The chemical profile and the levels of AVNs in oat varieties after germination have been examinated. In the present study, 12 distinct oat varieties were germinated for 0-192 h and a total of 28 AVNs and 3 AVN-hexosides were determined in these samples. Among them, three novel AVNs were synthesized (AVN 1a, AVN 2a, and AVN 2ad), characterized using NMR techniques (1D- and 2D-NMR), and assessed in real samples for the first time. The most abundant AVNs in the samples were AVN 2c, AVN 2p, AVN 2f, and their long-chained analogues AVN 2 cd, AVN 2pd, AVN 2fd, together representing 75-85 % of the total AVNs content. The highest total AVN level was observed on average after 48-72 h of germination time and it reached a value 1-1.2 mg/g. Out of 12 investigated oat varieties, CDC Boyer, Diadem, and Rozmar have proved to be the most suitable genotypes for germination.

Department of Chemistry Division of Organic Chemistry University of Natural Resources and Life Sciences Vienna Muthgasse 18 1190 Vienna Austria

Gene Bank Crop Research Institute Drnovská 507 73 161 06 Prague 6 Ruzyně Czech Republic

Institute for Animal Nutrition and Feed AGES Austrian Agency for Health and Food Safety GmbH Spargelfeldstr 192 1220 Vienna Austria

Institute of Food Technology BOKU University of Natural Resources and Life Sciences Vienna Muthgasse 18 1190 Vienna Austria

Institute of Physiology Czech Academic of Sciences Vídeňská 1084 142 00 Prague 4 Czech Republic

Institute of Plant Breeding BOKU University of Natural Resources and Life Sciences Vienna Konrad Lorenz Str 24 3430 Tulln an der Donau Austria

Quality of Plant Products Crop Research Institute Drnovská 507 73 161 06 Prague 6 Ruzyně Czech Republic

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