Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Arabidopsis thaliana, BiFC, Protein-protein Interactions, Protoplast,
- MeSH
- Agrobacterium genetics metabolism MeSH
- Arabidopsis * metabolism genetics MeSH
- Microscopy, Fluorescence methods MeSH
- Plant Leaves * metabolism genetics MeSH
- Luminescent Proteins metabolism genetics MeSH
- Protein Interaction Mapping methods MeSH
- Arabidopsis Proteins metabolism genetics MeSH
- Protoplasts * metabolism MeSH
- Nicotiana metabolism genetics MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Luminescent Proteins MeSH
- Arabidopsis Proteins MeSH
Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented.
Central European Institute of Technology Masaryk University Brno Czech Republic
Institute of Science and Technology Austria Klosterneuburg Austria
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