Analysis of glycosylated cardiolipins from thermophilic bacteria using GC-MS and LC-ESI-MS/MS methods
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
37871419
DOI
10.1016/j.jpba.2023.115800
PII: S0731-7085(23)00569-1
Knihovny.cz E-zdroje
- Klíčová slova
- Electrospray mass spectrometry, Glucosyl-cardiolipins, Hydrophilic interaction liquid chromatography, Partially methylated alditol acetates, Thermophilic bacteria,
- MeSH
- Bacteria MeSH
- chromatografie kapalinová metody MeSH
- glukosa MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- kardiolipiny * analýza MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- tandemová hmotnostní spektrometrie * metody MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glukosa MeSH
- kardiolipiny * MeSH
Unusual glucose-substituted cardiolipins (Glcx-CLs) in three genera of thermophilic bacteria, having more than one glycosidically linked glucose to the hydroxyl of the central glycerol of Glcx-CLs were identified for the first time in thermophilic bacteria of the genera Geobacillus, Meiothermus, and Thermus. The number of glucoses reached up to five units. The structure of glycosidically linked oligosaccharides was determined based on shotgun analysis MS (electrospray high-resolution tandem mass spectrometry), partially methylated alditol acetates were identified by GC-MS, both electron ionization (EI) and positive chemical ionization (PCI), hydrophilic interaction liquid chromatography (HILIC) separation and identification of CLs glycosides by high resolution MS-ESI, and digestion by specific glycosidases.
Institute of Microbiology Czech Academy of Sciences Vídeňská 1083 Prague 14200 Czech Republic
Research Institute of Brewing and Malting Lípová 511 Prague 12044 Czech Republic
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