Large herbivores are subject to handling and social stress in captivity. These may affect blood biochemical values, which motivated this research. Twelve healthy common eland (Taurotragus oryx) were monitored for 12 months. The animals were handled monthly, and blood samples were collected. Samples from every second month were analysed for 14 blood biochemical parameters. Temperament throughout the handling, as the summation of various behavioural responses, was calculated as a proxy of the stress generated during handling. Social behaviour was recorded each month, and the agonistic interactions were used to calculate the social rank, which was considered a proxy of social stress. Generalised Linear Mixed Models were designed to test the effects of temperament and social rank on the blood biochemical parameters while keeping sex, age, body condition, and body weight as covariates. The results show that the temperament during handling influences blood levels of albumin, alkaline phosphate, blood urea nitrogen, glucose, total bilirubin, and total protein; however, social rank has little influence, affecting just albumin. The ranges observed in the values of these biochemical parameters were still within their reference intervals, implying the absence of pathology or physiological problems during the study. The results suggest that blood biochemical values of physically restrained common eland should be carefully interpreted, even in animals already habituated to routine handling. On the contrary, social rank has low effects on the blood biochemical parameters.

Department of Animal Science and Food Processing Faculty of Tropical AgriSciences Czech University of Life Sciences Prague Kamýcká 129 Praha Suchdol 165 00 Czech Republic

Department of Ethology Institute of Animal Science Přátelství 815 Prague 10 Uhříněves 104 00 Czech Republic

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Ali BH, Al-Qarawi AA, Mousa HM. Stress associated with road transportation in desert sheep and goats, and the effect of pre-treatment with xylazine or sodium betaine. Res Vet Sci. 2006;80:343–348. doi: 10.1016/j.rvsc.2005.07.012. PubMed DOI

Altmann J. Observational study of behaviour: sampling methods. Behaviour. 1974;49:227–267. doi: 10.1163/156853974X00534. PubMed DOI

Audigé L, Wilson PR, Morris RS. A body condition score system and its use for farmed red deer hinds. New Zeal J Agr Res. 1998;41:545–553. doi: 10.1080/00288233.1998.9513337. DOI

Barroso FG, Alados CL, Boza J. Social hierarchy in the domestic goat: effect on food habits and production. App Ani Behav Sci. 2000;69:35–53. doi: 10.1016/S0168-1591(00)00113-1. PubMed DOI

Bica GS, Filho LCPM, Teixeira DL, de Sousa KT, Hötzel MJ. Time of grain supplementation and social dominance modify feeding behaviour of heifers in rotational grazing systems. Front Vet Sci. 2020;7:61. doi: 10.3389/fvets.2020.00061. PubMed DOI PMC

Brakes P. Sociality and wild animal welfare: future directions. Front Vet Sci. 2019;6:62. doi: 10.3389/fvets.2019.00062. PubMed DOI PMC

Ceacero F, Landete-Castillejos T, Bartošová J, García AJ, Bartoš M, Komárková M, Gallego L. Habituating to handling: factors affecting preorbital gland opening in red deer calves. J Anim Sci. 2014;92:4130–4136. doi: 10.2527/jas.2014-7716. PubMed DOI

Ceacero F, Gaspar-López E, García AJ, Landete-Castillejos T, Gallego L. Social rank affects the hematological profile in red deer hinds. Vet Rec. 2018;182:436. doi: 10.1136/vr.104629. PubMed DOI

Creel S, Creel NM, Monfort SL. Social stress and dominance. Nature. 1996;379:212. doi: 10.1016/S0169-5347(01)02227-3. DOI

Hemsworth PH. Human-animal interactions in livestock production. App Anim Behav Sci. 2003;81:185–198. doi: 10.1016/S0168-1591(02)00280-0. DOI

Hjarvard BM, Larsen ON, Juul-Madsen HR, Jørgensen E, Jensen KH. Social rank influences the distribution of blood leukocyte subsets in female growing pigs. Scan J Lab Anim Sci. 2009;36:309–320. doi: 10.23675/sjlas.v36i4.194. DOI

Hyvarinen H, Helle T, Nieminen M, Vayrynen Q, Vayrenen R. Some effects of handling reindeer during gatherings on the composition of their blood. Anim Prod. 1976;22:105–114.

Karns PD, Crichton VFJ. Effects of handling and physical restraint on blood parameters in Woodland Caribou. J Wildl Manag. 1978;42:904–908. doi: 10.2307/3800782. DOI

Kock N, Lynn G. Lateral collinearity and misleading results in variance-based SEM: an illustration and recommendations. J Assoc Inf Sys. 2012;13:546–580.

Koolhaas JM, Korte SM, De Boer SF, Van Der Vegt BJ, Van Reenen CG, Hopster H, De Jong IC, Ruis MAW, Blokhuis HJ. Coping styles in animals: current status in behaviour and stress-physiology. Neurosci Biobehav R. 1999;23:925–935. doi: 10.1016/S0149-7634(99)00026-3. PubMed DOI

Kumar P, Ahmed MA, Abubakar AA, Hayat MN, Kaka U, Ajat M, Goh YM, Sazili AQ. Improving animal welfare status and meat quality through assessment of stress biomarkers: a critical review. Meat Sci. 2022;197:109048. doi: 10.1016/j.meatsci.2022.109048. PubMed DOI

Mahre MB, Wahid H, Rosnina Y, Jesse FFA. Assessment of temperament in Rusa timorensis and its relationship to stress. J Anim Sci. 2015;93:1276–1283. doi: 10.2527/jas.2014-8080. PubMed DOI

Marco I, Lavin S. Effect of the method of capture on the haematology and blood chemistry of red deer (Cervus elaphus) Res Vet Sci. 1999;66:81–84. doi: 10.1053/rvsc.1998.0248. PubMed DOI

Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J. 2004;168:28–40. doi: 10.1016/S1090-0233(03)00119-9. PubMed DOI

Musa AS, Needham T, Kotrba R, Neradilova S, Ganswindt A, Ceacero F. Habituation of common eland (Taurotragus oryx) to intensive routine handling, and the effect of immunocastration thereon. App Anim Behav Sci. 2021;237:105294. doi: 10.1016/j.applanim.2021.105294. DOI

Parham JT, Blevins SR, Tanner AE, Wahlberg ML, Swecker WS, Jr, Lewis RM. Subjective methods of quantifying temperament in heifers are indicative of physiological stress. App Anim Behav Sci. 2021;234:105197. doi: 10.1016/j.applanim.2020.105197. DOI

Pospisil J, Kase F, Vahala J, Mouchova I. Basic hematological values in antelopes –IV. A comparison and concluding remarks. Comp Biochem Physiol. 1984;78:815–821. doi: 10.1016/0300-9629(84)90639-x. PubMed DOI

Schmid VS, de Vries H. Finding a dominance order most consistent with a linear hierarchy: an improved algorithm for the I&SI method. Anim Behav. 2013;86:1097–1105. doi: 10.1016/j.anbehav.2013.08.019. DOI

Tuchscherer M, Puppe B, Tuchscherer A, Kanitz E. Effects of social status after mixing on immune, metabolic, and endocrine responses in pigs. Physiol Behav. 1998;64:353–360. doi: 10.1016/S0031-9384(98)00084-5. PubMed DOI

Vahala J, Spala P, Kase F. Biochemical blood serum values of healthy antelopes kept in the East-Bohemian zoological garden at Dvůr Králové Nad Labem – I. The Tragelaphinae, the Hippotraginae and the Aepycerotinae. Acta Vet Brno. 1989;58:17–29. doi: 10.2754/avb198958010017. DOI

Wirtu G, Pope CE, Vaccaro J, Sarrat E, Cole A, Godke RA, Dresser BL. Dominance hierarchy in a herd of female eland antelope (Taurotragus oryx) in captivity. Zoo Biol. 2004;23:323–333. doi: 10.1002/zoo.20003. DOI

Woodford MH. Wild relatives of domestic livestock & some suggestions for new domesticants. In: Scherf BD, editor. World Watch List for Domestic Animal Diversity. 3. Rome, Italy: Food and Agriculture Organization of The United Nations; 2000.

ZIMS (2022) Species360 Zoological Information. Expected Test Results for Taurotragus oryx. http://zims.Species360.org (Accessed 25 April, 2022)