Qualitative scoring methods are tools for rapid freshwater health assessments. Total score is often calculated as the sum or mean of the values of the species involved, with minor nuances in interpretation, but with significant implications. We used the Dragonfly Biotic Index (DBI) calculated on Central European odonate species to demonstrate these implications. Each species within a community has a score ranging from 0 (widespread generalists) to 9 (sensitive specialists). A total score is calculated as the sum of the scores of all species (DBIsum) or is calculated by dividing by species richness (DBImean). Despite this duality, there has been little debate on either approach. Using simulated scenarios (high vs low richness, presence or absence of high- or low-scoring species), we tested the implications of DBIsum and DBImean and suggested a total score calculation for conservation prioritization based on permutation. This algorithm shows the percentile of a community compared to a set of randomly assembled communities of the same species richness. We also present the 'dragDBI' package for the statistical software R, a tool for more automated DBI-based environmental health assessments. Our permutational calculation is applicable to other macroinvertebrate-based scoring methods, such as the Biological Monitoring Working Party and the Average Score Per Taxon.

Department of Biology and Ecology Faculty of Science University of Ostrava Chittussiho 10 710 00 Ostrava Czech Republic

Department of Zoology Faculty of Science Palacký University Olomouc Czech Republic

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