OBJECTIVES: Our objective was to compare the measurement of residual white blood cell (rWBC) and residual red blood cell (rRBC) counts in blood products using the XN Blood Bank mode and the laboratory standard operating procedures for manual counts. In addition, to compare the whole blood complete blood count (CBC) values of blood donors and the quality of blood products using the Sysmex XN analyser versus the XS-1000i analyser. MATERIALS AND METHODS: For blood donors, 190 samples from blood or apheresis donors were analysed on both the Sysmex XS-1000i and XN-1000 analysers and the mean values of six CBC parameters were compared: the white blood cell count (WBC), the red blood cell count (RBC), haemoglobin (HGB), haematocrit (HCT), the mean corpuscular volume (MCV), the platelet count (PLT). For blood products, 164 samples were collected: 13 Plasma products - whole blood, 9 Plasma products - apheresis, 36 RBC concentrates - whole blood, 30 PLT concentrates - buffy coats, 36 PLT concentrates - buffy coats - pooled and 55 PLT concentrates - apheresis. RESULTS: All CBC parameters of the blood donors tested showed similar performance, with excellent correlation coefficients (r) ranging from 0.821 to 0.995. The majority of the blood products did not have a quantifiable number of residual cells, meaning the number of rWBC and rRBC, if present, was below the limit of quantitation (LoQ) of the different methods. rWBC were detected by Blood Bank mode in Plasma products - whole blood with a mean rWBC of 0.012 × 109 /L and in PLT concentrates - buffy coats with a mean rWBC of 0.19 × 109 /L. The correlation coefficient in both analysers for all three parameters (HGB, HCT, RBC) in RBC concentrates - whole blood was excellent, ranging from 0.95 to 0.99. For platelet count, r ranged from 0.98 to 0.99. CONCLUSION: The XN-Series analyser, equipped with a Blood Bank mode, demonstrated reliable performance when used for blood donor evaluation, rWBC enumeration and measurement of end blood products.

Apheresis and Transfusion Departments Institute of Haematology and Blood Transfusion Prague Czech Republic

Haematology Department Sysmex Europe SE Norderstedt Germany

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US Food and Drug Administration Center for Biologics Evaluation and Research. Guidance for Industry: Pre-Storage Leukocyte Reduction of Whole Blood and Blood Components Intended for Transfusion. FDA; 2012 Available from: https://www.fda.gov/media/84460/download

Masse M, Naegelen C, Pellegrini N, Segier JM, Marpaux N, Beaujean F. Validation of a simple method to count very low white cell concentrations in filtered red cells or platelets. Transfusion. 1992;32:565-571.

Dzik S. Principles of counting low numbers of leukocytes in leukoreduced blood components. Transfus Med Rev. 1997;11:44-55.

Dzik S, Moroff G, Dumont L. A multicenter study evaluating three methods for counting residual WBCs in WBC-reduced blood components: Nageotte hemocytometry, flow cytometry, and microfluorometry. Transfusion. 2000;40:513-520.

Rebulla P, Dzik WH. Multicenter evaluation of methods for counting residual white cells in leukocyte-depleted red blood cells. The Biomedical Excellence for Safer Transfusion (BEST) working party of the international society of blood transfusion. Vox Sang. 1994;66:25-32.

Blanco RA, Cavagnetto C, Willmott L, et al. The use of a hematology analyzer with a new generation of software as an alternative to flow cytometry for enumerating residual white blood cells in blood components. Transfusion. 2020;60:155-164.

Quality assurance in haematology. WHO Document LAB/98.4 1998.

Zhang QY, Crist J, Ivers T. Performance comparison of two Sysmex hematology analyzers: the XN-550 and the XS-1000i. Sysmex J Int. 2018;28:1-12.

van der Meer PF, Dijkstra-Tiekstra MJ, Mahon A, de Wildt-Eggen J, BEST Collaborative. Counting platelets in platelet concentrates on hematology analyzers: a multicenter comparative study. Transfusion. 2009;49:81-90.

Arisawa F, Wakamoto S, Akino M, Ikuta K, Kino S. Evaluation of the Blood Bank mode software of Sysmex XN-1000 Hematology Analyzer for counting residual red blood cells and platelets in platelet concentrates, and residual white blood cells in leukocyte-reduced whole blood. Sysmex J Int. 2021;31:18-27.

Cavagnetto C, Alejo Blanco R, McKenna H, et al. Residual red cells in blood components: a multisite study of fully automated enumeration using a hematology analyzer. Transfusion. 2021;61:568-578.