First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics
Language English Country Netherlands Media print-electronic
Document type Case Reports, Journal Article
PubMed
38199682
DOI
10.1016/j.vprsr.2023.100961
PII: S2405-9390(23)00131-4
Knihovny.cz E-resources
- Keywords
- Anthelmintic treatment, Cattle, Resistance, Strongylid nematodes,
- MeSH
- Anthelmintics * therapeutic use MeSH
- Haemonchus * MeSH
- Ostertagia MeSH
- Pilot Projects MeSH
- Cattle MeSH
- Trichostrongyloidea * genetics MeSH
- Treatment Outcome MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Anthelmintics * MeSH
Parasitic diseases and mitigation of their effects play an important role in the health management of grazing livestock worldwide, with gastrointestinal strongylid nematodes being of prominent importance. These helminths typically occur in complex communities, often composed of species from numerous strongylid genera. Detecting the full diversity of strongylid species in non-invasively collected faecal samples is nearly impossible using conventional methods. In contrast, high-throughput amplicon sequencing (HTS) can effectively identify co-occurring species. During the four-year project, we collected and analysed faecal samples from beef cattle on >120 farms throughout the Czech Republic. Strongylids were the predominant nematodes, detected in 56% of the samples, but at a low level of infection. The apparent limitations in identifying strongylid taxa prompted this pilot study on a representative group of samples testing positive for strongylids using ITS-2 metabarcoding. The most widespread genera parasitizing Czech cattle were Ostertagia (O. ostertagi) and Oesophagostomum spp., followed by Trichostrongylus and Cooperia, while Bunostomum, Nematodirus and Chabertia were present only in a minority. As comparative material, 21 samples of cattle from the Danube Delta in Romania were used, which, in contrast, were dominated by Haemonchus placei. Finally, the effect of ivermectin treatment was tested at two Czech farms. After treatment with the anthelmintic, there was a shift in the strongylid communities, with a dominance of Cooperia and Ostertagia.
Czech Beef Breeders Association Těšnov 65 17 110 00 Prague Czech Republic
Department of Zoology Faculty of Science Charles University Viničná 7 128 44 Prague Czech Republic
State Veterinary Institute Jihlava Rantířovská 93 20 586 01 Jihlava Czech Republic
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