Citrus reticulata L leaves are one of the main post-harvest byproduct, containing bioactive compounds, that are usually undervalued. This work describes the development of a biorefinery process based on the application of supercritical CO2 (SC-CO2) followed by ultrasonic-assisted extraction (UAE) combined with Natural Deep Eutectic Solvents (NaDES) to extract bioactive terpenoids and phenolic compounds from these leaves. Extraction temperature and pressure of SC-CO2 were optimized, obtaining the highest bioactive terpenoids content using 200 bar at 60 °C. A Box-Behnken experimental design showed that 57% of water in NaDES composed of Choline Chloride and Glycerol (1:2) as extraction solvent at 25 °C for 50 min were the optimal UAE-NaDES extraction conditions to obtain the highest bioactive phenolic content from the residue of the optimal SC-CO2 extraction. The optimum extract presented the highest bioactivity and polyphenol content determined by LC-DAD-MS compared with extracts obtained using only water or NaDES as solvent.

High Pressure Laboratory Food Chemistry Research Group Departamento de Química Facultad de Ciencias Universidad Nacional de Colombia Carrera 30 No 45 03 111321 Bogotá D C Colombia

Laboratory of Foodomics Institute of Food Science Research CIAL CSIC Nicolás Cabrera 9 28049 Madrid Spain

Laboratory of Foodomics Institute of Food Science Research CIAL CSIC Nicolás Cabrera 9 28049 Madrid Spain; Universidad de Alcalá Departamento de Química Analítica Química Física e Ingeniería Química Ctra Madrid Barcelona Km 33 600 28871 Alcalá de Henares Madrid Spain

Research Institute of Brewing and Malting Mostecká 7 614 00 Brno Czech Republic

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