This study evaluated how exposure to the ubiquitous air pollution component, ultrafine particles (UFPs), alters the olfactory bulb (OB) transcriptome. The study utilised a whole-body inhalation chamber to simulate real-life conditions and focused on UFPs due to their high translocation and deposition ability in OBs as well as their prevalence in ambient air. Female C57BL/6J mice were exposed to clean air or to freshly generated combustion derived UFPs for two weeks, after which OBs were dissected and mRNA transcripts were investigated using RNA sequencing analysis. For the first time, transcriptomics was applied to determine changes in mRNA expression levels occurring after subacute exposure to UFPs in the OBs. We found forty-five newly described mRNAs to be involved in air pollution-induced responses, including genes involved in odorant binding, synaptic regulation, and myelination signalling pathway, providing new gene candidates for future research. This study provides new insights for the environmental science and neuroscience fields and nominates future research directions.

A 1 Virtanen Institute for Molecular Sciences University of Eastern Finland Kuopio Finland

Department of Computer Science Faculty of Electrical Engineering Czech Technical University Prague Jugoslávských partyzánů 1580 3 Prague 160 00 Czech Republic

Department of Environmental and Biological Sciences University of Eastern Finland Kuopio Finland

Department of Genetic Toxicology and Epigenetics Institute of Experimental Medicine CAS Vídeňská 1083 Prague 142 20 Czech Republic

Department of Nanotoxicology and Molecular Epidemiology Institute of Experimental Medicine CAS Vídeňská 1083 Prague 142 20 Czech Republic

Citace poskytuje Crossref.org