Activation of TLRs by Opportunistic Fungi in Lung Organoids
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Aspergillus fumigatus, Fungal infections, Fungi, Organoids, PGG, TLR, WGP, iPSC, β-glucan,
- MeSH
- Aspergillus fumigatus * MeSH
- buněčná diferenciace MeSH
- houby * MeSH
- interakce hostitele a patogenu MeSH
- lidé MeSH
- organoidy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Organoid cultures may express several types of pattern-recognition receptors and in particular toll-like receptors, representing an extremely efficient and innovative system to understand how pathogen-associated molecular patterns exposure may affect the immunity, the growth, or differentiation of complex tissues. Here, we describe how to generate lung organoids from human-induced pluripotent stem cells. Three-dimensional (3D) cultures are then stimulated with different toll-like receptor ligands derived from fungi or with Aspergillus fumigatus. RNA sequencing may be performed upon organoid cultures to understand host-pathogen innate immune interactions.
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
Department of Medicine and Surgery University of Perugia Perugia Italy
Institute of Haematology and Blood Transfusion Prague Czech Republic
International Clinical Research Centre St Anne's University Hospital Brno Brno Czech Republic
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