In Poland, distribution of non-native pumpkinseed Lepomis gibbosus (Centrarchidae) is strictly limited to the Oder river basin, where it was introduced in the early 20th century. Recently, several populations have been found in waterbodies adjacent to the Oder, particularly in its lower reaches. In this study, we compare the genetic relatedness of populations in the Oder basin with other European populations using nuclear (microsatellite) and mitochondrial (partial cytochrome c oxidase subunit I; cox1) markers. Microsatellite analysis indicated that four populations in the lower Oder form a separate cluster, while one in the middle Oder clustered with Danubian populations, from where probably having been introduced. Microsatellite data suggested that the lower Oder populations differ from other non-native European populations, making it impossible to estimate the source of introduction. Nevertheless, analysis of cox1 indicated that Oder pumpkinseeds belong to the same haplotype as the vast majority of European populations. Parasitological examination confirmed the presence of two North American species, the monogenean Onchocleidus dispar and trematode Posthodiplostomum centrarchi, in the lower Oder, both previously unknown in the region. Fifteen other parasite species were acquired, including glochidia of invasive Sinanodonta woodiana. In the middle Oder, parasite infection was more limited. Fish from the Gryfino Canal, considered one of the most invasive populations in Europe, showed the highest parasite abundance and diversity, and the highest somatic condition and growth rate due to warm water released from the Dolna Odra power plant. Our results highlight significant differences in somatic condition and parasite infection in long-established non-native pumpkinseed populations in the same river system, reflecting mainly environmental conditions.

Department of Ecology and Vertebrate Zoology University of Łódź Łódź Poland

Institute of Marine Biology National Academy of Science of Ukraine Odessa Ukraine

Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic

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Ağdamar, S., Tarkan, A. S., Keskin, E., Top, N., Doğaç, E., Baysal, Ö., & Emiroğlu, Ö. (2015). The role of environmental factors and genetic diversity on colonization success of a non-native fish, Lepomis gibbosus from western part of Turkey. Biochemical Systematics and Ecology, 58, 195-203.

Bandelt, H. J., Forster, P., & Röhl, A. (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16, 37-48.

Bauer, O. N. (1987). Key to the freshwater fish parasites of the USSR. Leningrad: Nauka.

Belkhir, K., Borsa, P., Chikhi, L., Raufaste, N., Bonhomme, F. (1996-2004). GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. In: Laboratoire Génome, Populations, Interactions, CNRS UMR;. p. 5000.

Benejam, L., Alcaraz, C., Sasal, P., Simon-Levert, G., & García-Berthou, E. (2009). Life history and parasites of the invasive mosquitofish (Gambusia holbrooki) along a latitudinal gradient. Biological Invasions, 11, 2265-2277.

Beverley-Burton, M. (1984). Monogenea and Turbellaria. In L. Margolis & Z. Kabata (Eds.), Guide to the parasites of fishes of Canada. Part I (pp. 5-209). Ottawa: Fisheries and Oceans, Scientific Information and Publication Branch.

Boettger, C. R. (1934). Der nordamerikanische Flusskrebs Cambarus affinis Say in Deutschland. Sitzungsberichte Gesellschaft Naturforschender Freunde zuBerlin, 4-7, 149-157.

Bush, A. O., Lafferty, K. D., Lotz, J. M., & Shostak, A. W. (1997). Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology, 83, 575-583.

Chalkowski, K., Lepczyk, C. A., & Zohdy, S. (2018). Parasite ecology of invasive species: Conceptual framework and new hypotheses. Trends in Parasitology, 34(8), 655-663.

Colautti, R. I., Ricciardi, A., Grigorovich, I. A., & MacIsaac, H. J. (2004). Is invasion success explained by the enemy release hypothesis? Ecology Letters, 7, 721-733.

Copp, G. H., & Fox, M. G. (2007). Growth and life history traits of introduced pumpkinseed (Lepomis gibbosus) in Europe, and the relevance to its potential invasiveness. In F. Gherardi (Ed.), Biological invaders in inland waters: Profiles, distribution, and threats. Invading nature - springer series In invasion ecology (Vol. 2). Dordrecht: Springer.

Copp, G. H., Wesley, K. J., & Vilizzi, L. (2005). Pathways of ornamental and aquarium fish introductions into urban ponds of Epping Forest (London, England): The human vector. Journal of Applied Ichthyology, 21(4), 263-274.

Cucherousset, J., Copp, G. H., Fox, M. G., Sterud, E., van Kleef, H. H., Verreycken, H., & Záhorská, E. (2009). Life-history traits and potential invasiveness of introduced pumpkinseed Lepomis gibbosus populations in northwestern Europe. Biological Invasions, 11, 2171-2180.

Dembski, S., Masson, G., Monnier, D., Wagner, P., & Pihan, J. C. (2006). Consequences of elevated temperatures on life-history traits of an introduced fish, pumpkinseed Lepomis gibbosus. Journal of Fish Biology, 69(2), 331-346.

Domagała, J., & Kondratowicz, A. (2006). Warunki środowiskowe wód kanałów zimnego i ciepłego Elektrowni" Dolna Odra" w drugiej połowie lat 90-tych. Rocznik Ochrona Środowiska, 8, 355-360 (in Polish).

Domagała, J., & Pilecka-Rapacz, M. (2007). Charakterystyka wód pochłodniczych Elektrowni Dolna Odra w latach 2004-2006. [The profile of post-cooling water from Dolna Odra power plant 2004-2006.]. Zeszyty Naukowe Wydziału Budownictwa i Inżynierii Środowiska; Politechnika Koszalińska, 23, 751-760 (in Polish).

Domagała, J., Czerniejewski, P., & Pilecka-Rapacz, M. (2017). Interpopulation variation in growth and life-history traits of the non-native juvenile pumpkinseed Lepomis gibbosus (L., 1758), in Cooling water of a Power Plant in the Lower Stretch of the Oder River, Poland. Rocznik Ochrona Środowiska, 19, 96-121.

Domagała, J., Migdalska, B., Łabęcka, A. M., & Pilecka-Rapacz, M. (2003). Anodonta woodiana (Lea, 1834) na Pomorzu Zachodnim. Acta Biologica-Uniwersytet Szczeciński, 10, 199-202.

Dunn, A. M. (2009). Parasites and biological invasions. Advances in Parasitology, 68, 161-184.

Earl, D. A., & von Holdt, B. M. (2012). Structure Harvester: A website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources, 4, 359-361.

Evanno, G., Regnaut, S., & Goudet, J. (2005). Detecting the number of clusters of individuals using the software structure: A simulation study. Molecular Ecology, 14, 2611-2620.

Faubet, P., Waples, R. S., & Gaggiotti, O. E. (2007). Evaluating the performance of a multilocus bayesian method for the estimation of migration rates. Molecular Ecology, 16, 1149-1166.

Foster, R., Peeler, E., Bojko, J., Clark, P. F., Morritt, D., Roy, H. E., … Bass, D. (2021). Pathogens co-transported with invasive non-native aquatic species: Implications for risk analysis and legislation. NeoBiota, 69, 79-102. https://doi.org/10.3897/neobiota.71358.

Fox, M. G., Vila-Gispert, A., & Copp, G. H. (2007). Life-history traits of introduced Iberian pumpkinseed Lepomis gibbosus relative to native populations. Can differences explain colonization success? Journal of Fish Biology, 71, 56-69.

Froese, R. & Pauly, D. (2021, November). FishBase. World Wide Web electronic publication. Retrieved from http://www.fishbase.org/.

Gendron, A. D., & Marcogliese, D. J. (2017). Enigmatic decline of a common fish parasite (Diplostomum spp.) in the St. Lawrence River: Evidence for a dilution effect induced by the invasive round goby. International Journal for Parasitology: Parasites & Wildlife, 6, 402-411.

Gendron, A. D., Marcogliese, D. J., & Thomas, M. (2012). Invasive species are less parasitized than native competitors, but for how long? The case of the round goby in the Great Lakes-St Lawrence Basin. Biological Invasions, 14, 367-384.

Georgiev, B., Biserkov, V., & Genov, T. (1986). In toto staining method for cestodes with iron acetocarmine. Helminthologia, 23, 279-281.

Georgieva, S., Soldánová, M., Pérez-del-Olmo, A., Dangel, D. R., Sitko, J., Sures, B., & Kostadinova, A. (2013). Molecular prospecting for European Diplostomum (Digenea: Diplostomidae) reveals cryptic diversity. International Journal for Parasitology, 43, 57-72.

Goswami, U., Molnár, K., Cech, G., Eiras, J. C., Bandyopadhyay, P. K., Ghosh, S., … Székely, C. (2021). Evidence of the American Myxobolus dechtiari was introduced along with its host in Europe: Molecular and histological data. International Journal for Parasitology: Parasites and Wildlife, 15, 51-57.

Gruszka, P. (1999). The river Odra estuary as a gateway for alien species immigration to the Baltic Sea basin. Acta Hydrochimica et Hydrobiologica, 27(5), 374-382.

Gusev, A. V., Dubinina, M. N., Raikova, E. V., Khotenkovskiy, I. A., Pugachev, O. N., & Ergens, R. (1985). Paraziticheskiye mnogokletochnye. Part 1. Monogenea. In O. N. Bauer (Ed.), Opredelitel parazitov presnovodnykh ryb fauny SSSR (pp. 1-425). Leningrad: Nauka.

Havlátová, L., Ondračková, M., & Přikrylová, I. (2015). Monogenean parasites of Lepomis gibbosus Linnaeus introduced into the river durance, France. Helminthologia, 52, 323-330.

Heese, T., & Przybyszewski, C. (1985). Pumpkinseed sunfsh, Lepomis gibbosus (L., 1758), (Pisces, Centrarchidae) in the lower Oder. Przegląd Zoologiczny, 29, 515-519 (In Polish).

Hockley, F. A., Williams, C. F., Reading, A. J., Taylor, N. G. H., & Cable, J. (2011). Parasite fauna of introduced pumpkinseed fish Lepomis gibbosus: First British record of Onchocleidus dispar (Monogenea). Diseases of Aquatic Organisms, 97, 65-73.

Hoffman, G. L. (1999). Parasites of North American freshwater fishes. Ithaca: Cornell University Press.

Hoffman, R. (1928). Sonnenfische (Eupomotis gibbosus L.) in den Nebenflussen der Oder (Vol. 25, p. 439). Braunschweig: Wochenschr. Aquar. Terrark.

Holčík, J. (1991). Fish introductions in Europe with particular reference to its central and eastern part. Canadian Journal of Fisheries and Aquatic Science, 48, 13-23.

Hothorn, T., Bretz, F., & Westfall, P. (2008). Simultaneous inference in general parametric models. Biometrical Journal, 50(3), 346-363.

Hubisz, M. J., Falush, D., Stephens, M., & Pritchard, J. K. (2009). Inferring weak population structure with the assistance of sample group information. Molecular Ecology Resources, 9, 1322-1332.

Jarvis, W. M., Nolan, C. B., & Robinson, B. W. (2021). Ecomorphological variation of pumpkinseed sunfish (Lepomis gibbosus) in exposed littoral shoreline habitat of a postglacial lake. Environmental Biology of Fishes, 104(2), 119-133.

Keane, R. M., & Crowley, M. J. (2002). Exotic plant invasions and the enemy release hypothesis. Trends in Ecology and Evolution, 17, 164-170.

Konečný, A., Popa, O. P., Bartáková, V., Douda, K., Bryja, J., Smith, C., … Reichard, M. (2018). Modelling the invasion history of Sinanodonta woodiana in Europe: Tracking the routes of a sedentary aquatic invader with mobile parasitic larvae. Evolutionary Applications, 11(10), 1975-1989.

Kopelman, N. M., Mayzel, J., Jakobsson, M., Rosenberg, N. A., & Mayrose, I. (2015). Clumpak: Aprogram for identifying clustering modes and packaging population structure inferences across K. Molecular Ecology Resources, 15, 1179-1191.

Kubiak, J., & Machula, S. (2013). Water thermal regimes in selected antropogenic resorvoirs in Western Pomerania. Oceanological and Hydrobiological Studies, 42(2), 155-163.

Kvach, Y., Jurajda, P., Bryjová, A., Trichkova, T., Ribeiro, F., Přikrylová, I., & Ondračková, M. (2017). European distribution for metacercariae of the north American digenean Posthodiplostomum cf. minimum centrarchi (Strigeiformes: Diplostomidae). Parasitology International, 66, 635-642.

Kvach, Y., Ondračková, M., Janáč, M., & Jurajda, P. (2016a). Methodological issues affecting the study of fish parasites. I. Duration of live fish storage prior to dissection. Diseases of Aquatic Organisms, 119(2), 107-115.

Kvach, Y., Ondračková, M., Janáč, M., & Jurajda, P. (2016b). Methodological issues affecting the study of fish parasites. II. Sampling method affects ectoparasite studies. Diseases of Aquatic Organisms, 121, 59-66.

Kvach, Y., Ondračková, M., Janáč, M., Krasnovyd, V., Seifertová, M., & Jurajda, P. (2019). Parasites of round goby, Neogobius melanostomus, currently invading the Elbe River. Oceanological and Hydrobiological Studies, 48, 56-65.

Kvach, Y., Ondračková, M., Kutsokon, Y., & Dzyziuk, N. (2018). New record of monogenean parasites on non-indigenous fishes in the Ukrainian Danube delta. BioInvasions Records, 7, 65-72.

Kvach, Y., Seifertová, M., Carassou, L., & Ondračková, M. (2020). First record of the American cestode Proteocephalus ambloplitis (Leidy, 1887) (Proteocephalidae) in Europe. Journal of Helminthology, 94, e144.

Kvach, Y., Tkachenko, M. Y., Bartáková, V., Zięba, G., & Ondračková, M. (2021). The role of the non-indigenous pumpkinseed Lepomis gibbosus (Actinopterygii: Centrarchidae) in the life cycle of Bothriocephalus claviceps (Cestoda: Bothriocephalidae) in Europe. Parasitology Research, 120, 3163-3171.

Lafferty, K. D., & Kuris, A. M. (1999). How environmental stress affects the impacts of Para-sites. Limnology and Oceanography, 44, 925-931.

Lewis, W. M., & Nickum, J. (1964). The effect of Posthodiplostomum minimum upon the body weight of the bluegill. The Progressive Fish-Culturist, 26, 121-123.

Lusk, S., Lusková, V., & Hanel, L. (2011). Black list of the alien invasive species in The Czech Republic. In S. Lusk & V. Lusková (Eds.), Biodiversity of fishes of The Czech Republic (VIII) (pp. 79-97). Brno: IVB ASCR (In Czech).

MacLeod, C. J., Paterson, A. M., Tompkins, D. M., & Runcán, R. P. (2010). Parasite lost - Do invaders miss the boat or drown on arrival? Ecology Letters, 13, 516-527.

Malmberg, G. (1970). The excretory systems and the marginal hooks as a basis for systematics of Gyrodactylus (Trematoda, Monogenea). Arkiv für Zoologie, 23, 1-235.

Maneepitaksanti, W., & Nagasawa, K. (2012). Actinocleidus fergusoni (Monogenea: Ancyrocephalidae), a gill parasite of bluegill (Lepomis macrochirus), new to Japan. Biogeography, 14, 93-97.

Maneepitaksanti, W., & Nagasawa, K. (2013). First record of Onchocleidus dispar (Monogenea: Ancyrocephalidae), a gill parasite of bluegill (Lepomis macrochirus), from Japan. Biogeography, 15, 67-71.

Marcogliese, D. J. (2004). Parasites: Small players with crucial roles in the ecological theatre. EcoHealth, 1, 151-164.

Masson, G., Vanacker, M., Fox, M., & Beisel, J. (2015). Impact of the cestode Triaenophorus nodulosus on the exotic Lepomis gibbosus and the autochthonous Perca fluviatilis. Parasitology, 142, 745-755.

Mazzone, A., Caffara, M., Gustinelli, A., Agnetti, F., Sgariglia, E., Lo Vaglio, G., … Fioravanti, M. L. (2019). Morphological and molecular characterization of larval and adult stages of Eustrongylides excisus (Nematoda: Dioctophymatoidea) with histophathological observations. Journal of Parasitology, 105, 882-889.

Meirmans, P. G. (2014). Nonconvergence in bayesian estimation of migration rates. Molecular Ecology Resources, 14, 726-733. https://doi.org/10.1111/1755-0998.12216.

Mierzejewska, K., Kvach, Y., Stańczak, K., Grabowska, J., Woźniak, M., Dziekońska-Rynko, J., & Ovcharenko, M. (2014). Parasites of non-native gobies in the Włocławek reservoir on the lower Vistula River, first comprehensive study in Poland. Knowledge and Management of Aquatic Ecosystems, 414, 1.

Mierzejewska, K., Kvach, Y., Woźniak, M., Kosowska, A., & Dziekońska-Rynko, J. (2012). Parasites of an Asian fish, the Chinese sleeper Perccottus glenii, in the Włocławek reservoir on the lower Vistula River, Poland: in search of the key species in the host expansion process. Comparative Parasitology, 79, 23-29.

Moravec, F. (2001). Checklist of metazoan parasites of fishes of The Czech Republic and the Slovak Republic (1873-2000). Praha: Akademia.

Moravec, F. (2013). Parasitic nematodes of freshwater fishes of Europe (2nd ed.). Prague: Academia.

Muroga, K., Yoshimatsu, T., & Kasahara, S. (1980). Urocleidus ferox (Monogenea: Dactylogyridae) from bluegill sunfish in Japan. Bulletin of the Japanese Society of Scientific Fisheries, 46, 27-30.

Neophitou, C., & Giapis, A. J. (1994). A study of the biology of pumpkinseed (Lepomis gibbosus (L.)) in Lake Kerkini (Greece). Journal of Applied Ichthyology, 10, 123-133.

Niewiadomska, K. (1986). Verification of the life cycles of Diplostomum spathaceum (Rudolphi, 1819) and D. pseudospathaceum Niewiadomska, 1984 (Trematoda, Diplostomidae). Systematic Parasitology, 8, 23-31.

Niewiadomska, K. (2003). Pasożyty ryb Polski (klucze do oznaczenia), Przywry - Digenea. Warszawa: Polskie Towarzystwo Parazytologiczne.

Nędzarek, A., Tórz, A., Kubiak, J., & Machula, S. (2011). Habitat conditions for the ichthyofauna in anthropogenic Szmaragdowe Lake (Szczecin, Poland) in 2009-2010. In Fish Management in a Variable Water Environment (pp. 193-200). Olsztyn: Agencja Wydawnicza "Agri" SC.

Ojaveer, H., Turovski, A., & Nõomaa, K. (2020). Parasite infection of the non-indigenous round goby (Neogobius melanostomus) in the Baltic Sea. Aquatic Invasions, 15, 160-176.

Ondračková, M., Bartáková, V., Kvach, Y., Bryjová, A., Trichkova, T., Ribeiro, F., … Jurajda, P. (2021). Parasite infection reflects host genetic diversity in the pumpkinseeds non-native range. Hydrobiologia, 848, 2169-2187.

Ondračková, M., Dávidová, M., Přikrylová, I., & Pečínková, M. (2011). Monogenean parasites of introduced pumpkinseed Lepomis gibbosus (Centrarchidae) in the Danube River basin. Journal of Helminthology, 85, 435-441.

Ondračková, M., Fojtů, J., Seifertová, M., Kvach, Y., & Jurajda, P. (2019). Non-native parasitic copepod Neoergasilus japonicus (Harada, 1930) utilizes non-native fish host Lepomis gibbosus (L.) in the floodplain of the river Dyje (Danube basin). Parasitology Research, 118, 57-62.

Ondračková, M., Kvach, Y., Martens, A., & Jurajda, P. (2019). Limited parasite acquisition by non-native Lepomis gibbosus (Antinopterygii: Centrarchidae) in two ponds at the upper Rhine in Germany. Journal of Helminthology, 93, 453-460.

Ondračková, M., Valová, Z., Hudcová, I., Michálková, V., Šimková, A., Borcherding, J., & Jurajda, P. (2015). Temporal effects on host-parasite associations in four naturalized goby species living in sympatry. Hydrobiologia, 746, 233-243.

Ondračková, M., Všetičková, L., Adámek, Z., Kopeček, L., & Jurajda, P. (2019). Ecological plasticity of tubenose goby, a small invader in south Moravian waters. Hydrobiologia, 829, 217-235.

Ondračková, M., Šimková, A., Civáňová, K., Vyskočilová, M., & Jurajda, P. (2012). Parasite diversity and microsatellite variability in native and introduced populations of four Neogobius species (Gobiidae). Parasitology, 139, 1493-1505.

Panov, V. E., Alexandrov, B., Arbačiauskas, K., Binimelis, R., Copp, G. H., Grabowski, M., … Son, M. O. (2009). Assessing the risks of aquatic species invasions via European inland waterways: From concepts to environmental indicators. Integrated Environmental Assessment and Management, 5, 110-126.

Pappenheim, P. (1927). Eupomotis gibbosus (L.) in der Oder bei Crossen. SB. Schriften der Berlinischen Gesellschaft Naturforschender Freunde, 1926, 62-63.

Peakall, R., & Smouse, P. E. (2006). GenAlEx 6: Genetic analysis in excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288-295.

Peakall, R., & Smouse, P. E. (2012). GenAlEx 6.5: Genetic analysis in excel. Population genetic software for teaching and research - An update. Bioinformatics, 28, 2537-2539.

Piasecki, W., & Falandysz, M. (1994). Preliminary survey on parasite fauna of pumpkinseed sunfish. Acta Ichthyologica et Piscatoria, 24, 87-100.

Piechocki, A., & Potocki, M. (1976). Nowe stanowiska Physa acuta Drap., Gyraulus acronicus (Fe.) (Gastropoda, Basommatophora) w Parku Ludowym w Łodzi. Acta Universitatis Lodziensis, 9, 49-55.

Pilecka-Rapacz, M., & Sobecka, E. (2008). Parasitic nematodes of pumpkinseed sunfish (Lepomis gibbosus L., 1758) from warm-water canal of a power plant in Szczecin, Poland. Wiadomości Parazytologiczne, 54, 213-216.

Porębski, J., & Małkiewicz, W. (1995). Lepomis gibbosus L. w wodach Zalewu Szczecińskiego. Komunikaty Rybackie, 1, 24.

Poulin, R., & Mouillot, D. (2003). Host introductions and the geography of parasite taxonomic diversity. Journal of Biogeography, 30, 837-845.

Poulin, R. (2017). Invasion ecology meets parasitology: Advances and challenge. International Journal for Parasitology: Parasites and Wildlife, 6(3), 361-363.

Power, A. G., & Mitchell, C. E. (2004). Pathogen spillover in disease epidemics. American Naturalist, 164, S79-S89.

Poznańska-Kakareko, M., Wiśniewski, K., Szarmach, D., Witkowska, A., Kakareko, T., & Kobak, J. (2021). Importance of substratum quality for potential competitive niche overlap between native and invasive unionid mussels in Europe. Science of the Total Environment, 799, 149345.

R Core Team. (2021). R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing Retrieved from: https://www.R-project.org/.

Rambaut, A., & Drummond, A. (2004). Trace v1.6. Available: http://beast.bio.ed.ac.uk/tracer.

Rannala, B. (2007). BayesAss 3.0 User's Manual (update to 28 Sept 2015). University of California Davis.

Raymond, M., & Rousset, F. (1995). GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism. Journal of Heredity, 86, 248-249.

Roberts, L. S., & Janovy, J. (2000). Gerald D. Schmidt and Larry S. Roberts' Foundations of Parasitology, 61 h Ed. Boston: McGraw-Hill International Editions.

Rousset, F. (2008). Genepop'007: A complete re-implementation of the Genepop software for windows and Linux. Molecular Ecoloy Resources, 8, 103-106.

Rubtsova, N. Y. (2015). First record of Onchocleidus dispar, an alien monogenean from introduced pumpkinseed fish Lepomis gibbosus (Pisces, Centrarchidae) in Ukraine. Scientia Parasitologica, 16, 83-88.

Schreck, C. B., & Moyle, P. B. (1990). Methods for fish biology. Bethesda, MD: American Fisheries Society.

Slynko, E. E., Novitsky, R. A., Bangs, M. R., Douglas, M. R., Douglas, M. E., Khrystenko, D. S., … Slynko, Y. V. (2014). Phylogeography and phenotypic diversity of the pumpkinseed sunfish Lepomis gibbosus (Linnaeus, 1758) of the Northern Black Sea coast. Russian Journal of Genetics, 50, 1285-1293.

Sterud, E., & Jørgensen, A. (2006). Pumpkinseed Lepomis gibbosus (Linnaeus, 1758) (Centrarchidae) and associated parasites introduced to Norway. Aquatic Invasions, 1, 278-280.

Stoyanov, B., Georgieva, S., Pankov, P., Kudlai, O., Kostadinova, A., & Georgiev, B. B. (2017). Morphology and molecules reveal the alien Posthodiplostomum centrarchi Hoffman, 1958 as the third species of Posthodiplostomum Dubois, 1936 (Digenea: Diplostomidae) in Europe. Systematic Parasitology, 94, 1-20.

Šumer, S., Kováč, V., Povž, M., & Slatner, M. (2005). External morphology of a Slovenian population of pumpkinseed Lepomis gibbosus (L.) from a habitat with extreme thermal conditions. Journal of Applied Ichthyology, 21, 306-311.

Telfer, S., & Bown, K. (2012). The effects of invasion on parasite dynamics and communities. Functional Ecology, 26, 1288-1299.

Tomeček, J., Kováč, V., & Katina, S. (2007). The biological flexibility of the pumpkinseed: A successful colonizer throughout Europe. In Biological Invaders in Inland Waters: Profiles, Distribution, and Threats (pp. 307-336). Dordrecht: Springer.

Torchin, M. E., Lafferty, K. D., Dobson, A. P., McKenzie, V. J., & Kuris, A. M. (2003). Introduced species and their missing parasites. Nature, 421, 628-629.

Truter, M., Přikrylová, I., Weyl, O. L. F., & Smit, N. J. (2017). Co-introduction of ancyrocephalid monogeneans on their invasive host, the largemouth bass, Micropterus salmoides (Lacepede, 1802) in South Africa. International Journal for Parasitology: Parasites and Wildlife, 6, 420-429.

van Kleef, H. H., & Jongejans, E. (2014). Identifying drivers of pumpkinseed invasiveness using population models. Aquatic Invasions, 9(3), 315-326.

Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R., & Hebert, P. D. (2005). DNA barcoding Australia's fish species. Philosophical transactions of the Royal Society of London Series B, Biological sciences, 360, 1847-1857.

White, M. (2019). White grub in bluegill in the Sangamon River: Impact of sewage effluent and flow regime on infection parameters. (Master Thesis, Eastern Illinois University); 4433.

Wilson, G., & Rannala, B. (2003). Bayesian inference of recent migration rates using multilocus genotypes. Genetics, 163, 1177-1191.

Witkowski, A. (1989). Introdukowane ryby w polskich wodach i ich wpływ na środowisko. Przeglad Zoologiczny, 33, 583-598.

Yavno, S., Gobin, J., Wilson, C. C., Vila-Gispert, A., Copp, G. H., & Fox, M. (2020). New and olds world phylogeography of pumkinseed Lepomis gibbosus (Linnaeus, 1758): The north American origin of introduced populations in Europe. Hydrobiologia, 847, 345-364.

Zięba, G., Dukowska, M., Przybylski, M., Fox, M. G., & Smith, C. (2018). Parental care compromises feeding in the pumpkinseed (Lepomis gibbosus). Science of the Nature, 105, 26. https://doi.org/10.1007/s00114-018-1554-0.

Zięba, G., Leśniak, M., Marszał, L., Janic, B., & Błońska, D. (2016). Bass słoneczny (Lepomis gibbosus) w dorzeczu dolnej Odry, występowanie i regulacja liczebności populacji. [Pumpkinseed (Lepomis gibbosus) in the lower Oder River catchement, distribution and regulation of population size.]. Roczniki Naukowe Polskiego Związku Wędkarskiego, 29, 89-103 (In Polish).

Zięba, G., Vilizzi, L., & Copp, G. H. (2020). How likely is Lepomis gibbosus to become invasive in Poland under conditions of climate warming? Acta Ichthyologica et Piscatoria, 50, 37-51.

Comparative Analysis of Parasite Load on Recently Established Invasive Pumpkinseed Lepomis gibbosus (Actinopterygii: Centrarchidae) in Europe