Complexity of synovial fluid-derived monocyte-macrophage-lineage cells in knee osteoarthritis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
39661512
DOI
10.1016/j.celrep.2024.115011
PII: S2211-1247(24)01362-7
Knihovny.cz E-resources
- Keywords
- CP: Immunology, early-stage OA, inactive myeloid dendritic cells, joint immune microenvironment, knee osteoarthritis effusion, knee synovitis, monocytes, synovial fluid cells, synovial fluid-derived macrophages, synovial inflammation, synovial tissue,
- MeSH
- Osteoarthritis, Knee * pathology metabolism immunology MeSH
- Cell Lineage MeSH
- Dendritic Cells * metabolism immunology MeSH
- Middle Aged MeSH
- Humans MeSH
- Macrophages * metabolism immunology MeSH
- Monocytes * metabolism immunology MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Synovial Fluid * metabolism immunology MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Synovial fluid (SF)-derived monocyte-macrophage (MON-Mϕ)-lineage cells in knee osteoarthritis (KOA) remain poorly understood. We analyzed SF samples from 420 patients with KOA with effusion. The MON-Mϕ cells accounted for 47.4% (median; range 7.1%-94.4%) of CD45+ cells and consisted of four subpopulations that correlated with the distribution and activation of other immune cells. The most abundant subpopulation was that of inactive CD11b+CD14-CD16- myeloid dendritic cells (mDCs; cDC2), which exhibited low cytokine production, low T lymphocyte stimulation, and high migratory ability. Other major subpopulations included CD11b+CD14+CD16- monocyte-like cells and CD11b+CD14+CD16+ macrophages, which share a similar transcriptomic profile. A subpopulation of CD11b-CD14-CD16- mDCs (cDC1) was less common. A higher proportion of CD11b+CD14-CD16- mDCs was linked to early-stage KOA and mild joint pain. Dendritic cells were rarely present in KOA synovium. This study revealed the considerable complexity of SF-derived MON-Mϕ subpopulations and highlighted the role of inactive mDCs in KOA.
References provided by Crossref.org
GEO
GSE279915
