Osteoarthritisis a highly prevalent musculoskeletal disorder characterized by degradation of cartilage and synovial fluid (SF). Platelet derivatives as platelet-rich plasma (PRP) and platelet lysate have great potential in the treatment of osteoarthritis because they contain biologically active substances including growth factors (GFs). Rapid release of GFs and their short biological half-life are factors that can limit the therapeutic impact of PRP therapy. Herein, the first work that describes hydrogels based on polyaldehyde derivative of hyaluronic acid (HA-OX) as carriers of platelet derivatives for in situ applications is presented, which can be a possible solution to the problem. HA-OX hydrogels containing 50% (w/w) of PRP or platelet lysate can be injected using a syringe due to low viscosity(<10 Pa s) and injection force (<20 N), and reach elastic modulus up to 2000 Pa. Insulin-like GF-1 and Platelet-derived GF-AB release from HA-OX hydrogels (mesh size 297-406 nm) by Fickian and non-Fickian diffusion respectively. The released PRP GFs maintain their ability to induce cell proliferation (87%-92%). Based on the obtained results, the unique concept of a new material that can restore viscoelastic properties of SF and at the same time gradually deliver GFs from platelet derivatives is designed.

Biocev 1st Faculty of Medicine Charles University Průmyslová 595 Vestec 25250 Czech Republic

Contipro a s Dolní Dobrouč 401 Dolní Dobrouč 56102 Czech Republic

Department of Pediatrics and Inherited Metabolic Disorders 1st Faculty of Medicine Charles University and General University Hospital Prague Ke Karlovu 455 Prague 120 00 Czech Republic

Zobrazit více v PubMed

M. F. Barbe, J. Driban, A. E. Barr, S. N. Popoff, F. F. Safadi, in Bone Pathology, (Ed: J. S. Khurana), Humana Press, Totowa, NJ 2009, p. 51.

GBD_2019_Diseases_and_Injuries_Collaborators, Lancet 2020, 396, 1204.

K. Pavelka, Čes. Revmatol. 2012, 20, 138.

H. Long, Q. Liu, H. Yin, K. Wang, N. Diao, Y. Zhang, J. Lin, A. Guo, Arthritis Rheumatol. 2022, 74, 1172.

I. Haq, E. Murphy, J. Dacre, Postgrad. Med. J. 2003, 79, 377.

E. Balazs, Knee 1974, 63.

E. A. Balazs, D. Watson, I. F. Duff, S. Roseman, Arthritis Rheum. 1967, 10, 357.

M. Yoshida, S. Sai, K. Marumo, T. Tanaka, N. Itano, K. Kimata, K. Fujii, Arthritis Res. Ther. 2004, 6, R514.

D. Hamerman, H. Schuster, J. Clin. Invest. 1958, 37, 57.

B. Decker, W. F. McGuckin, B. F. McKenzie, C. H. Slocumb, Clin. Chem. 1959, 5, 465.

P. Mathieu, T. Conrozier, E. Vignon, Y. Rozand, M. Rinaudo, Clin. Orthop. Relat. Res. 2009, 467, 3002.

H. Yamada, M. Morita, O. Henmi, S. Miyauchi, Y. Yoshida, T. Kikuchi, N. Terada, O. Washimi, Y. Washimi, T. Seki, Arch. Orthop. Trauma Surg. 2000, 120, 521.

D. Mazzucco, R. Scott, M. Spector, Biomaterials 2004, 25, 4433.

T. J. Brown, U. B. Laurent, J. R. Fraser, Exp. Physiol. 1991, 76, 125.

J. R. Fraser, T. C. Laurent, U. B. Laurent, J. Intern. Med. 1997, 242, 27.

K. N. Antonas, J. R. Fraser, K. D. Muirden, Ann. Rheum. Dis. 1973, 32, 103.

K. S. Girish, K. Kemparaju, Life Sci. 2007, 80, 1921.

N. E. Larsen, H. D. Dursema, C. T. Pollak, E. M. Skrabut, J. Biomed. Mater. Res., Part B 2012, 100, 457.

K. Edsman, R. Hjelm, H. Lärkner, L. I. Nord, A. Karlsson, Å. Wiebensjö, A. U. Höglund, A. H. Kenne, J. Näsström, Cartilage 2011, 2, 384.

L. W. Moreland, Arthritis Res. Ther. 2003, 5, 54.

S. A. Raeissadat, S. M. Rayegani, H. Hassanabadi, M. Fathi, E. Ghorbani, M. Babaee, K. Azma, Clin. Med. Insights: Arthritis Musculoskeletal Disord. 2015, 8, 1.

G. Filardo, E. Kon, A. Di Martino, B. Di Matteo, M. L. Merli, A. Cenacchi, P. M. Fornasari, M. Marcacci, BMC Musculoskeletal Disord. 2012, 13, 229.

R. Civinini, L. Nistri, C. Martini, B. Redl, G. Ristori, M. Innocenti, Clin. Cases Miner. Bone Metab. 2013, 10, 26.

L. A. Fortier, J. U. Barker, E. J. Strauss, T. M. McCarrel, B. J. Cole, Clin. Orthop. Relat. Res. 2011, 469, 2706.

E. N. Blaney Davidson, E. L. Vitters, P. L. van Lent, F. A. van de Loo, W. B. van den Berg, P. M. van der Kraan, Arthritis Res. Ther. 2007, 9, R102.

R. F. Loeser, G. Shanker, C. S. Carlson, J. F. Gardin, B. J. Shelton, W. E. Sonntag, Arthritis Rheum. 2000, 43, 2110.

J. L. Ellsworth, J. Berry, T. Bukowski, J. Claus, A. Feldhaus, S. Holderman, M. S. Holdren, K. D. Lum, E. E. Moore, F. Raymond, H. Ren, P. Shea, C. Sprecher, H. Storey, D. L. Thompson, K. Waggie, L. Yao, R. J. Fernandes, D. R. Eyre, S. D. Hughes, Osteoarthritis Cartilage 2002, 10, 308.

T. Deng, S. Huang, S. Zhou, L. He, Y. Jin, J. Microencapsulation 2007, 24, 163.

G. Z. Zhao, L. Q. Zhang, Y. Liu, J. Fang, H. Z. Li, K. H. Gao, Y. Z. Chen, Mol. Med. Rep. 2016, 14, 897.

K. Lee, E. A. Silva, D. J. Mooney, J. R. Soc., Interface 2011, 8, 153.

C. Durante, F. Agostini, L. Abbruzzese, R. T. Toffola, S. Zanolin, C. Suine, M. Mazzucato, Vox Sang. 2013, 105, 129.

G. Huang, H. Huang, J. Controlled Release 2018, 278, 122.

E. A. Balazs, in Chemistry and Biology of Hyaluronan, Elsevier Science Ltd, Oxford 2004, p. 415.

J. F. S. D. Lana, A. Weglein, S. E. Sampson, E. F. Vicente, S. C. Huber, C. V. Souza, M. A. Ambach, H. Vincent, A. Urban-Paffaro, C. M. K. Onodera, J. M. Annichino-Bizzacchi, M. H. A. Santana, W. D. Belangero, J. Stem Cells Regen. Med. 2016, 12, 69.

W. Yu, P. Xu, G. Huang, L. Liu, Exp. Ther. Med. 2018, 16, 2119.

F. Lee, K. H. Bae, M. Kurisawa, Biomed. Mater. 2015, 11, 014101.

S. Trombino, C. Servidio, F. Curcio, R. Cassano, Pharmaceutics 2019, 11, 407.

N. K. Devaraj, ACS Cent. Sci. 2018, 4, 952.

J. Xu, Y. Liu, S.-H. Hsu, Molecules 2019, 24, 3005.

P. Šedová, R. Buffa, S. Kettou, G. Huerta-Angeles, M. Hermannová, V. Leierová, D. Šmejkalová, M. Moravcová, V. Velebný, Carbohydr. Res. 2013, 371, 8.

G. N. Grover, R. L. Braden, K. L. Christman, Adv. Mater. 2013, 25, 2937.

R. Buffa, L. Odstrčilová, P. Šedová, I. Basarabová, J. Novotný, V. Velebný, Carbohydr. Polym. 2018, 189, 273.

invs., R. Buffa, S. Kettou, L. Pospíšilová, M. Němcová, V. Velebný (Contipro a.s.), EP 2510017B1 2015.

invs., R. Buffa, S. Kettou, L. Simunkova, G. Huerta-Angeles, D. Chládková, V. Velebný (Contipro a.s.), EP 2510016B1 2012.

K. Yasuda, R. C. Armstrong, R. E. Cohen, Rheol. Acta 1981, 20, 163.

M. N. Collins, C. Birkinshaw, J. Appl. Polym. Sci. 2008, 109, 923.

R. W. Korsmeyer, R. Gurny, E. Doelker, P. Buri, N. A. Peppas, Int. J. Pharm. 1983, 15, 25.

N. Bellamy, J. Campbell, V. Robinson, T. Gee, R. Bourne, G. Wells, Cochrane Database Syst. Rev. 2006, 2006, Cd005321.

M. S. Dhillon, S. Patel, T. Bansal, J. Clin. Orthop. Trauma 2019, 10, 32.

F. Cilurzo, F. Selmin, P. Minghetti, M. Adami, E. Bertoni, S. Lauria, L. Montanari, AAPS PharmSciTech 2011, 12, 604.

R. Jin, C. Hiemstra, Z. Zhong, J. Feijen, Biomaterials 2007, 28, 2791.

B. M. Manzini, L. M. R. Machado, P. Y. Noritomi, J. V. L. da Silva, J. Biosci. 2021, 46, 17.

N. Kashyap, N. Kumar, M. N. Kumar, Crit. Rev. Ther. Drug Carrier Syst. 2005, 22, 107.

J. Li, D. J. Mooney, Nat. Rev. Mater. 2016, 1, 16071.

M. Qiu, D. Chen, C. Shen, J. Shen, H. Zhao, Y. He, Int. J. Mol. Sci. 2016, 17, 1001.

G. Rees, J. McCutcheon, “Clinical Procedures for Safer Patient Care”, BCcampus, Canada 2016.

L. Dougherty, S. Lister, The Royal Marsden Manual of Clinical Nursing Procedures, 8th ed., John Wiley & Sons Ltd, UK 2011.

A. Rył, P. Owczarz, Polymers 2020, 12, 2260.

R. P. Watt, H. Khatri, A. R. G. Dibble, Int. J. Pharm. 2019, 554, 376.

M. H. Chen, L. L. Wang, J. J. Chung, Y. H. Kim, P. Atluri, J. A. Burdick, ACS Biomater. Sci. Eng. 2017, 3, 3146.

A. Vo, M. Doumit, G. Rockwell, J. Med. Eng. 2016, 2016, 5162394.

K. J. Wågø, T. I. Skarsvåg, J. S. Lundbom, L. F. Tangen, S. Ballo, T. Hjelseng, V. Finsen, Scand. J. Plast. Reconstr. Surg. 2016, 50, 115.

K. Okamoto, N. Ami, H. Oshima, Pain Res. 2012, 27, 215.

D. Rebenda, M. Vrbka, P. Čípek, E. Toropitsyn, D. Nečas, M. Pravda, M. Hartl, Materials 2020, 13, 2659.

D. Rebenda, M. Vrbka, D. Nečas, E. Toropitsyn, S. Yarimitsu, P. Čípek, M. Pravda, M. Hartl, Tribol. Int. 2021, 160, 107030.

H. Sundaram, B. Voigts, K. Beer, M. Meland, Dermatol. Surg. 2010, 36, 1859.

M. Muhammad, C. Willems, J. Rodríguez-Fernández, Biomolecules 2020, 10.

M. Weis, J. Shan, M. Kuhlmann, T. Jungst, J. Tessmar, J. Groll, Gels 2018, 4.

G. Singh, A. Chanda, Biomed. Mater. 2021, 16, 062004.

J. L. Vanderhooft, M. Alcoutlabi, J. J. Magda, G. D. Prestwich, Macromol. Biosci. 2009, 9, 20.

X. Liu, Y. Yang, X. Niu, Q. Lin, B. Zhao, Y. Wang, L. Zhu, Acta Biomater. 2017, 62, 179.

S. Wei, P. Xu, Z. Yao, X. Cui, X. Lei, L. Li, Y. Dong, W. Zhu, R. Guo, B. Cheng, Acta Biomater. 2021, 124, 205.

B. Qian, Q. Yang, M. Wang, S. Huang, C. Jiang, H. Shi, Q. Long, M. Zhou, Q. Zhao, X. Ye, Bioact. Mater. 2021, 7, 401.

E. Jooybar, M. J. Abdekhodaie, M. Alvi, A. Mousavi, M. Karperien, P. J. Dijkstra, Acta Biomater. 2019, 83, 233.

Y. Zhan, W. Fu, Y. Xing, X. Ma, C. Chen, Mater. Sci. Eng., C 2021, 127, 112208.

R. Censi, C. Casadidio, S. Deng, M. R. Gigliobianco, M. G. Sabbieti, D. Agas, F. Laus, P. Di Martino, Int. J. Mol. Sci. 2020, 21, 1399.

F. Zamboni, E. Ryan, M. Culebras, M. N. Collins, Carbohydr. Polym. 2020, 245, 116501.

J. B. Leach, K. A. Bivens, C. W. P. Jr, C. E. Schmidt, Biotechnol. Bioeng. 2003, 82, 578.

E. Tsanaktsidou, O. Kammona, C. Kiparissides, Eur. Polym. J. 2019, 114, 47.

F. Lee, J. E. Chung, M. Kurisawa, J. Controlled Release 2009, 134, 186.

M. Martini, P. S. Hegger, N. Schädel, B. B. Minsky, M. Kirchhof, S. Scholl, A. Southan, G. E. M. Tovar, H. Boehm, S. Laschat, Materials 2016, 9, 810.

D. Burnett, S. M. Wood, A. R. Bradwell, Bioch. Biophys. Acta 1976, 427, 231.

S. Handtke, T. Thiele, J. Thromb. Haemostasis 2020, 18, 1256.

V. Pavlovic, M. Ciric, V. Jovanovic, P. Stojanovic, Open Med. 2016, 11, 242.

E. Anitua, M. Sánchez, R. Prado, G. Orive, Platelets 2011, 22, 473.

Y. Taniguchi, T. Yoshioka, J. Exp. Ortop. 2019, 6, 4.

H. S. Cho, I. H. Song, S. Y. Park, M. C. Sung, M. W. Ahn, K. E. Song, Korean J. Lab. Med. 2011, 31, 212.

A. K. McClain, T. M. McCarrel, BMC Vet. Res. 2019, 15, 292.

W. Yin, X. Qi, Y. Zhang, J. Sheng, Z. Xu, S. Tao, X. Xie, X. Li, C. Zhang, J. Transl. Med. 2016, 14, 73.

J. W. Lee, O. H. Kwon, T. K. Kim, Y. K. Cho, K. Y. Choi, H. Y. Chung, B. C. Cho, J. D. Yang, J. H. Shin, Arch. Plast. Surg. 2013, 40, 530.

J. Siepmann, N. A. Peppas, Adv. Drug Delivery Rev. 2001, 48, 139.

J. V. Nauman, P. G. Campbell, F. Lanni, J. L. Anderson, Biophys. J. 2007, 92, 4444.

D. T. Graves, D. L. Cochran, Crit. Rev. Oral Biol. Med. 1990, 1, 17.

M. B. Schmidt, E. H. Chen, S. E. Lynch, Osteoarthritis Cartilage 2006, 14, 403.

D. B. Pike, S. Cai, K. R. Pomraning, M. A. Firpo, R. J. Fisher, X. Z. Shu, G. D. Prestwich, R. A. Peattie, Biomaterials 2006, 27, 5242.

E. Kobayashi, L. Flückiger, M. Fujioka-Kobayashi, K. Sawada, A. Sculean, B. Schaller, R. J. Miron, Clin. Oral Investig. 2016, 20, 2353.

X. Ren, M. Zhao, B. Lash, M. M. Martino, Z. Julier, Front. Bioeng. Biotechnol. 2020, 7, 469.

E. Toropitsyn, M. Pravda, D. Rebenda, I. Ščigalková, M. Vrbka, V. Velebný, J. Biomed. Mater. Res., Part B 2022, 110, 2595.

C. Messina, G. Guglielmi, D. Orlandi, A. Corazza, G. Mauri, L. M. Sconfienza, Curr. Radiol. Rep. 2017, 5, 1.

P. Geborek, T. Saxne, D. Heinegård, F. A. Wollheim, J. Rheumatol., Suppl. 1988, 15, 91.

M. Kong, K. Hashim, P. Lin, G. Coestesquis, A. Xu, F. Lebes, C. Ting, J. Biosci. Med. 2019, 07, 76.

V. B. Kraus, T. V. Stabler, S. Y. Kong, G. Varju, G. McDaniel, Osteoarthritis Cartilage 2007, 15, 1217.

J. R. Levick, Arthritis Rheum. 1981, 24, 1550.

M. M. Bradford, Anal. Biochem. 1976, 72, 248.

P. J. Flory, Principles of Polymer Chemistry, Cornell University Press, USA 1953.

N. A. Peppas, P. Bures, W. Leobandung, H. Ichikawa, Eur. J. Pharm. Biopharm. 2000, 50, 27.