Dexamethasone Acetate-Loaded PLGA Nanospheres Targeting Liver Macrophages
Language English Country Germany Media print-electronic
Document type Journal Article
Grant support
CZ.02.01.01/00/22_008/0004607
New Technologies for Translational Research in Pharmaceutical Sciences/NETPHARM, co-funded by the European Union
Project No
Long Term Organization Development Plan 1011 Healthcare Challenges of WMD II of the Military Faculty of Medicine, Hradec Kralove, University of Defence, Czech Republic
DZRO-FVZ22-ZHN II
Long Term Organization Development Plan 1011 Healthcare Challenges of WMD II of the Military Faculty of Medicine, Hradec Kralove, University of Defence, Czech Republic
SVV 260 661
Ministerstvo Školství, Mládeže a Tělovýchovy
PubMed
39611304
PubMed Central
PMC11827543
DOI
10.1002/mabi.202400411
Knihovny.cz E-resources
- Keywords
- PLGA nanospheres, biodegradable nanoparticles, glucocorticoids, liver inflammation, macrophages,
- MeSH
- Anti-Inflammatory Agents pharmacology chemistry MeSH
- Dexamethasone * pharmacology chemistry analogs & derivatives MeSH
- Liver * drug effects metabolism MeSH
- Polylactic Acid-Polyglycolic Acid Copolymer * chemistry MeSH
- Macrophages * drug effects metabolism MeSH
- Mice MeSH
- Nanospheres * chemistry MeSH
- Drug Carriers chemistry pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Inflammatory Agents MeSH
- Dexamethasone * MeSH
- dexamethasone acetate MeSH Browser
- Polylactic Acid-Polyglycolic Acid Copolymer * MeSH
- Drug Carriers MeSH
Glucocorticoids are potent anti-inflammatory drugs, although their use is associated with severe side effects. Loading glucocorticoids into suitable nanocarriers can significantly reduce these undesirable effects. Macrophages play a crucial role in inflammation, making them strategic targets for glucocorticoid-loaded nanocarriers. The main objective of this study is to develop a glucocorticoid-loaded PLGA nanocarrier specifically targeting liver macrophages, thereby enabling the localized release of glucocorticoids at the site of inflammation. Dexamethasone acetate (DA)-loaded PLGA nanospheres designed for passive macrophage targeting are synthesized using the nanoprecipitation method. Two types of PLGA NSs in the size range of 100-300 nm are prepared, achieving a DA-loading efficiency of 19 %. Sustained DA release from nanospheres over 3 days is demonstrated. Flow cytometry analysis using murine bone marrow-derived macrophages demonstrates the efficient internalization of fluorescent dye-labeled PLGA nanospheres, particularly into pro-inflammatory macrophages. Significant down-regulation in pro-inflammatory cytokine genes mRNA is observed without apparent cytotoxicity after treatment with DA-loaded PLGA nanospheres. Subsequent experiments in mice confirm liver macrophage-specific nanospheres accumulation following intravenous administration using in vivo imaging, flow cytometry, and fluorescence microscopy. Taken together, the data show that the DA-loaded PLGA nanospheres are a promising drug-delivery system for the treatment of inflammatory liver diseases.
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