The excellent electroconductive properties of polypyrrole (PPy) predetermine its use as cell-instructive or biosensing biomaterial. PPy has potential for application in a physiological environment, and here the cytotoxicity, antioxidant capacity, modulation of neutrophil oxidative burst, antibacterial activity of colloidal PPy stabilized with polyvinylpyrrolidone (PVP), and long-term stability under physiological conditions are reported. Besides, long-term stability under physiological conditions is also provided. The formation of PPy/PVP colloid was proven by UV-Vis and IR spectroscopy, and its morphology and particle size were detected. The results of cytotoxicity studies show that the safe PPy concentration in colloidal particles for biomedical usage is ≤ 200 µg mL-1. In these concentrations, the PPy/PVP also exhibits excellent antioxidant properties and modulates the immune response. The minimal inhibitory concentration of 234 µg mL-1 was determined against Staphylococcus aureus and Escherichia coli, respectively. The low cytotoxicity and bioactivity of the colloidal PPy/PVP demonstrate their long-term potential for a variety of applications.

Centre of Polymer Systems Tomas Bata University in Zlin nám T G Masaryka 5678 76001 Zlin Czech Republic

Centre of Polymer Systems Tomas Bata University in Zlin nám T G Masaryka 5678 76001 Zlin Czech Republic; Faculty of Technology Tomas Bata University in Zlin Vavrečkova 5669 76001 Zlin Czech Republic

Institute of Biophysics of the Czech Academy of Sciences Královopolská 135 61200 Brno Czech Republic

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Method for in situ polypyrrole coating, and the example of its use for functionalization of polyurethane anisotropic electrospun mats