The issue of bone volume loss is playing an increasing role in bone tissue engineering. Research has focused on studying the preparation and use of different types of human or xenogenic materials and their osteogenic properties. An alternative source for this purpose could be autologous extracted teeth. The simple preparation protocol, minimal immune response, and rapid organizing of the newly formed bone with optimal mechanical properties predispose autologous hard teeth tissues (HTTs) as a promising material suitable in the indication of augmentation of maxillary and mandible defects, comparable to other high-end augmentation materials. The aim of this study was to experimentally evaluate the osteogenic potential of ground native autologous HTTs prepared by different demineralization procedures, aimed at potentiating the osteoinductive and osteoconductive properties of their organic components. The results indicate that the most effective preparation process for HTT stimulation is the application of Cleanser for 10 min followed by exposure to 0.6 N HCl for 5 min with a wash in phosphate-buffered saline solution.

Department of Dentistry and Maxillofacial Surgery Faculty of Medicine Pavol Jozef Safarik University in Kosice Tr SNP 1 040 01 Kosice Slovakia

Department of Microbiology and Immunology University of Veterinary Medicine and Pharmacy in Kosice Komenskeho 73 041 81 Kosice Slovakia

Department of Stomatology and Maxillofacial Surgery Faculty of Medicine Pavol Jozef Safarik University in Kosice Tr SNP 1 040 11 Kosice Slovakia

Department of Stomatology Faculty of Medicine Masaryk University Kamenice 5 625 00 Brno Czech Republic

Institute of Materials Research Slovak Academy of Sciences ÚMV SAV Košice 040 01 Kosice Slovakia

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