Voltammetric analysis of glycoproteins containing sialylated and neutral glycans at pyrolytic graphite electrode
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
39637451
DOI
10.1016/j.bioelechem.2024.108851
PII: S1567-5394(24)00213-5
Knihovny.cz E-resources
- Keywords
- Chemical modification of carbohydrates, Electrochemical analysis, N-acetyl neuraminic acid, Osmium(VI) complexes, Sialoglycoproteins,
- MeSH
- Electrochemical Techniques * methods MeSH
- Electrodes MeSH
- Glycoproteins * chemistry analysis MeSH
- Graphite * chemistry MeSH
- N-Acetylneuraminic Acid * chemistry analysis MeSH
- Polysaccharides * chemistry analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glycoproteins * MeSH
- Graphite * MeSH
- N-Acetylneuraminic Acid * MeSH
- Polysaccharides * MeSH
Recently, it was described that neutral glycans can be distinguished from those containing sialic acid at the mercury electrode after modification with osmium(VI) N,N,N',N'-tetramethylethylenediamine (Os(VI)tem). Our work shows the possibility of studying glycans and glycoproteins at pyrolytic graphite electrodes depending on thepresence of sialic acid. Short glycans, glycans released from glycoproteins, and glycoproteins themselves yielded similar voltammetric responses after their modification by Os(VI)tem. Os(VI)tem modified glycans and glycoproteins produced acouple of cathodic and anodic peaks. Changing peak heights and potentials of glycans and glycoproteins pointed out the presence of sialic acid. These findings could be utilized to improve glycoprotein sensing by chemical modification.
References provided by Crossref.org
