Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Arabidopsis thaliana, BiFC, Protein-protein Interactions, Protoplast,
- MeSH
- Agrobacterium genetika metabolismus MeSH
- Arabidopsis * metabolismus genetika MeSH
- fluorescenční mikroskopie metody MeSH
- listy rostlin * metabolismus genetika MeSH
- luminescentní proteiny metabolismus genetika MeSH
- mapování interakce mezi proteiny metody MeSH
- proteiny huseníčku metabolismus genetika MeSH
- protoplasty * metabolismus MeSH
- tabák metabolismus genetika MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- luminescentní proteiny MeSH
- proteiny huseníčku MeSH
Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented.
Central European Institute of Technology Masaryk University Brno Czech Republic
Institute of Science and Technology Austria Klosterneuburg Austria
Zobrazit více v PubMed
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