Porcine oocytes matured in a chemically defined medium are transcriptionally active
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
37001226
DOI
10.1016/j.theriogenology.2023.03.019
PII: S0093-691X(23)00109-7
Knihovny.cz E-resources
- Keywords
- Chemically defined medium, Maturation in vitro, Porcine oocyte, Transcriptome,
- MeSH
- Cell Nucleus metabolism MeSH
- In Vitro Oocyte Maturation Techniques * veterinary methods MeSH
- RNA, Messenger genetics metabolism MeSH
- Oocytes * metabolism MeSH
- Swine MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- RNA, Messenger MeSH
The statement that fully-grown porcine oocytes (oocytes from follicles with diameter from 3 to 6 mm) are transcriptionally quiescent is not as strongly supported as it was before. Currently, we know that there is a difference between the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and MII oocytes matured in the chemically defined medium FLI. Oocytes were sequenced, and the results were subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). We detected multiple differentially transcribed mRNAs, of which many were upregulated. Among them we found mRNAs necessary for protein production, mitochondrial functions and cytoplasmic maturation. Collectively, these data support the hypothesis that transcription activity in fully-grown porcine oocytes is necessary for key processes during their successful maturation in vitro in a chemically defined maturation medium.
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