Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
36653086
DOI
10.1016/bs.mcb.2022.07.015
PII: S0091-679X(22)00106-6
Knihovny.cz E-resources
- Keywords
- B3Z, Confocal microscopy, Conjugation assay, Flow cytometry, Immunological synapse, MutuDC,
- MeSH
- Lymphocyte Activation MeSH
- Antigen-Presenting Cells * MeSH
- Immunological Synapses physiology MeSH
- Actin Cytoskeleton MeSH
- Receptors, Antigen, T-Cell MeSH
- T-Lymphocytes * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Receptors, Antigen, T-Cell MeSH
Interaction between an antigen-presenting cell and a T cell, and their subsequent conjugation are a prerequisite for the formation of the immunological synapse and productive, antigen-dependent activation of T cells. This initial interaction is accompanied by recognition of the presented antigen by the T cell receptor, and by changes in the morphology of the interacting cells and in actin cytoskeleton structure in the site of interaction. The experimental protocol below describes a simple assay for quantitative assessment of antigen-presenting cells-T cell conjugation using confocal microscopy or flow cytometry.
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