Recently, it was described that neutral glycans can be distinguished from those containing sialic acid at the mercury electrode after modification with osmium(VI) N,N,N',N'-tetramethylethylenediamine (Os(VI)tem). Our work shows the possibility of studying glycans and glycoproteins at pyrolytic graphite electrodes depending on thepresence of sialic acid. Short glycans, glycans released from glycoproteins, and glycoproteins themselves yielded similar voltammetric responses after their modification by Os(VI)tem. Os(VI)tem modified glycans and glycoproteins produced acouple of cathodic and anodic peaks. Changing peak heights and potentials of glycans and glycoproteins pointed out the presence of sialic acid. These findings could be utilized to improve glycoprotein sensing by chemical modification.

Department of Biophysical Chemistry and Molecular Oncology Institute of Biophysics CAS v v i Královopolská 135 612 00 Brno Czech Republic

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