Screen-printed sensors with chemically deposited boron-doped diamond electrodes (BDDE) were modified with different types of gold nanoparticles (AuNPs) according to a new original procedure. Physically and electrochemically deposited AuNPs had various sizes and also nanoporous character. They also differ in shape and density of surface coverage. The developed sensors were characterized using scanning electron microscopy and Raman spectroscopy. Their electrochemical properties were studied using cyclic voltammetry and electrochemical impedance spectrometry of selected outer sphere ([Ru(NH3)6]Cl3) and inner sphere (K3[Fe(CN)6], dopamine) redox markers. The application possibilities of such novel screen-printed sensors with BDDE modified by AuNPs were verified in the analysis of the neurotransmitter dopamine. The best analytical performance was achieved using printed sensors modified with the smallest AuNPs. The achieved limit of detection values in nanomolar concentrations (2.5 nmol L-1) are much lower than those of unmodified electrodes, which confirms the significant catalytic effects of gold nanoparticles on the surface of the working electrode. Sensors with the best electrochemical properties were successfully applied in the analysis of a model solution and spiked urine samples.

Department of Analytical Chemistry Faculty of Chemical Technology University of Pardubice Studentská 573 532 10 Pardubice Czech Republic

Department of Graphic Arts Technology and Applied Photochemistry Faculty of Chemical and Food Technology Slovak University of Technology in Bratislava Radlinského 9 Bratislava 812 37 Slovak Republic

Faculty of Chemical Technology Institute of Environmental and Chemical Engineering University of Pardubice Studentská 573 532 10 Pardubice Czech Republic

Faculty of Electrical Engineering and Information Technology Institute of Electronics and Photonics Slovak University of Technology in Bratislava Ilkovičova 3 Bratislava 812 19 Slovak Republic

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