic acids were identical. Regardless of the type of TAG, it was found that TAGs in the AM fungus and containing palmitvaccenic acid bound at the sn-1 position of the glycerol backbone were dominant, suggesting similarity in the biosynthesis of the different TAGs. The covalent adduct chemical ionization method was used for identification of TAGs as adduct with (1-methyleneimino)-1-ethenyl ion, which reacted with double bond of the unsaturated fatty acid. Tandem MS thus makes it possible to identify TAGs containing various hexadecenoic acids.
Department of Ecology Faculty of Science Charles University Viničná 7 128 44 Prague 2 Czech Republic
Institute of Botany Czech Academy of Sciences Dukelská 135 379 82 Třeboň Czech Republic
Institute of Microbiology Czech Academy of Sciences Vídeňská 1083 142 20 Prague 4 Czech Republic
References provided by Crossref.org
Nutrient-dependent cross-kingdom interactions in the hyphosphere of an arbuscular mycorrhizal fungus
