Dermoergasilus madagascarensis n. sp. is described from the gills of Paretroplus polyactis, an endemic cichlid fish in Madagascar, using a combined morphological (light microscopy and SEM) and molecular approach (partial 18S rDNA, 28S rDNA, and COI sequences). The new species is characterized mainly by possessing: (i) roughly pentagonal cephalosome; (ii) antennal endopodal segments covered with slightly inflated membrane; (iii) maxillule bearing 2 equally long outer setae and a minute inner seta; (iv) interpodal sternites of swimming legs ornamented with 3–4 rows of spinules; (v) genital segment and first abdominal somite both barrel-shaped; and (vi) a caudal ramus projecting into a digitiform process with inconspicuous terminal seta and bearing 3 terminal setae. The obtained DNA sequences of Malagasy species represent the first molecular data for species of Dermoergasilus. The 28S rDNA phylogeny showed the affiliation of D. madagascarensis n. sp. to Ergasilidae and its sister relationship with cosmopolitan Ergasilus sieboldi von Nordmann, 1832. The first checklist for all species of Dermoergasilus is provided.

Department of Animal Biology Faculty of Science University of Antananarivo BP 906 Antananarivo 101 Madagascar

Department of Botany and Zoology Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic

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Ahmed SM and Ali AH (2013) Serum proteins and leucocytes differential count in the common carp (

Ali AH and Adday TK (2019) Description of a new species of Dermoergasilus Ho & Do, 1982 (Copepoda: Ergasilidae) from the redbelly tilapia PubMed

Amado MAPM, Falavigna da Rocha CE, Piasecki W, Al-Daraji SA and Mhaisen FT (2001) Copepods of the family Ergasilidae (Poecilostomatoida) parasitic on fishes from Khor Al-Zubair lagoon, Iraq. Hydrobiologia 459, 213–221.

Baek SY, Jang KH, Choi EH, Ryu SH, Kim SK, Lee JH and Hwang UW (2016) DNA barcoding of metazoan zooplankton copepods from South Korea. PLoS ONE 11, 1–20. PubMed PMC

Barnard KH (1960) Isopoda parasitic on Madagascar fish. Institute Scientifique de Madagascar, Office de la Recherche Scientifique et Technique Outre-mers. Memoires, Série F 3, 93–95.

Benovics M, Kičinjaová ML and Šimková A (2017) The phylogenetic position of the enigmatic Balkan PubMed PMC

Benovics M, Koubková B, Civáňová K, Rahmouni I, Čermáková K and Šimková A (2021) Diversity and phylogeny of PubMed

Benstead JP, De Rham PH, Gattolliat JL, Gibon FM, Loiselle PV, Sartori M, Sparks JS and Stiassny ML (2003) Conserving Madagascar's freshwater biodiversity. BioScience 53, 1101–1111.

Benz GW (2006) A new genus and species of hyponeoid (Copepoda) from the olfactory sac of a gulper shark PubMed

Bucklin A, Frost B, Bradford-Grieve J, Allen L and Copley N (2003) Molecular systematic and phylogenetic assessment of 34 calanoid copepod species of the Calanidae and Clausocalanidae. Marine Biology 142, 333–343.

Bush AO, Lafferty KD, Lotz JM, Shostak AW (1997) Parasitology meets ecology on its own terms: Margolis PubMed

Byrnes TB (1986) Some ergasilids (Copepoda) parasitic on four species of Australian bream,

Cressey RF (1963) A new genus of copepods (Caligoida, Pandaridae) from a Thresher Shark in Madagascar. Cahiers ORSTOM, Série Océanographie 2, 285–297.

Cressey RF and Collette BB (1970) Copepods and needlefishes: a study in host-parasite relationships. Fishery Bulletin 68, 347–432.

Dippenaar SM, Mathibela RB and Bloomer P (2010) Cytochrome oxidase I sequences reveal possible cryptic diversity in the cosmopolitan symbiotic copepod PubMed

Dogiel VA and Akhmerov AK (1952) Parasitic Crustacea of fishes of the Amur. Uchenye Zapiski Leningradskogo Ordena Lenina Gosudarstvennogo Universiteta Imeni AA Zhdanova 141, 268–294.

El-Rashidy H and Boxshall GA (1999) Ergasilid copepods (Poecilostomatoida) from the gills of primitive Mugilidae (grey mullets). Systematic Parasitology 42, 161–168. PubMed

El-Rashidy H and Boxshall GA (2001) Biogeography and phylogeny of PubMed

Ergens R and Lom J (1970) Causative Agents of Fish Diseases. Prague: Academia.

Feng HL, Wang LX, Huang J, Jiang J, Tang D, Fang R and Su YB (2016) Complete mitochondrial genome of PubMed

Folmer O, Hoeh WR, Black MB and Vrijenhoek RC (1994) Conserved primers for PCR amplification of mitochondrial DNA from different invertebrate phyla. Molecular Marine Biology and Biotechnology 3, 294–299. PubMed

Fryer G (1968) The parasitic Crustacea of African freshwater fishes; their biology and distribution. Journal of Zoology 156, 45–95.

Gussev AV (1987) Phylum Arthropoda. Key to the Parasites of Freshwater Fishes of the Fauna of the USSR 3, 378–524.

Hassan M, Jones B and Lymbery AJ (2009) A new species of Dermoergasilus Ho & Do, 1982 (Copepoda: Ergasilidae) from freshwater fishes in the south-west of Western Australia. Systematic Parasitology 74, 143–148. PubMed

Ho JS and Do T (1982) Two species of Ergasilidae (Copepoda: Poecilostomatoida) parasitic on the gills of

Ho JS, Jayarajan P and Radhakrishnan S (1992) Copepods of the family Ergasilidae (Poecilostomatoida) parasitic on coastal fishes of Kerala, India. Journal of Natural History 26, 1227–1241.

Ho JS, Khamees NR and Mhaisen FT (1996) Ergasilid copepods (Poecilostomatoida) parasitic on the mullet Liza abu in Iraq, with the description of a new species of

Hoang DT, Chernomor O, von Haeseler A, Minh BQ and Vinh LS (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35, 518–522. PubMed PMC

Hua C (2020) Complete mitochondrial genome of

Huelsenbeck JP and Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics (Oxford, England) 17, 754–755. PubMed

Humes AG (1962)

Humes AG (1967)

Humes AG (1971) Cyclopoid copepods (Stellicomitidae) parasitic on sea stars from Madagascar and Eniwetok Atoll. The Journal of Parasitology 57, 1330–1343.

Humes AG (1974) Cyclopoid copepods (Lichomolgidae) from gorgonaceans in Madagascar. Proceedings of the Biological Society of Washington 87, 411–438.

Humes AG and Cressey RF (1958) A new family containing two new genera of cyclopoid copepods parasitic on starfishes. The Journal of Parasitology 44, 395–408. PubMed

Humes AG and Cressey RF (1959) A new family and genus of cyclopoid copepods parasitic on a holothurian. The Journal of Parasitology 45, 209–216. PubMed

Humes AG and Cressey RF (1961) Copépodes cyclopoides du genre

Humes AG and Frost BW (1964) New lichomolgid copepods (Cyclopoida) associated with alcyonarians and madreporarians in Madagascar. Cahiers ORSTOM Océanographie 6, 131–212.

Humes AG and Ho JS (1965) New species of the genus

Humes AG and Ho JS (1966) Cyclopoid copepods associated with the starfish

Humes AG and Ho JS (1967) New cyclopoid copepods associated with the coral

Humes AG, Cressey RF and Gooding RU (1958) A new cyclopoid copepod,

Kabata Z (1979) Parasitic Copepoda of British Fishes. London: Ray Society, 468 p., 199 pls.

Kabata Z (1992) Copepoda parasitic on Australian fishes, XV. Family Ergasilidae (Poecilostomatoida). Journal of Natural History 26, 47–66.

Kalyaanamoorthy S, Minh BQ, Wong TK, Von Haeseler A and Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14, 587–589. PubMed PMC

Katoh K and Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30, 772–780. PubMed PMC

Khamees NR and Mhaisen FT (1995) Two copepod crustaceans as additional species 681 to the parasitic fauna of fishes of Iraq. Basrah Journal of Science 13, 49–56.

Kvach Y, Tkachenko MY, Seifertová M and Ondračková M (2021) Insights into the diversity, distribution and phylogeny of three ergasilid copepods (Hexanauplia: Ergasilidae) in lentic water bodies of the Morava river basin, Czech Republic. Limnologica 91, 125922.

Laakmann S, Gerdts G, Erler R, Knebelsberger T, Martínez Arbizu P and Raupach MJ (2013) Comparison of molecular species identification for North Sea calanoid copepods (Crustacea) using proteome fingerprints and DNA sequences. Molecular Ecology Resources 13, 862–876. PubMed

Lima FS, Graca RJ, Fabrin TMC, Gasques LS, Prioli SMAP, Prioli AJ and Takemoto RM (2017) Phylogenetic position of copepods from Neotropical region in the Ergasilidae with markers Cytochrome C Oxidase I (COI) and rDNA 18S. (Unpublished).

Míč R, Řehulková E and Seifertová M (2023) Species of Ergasilus von Nordmann, 1832 (Copepoda: Ergasilidae) from cichlid fishes in Lake Tanganyika. Parasitology 150, 579–598. PubMed PMC

Miller MA, Pfeiffer W and Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In DOI

Nejat F, Benovics M, Řehulková E, Vukić J, Šanda R, Kaya C, Tarkan AS, Abdoli A, Aksu S and Šimková A (2023) Diversity, phylogeny and intraspecific variability of PubMed PMC

Nguyen L-T, Schmidt HA, von Haeseler A and Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32, 268–274. PubMed PMC

Oldewage WH and Van As JG (1988) Two new species of Ergasilidae (Copepoda: Poecilostomatoida) parasitic on

Oliveira MS, Corrêa LL, Adriano EA and Tavares-Dias M (2021) Integrative taxonomy of a new species of PubMed

Ondračková M, Fojtů J, Seifertová M, Kvach Y and Jurajda P (2019) Non-native parasitic copepod PubMed

Pleijel F, Jondelius U, Norlinder E, Nygren A, Oxelman B, Schander C, Sundberg P and Thollesson M (2008) Phylogenies without roots? A plea for the use of vouchers in molecular phylogenetic studies. Molecular Phylogenetics and Evolution 48, 369–371. PubMed

Rahmouni I, Řehulková E, Pariselle A, Rkhami OB and Šimková A (2017) Four new species of PubMed

Rambaut A (2012) FigTree v1.4.3 [Computer software]. Available at https://github.com/rambaut/figtree/releases, 5 February 2023.

Rambaut A, Drummond AJ, Xie D, Baele G and Suchard MA (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology 67, 901–904. PubMed PMC

Řehulková E, Benovics M and Šimková A (2020) Uncovering the diversity of monogeneans (Platyhelminthes) on endemic cypriniform fishes of the Balkan Peninsula: new species of PubMed PMC

Reshmi NVM and Kappalli S (2022). Genetic diversity of parasitic copepods from Kerala coast. (Unpublished).

Santacruz A, Morales-Serna FN, Leal-Cardín M, Barluenga M and Pérez-Ponce de León G (2020) PubMed

Šimková A, Řehulková E, Rasoloariniaina JR, Jorissen MW, Scholz T, Faltýnková A, Mašová Š and Vanhove MP (2019) Transmission of parasites from introduced tilapias: a new threat to endemic Malagasy ichthyofauna. Biological Invasions 21, 803–819.

Song Y, Wang GT, Yao WJ, Gao Q and Nie P (2008) Phylogeny of freshwater parasitic copepods in the Ergasilidae (Copepoda: Poecilostomatoida) based on 18S and 28S rDNA sequences. Parasitology Research 102, 299–306. PubMed

Su Y-B, Li-Xia W, Sheng-Chao K, Lu C and Rui F (2016) Complete mitochondrial genome of PubMed

Talavera G and Castresana J (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56, 564–577. PubMed

Tamura K, Stecher G and Kumar S (2021) MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38, 3022–3027. PubMed PMC

Trifinopoulos J, Nguyen L-T, von Haeseler A and Minh BQ (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44, W232–W235. PubMed PMC

Trilles J-P (1975) Les Cymothoidae (Isopoda, Flabellifera) des collections du Muséum National d'Histoire naturelle de Paris. III. Les Cymothoinae Schioedte et Meinert, 1884. Genre

Trilles J-P (1979) Les Cymothoidae (Isopoda, Flabellifera; parasites de poissons) du Rijksmuseum van Natuurlijke Historie te Leiden II. Afrique, Amerique et regions Indo-Ouest-Pacifique. Zoologische Mededelingen

Trilles J-P (2008) Some marine isopods from the Senckenberg Research Institute (Frankfurt am Main, Germany) (Crustacea, Isopoda: Cymothoidae, Aegidae, Corallanidae, Cirolanidae). Senckenbergiana Biologica 88, 21–28.

Vasquez AA, Bonnici BL, Kashian DR, Trejo-Martinez J, Miller CJ and Ram JL (2022) The biodiversity of freshwater Crustaceans revealed by taxonomy and mitochondrial DNA barcodes. Physiology Faculty Research Publications 3, 166–181.

Vences M, Wollenberg KC, Vieites DR and Lees DC (2009) Madagascar as a model region of species diversification. Trends in Ecology & Evolution 24, 456–464. PubMed

Yamaguti S (1939) Parasitic copepods from fishes of Japan. Part 5. Caligoida, III. Volumen Jubilare pro Professore Sadao Yoshida 2, 443–487, 33 pls.