Analysis of 594 isolates of Escherichia coli sequence type (ST)131 and its single locus variants carrying carbapenemase genes from 17 European Union/European Economic Area countries revealed acquisition of 18 carbapenemase variants, mainly in ST131 clades A and C. Most frequent were bla OXA-244 (n = 230) and bla OXA-48 (n = 224), detected in 14 and 12 countries, respectively. Isolates carrying bla OXA-244 have increased rapidly since 2021. The increasing detection of carbapenemase genes in the E. coli high-risk lineage ST131 is a public health concern.

Associated French National Reference Centre for Antibiotic Resistance Carbapenemase Producing Enterobacteriaceae Le Kremlin Bicêtre France

Austrian National Reference Centre for Antimicrobial Resistance Ordensklinikum Linz Elisabethinen Linz Austria

Centre for Infectious Disease Control National Institute for Public Health and the Environment Bilthoven the Netherlands

Department of Infectious Disease Epidemiology Robert Koch Institute Berlin Germany

Department of Microbiology 3rd Faculty of Medicine Charles University University Hospital Kralovske Vinohrady and National Institute of Public Health Prague Czechia

Department of Microbiology Faculty of Medicine University Hospital in Pilsen Charles University Pilsen Czechia

European Centre for Disease Prevention and Control Stockholm Sweden

Finnish Institute for Health and Welfare Helsinki Finland

Galway Reference Laboratory Service Galway University Hospital Galway Ireland

German National Reference Centre for Multidrug resistant Gram negative Bacteria Department of Medical Microbiology Ruhr University Bochum Bochum Germany

Institute of Microbiology and Immunology Faculty of Medicine University of Ljubljana Ljubljana Slovenia

National Centre for Public Health and Pharmacy Budapest Hungary

National Laboratory of Health Environment and Food Centre for Medical Microbiology Maribor Slovenia

National Microbiology Reference Laboratory of Latvia Laboratory Latvian Centre of Infectious Diseases Laboratory Service Riga East University Hospital Riga Latvia

National Public Health Surveillance Laboratory Vilnius Lithuania

National Reference Centre for Antimicrobic Resistant Gram Negative Bacilli Laboratory of Microbiology CHU UCL Namur Yvoir Belgium

National Reference Laboratory for Antibiotics National Institute of Public Health Prague Czechia

National Reference Laboratory for Antimicrobial Resistance Department of Bacteria Parasites and Fungi Statens Serum Institut Copenhagen Denmark

National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections Department of Infectious Diseases National Institute of Health Dr Ricardo Jorge Lisbon Portugal

Norwegian National Advisory Unit on Detection of Antimicrobial Resistance Department of Microbiology and Infection Control University Hospital of North Norway Tromsø Norway

Public Health Agency of Sweden Solna Sweden

School of Medicine University of Galway Galway Ireland

Service Bactériologie Mycologie Antibiorésistance Hygiène Hospitalière Département de Microbiologie Laboratoire National de Santé Dudelange Luxembourg

Team Resist UMR1184 Immunology of Viral Auto Immune Hematological and Bacterial diseases INSERM Université Paris Saclay CEA IHU Prometheus Faculty of Medicine Le Kremlin Bicêtre France

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