Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
39725205
DOI
10.1016/j.envpol.2024.125579
PII: S0269-7491(24)02296-6
Knihovny.cz E-zdroje
- Klíčová slova
- Effect-directed analysis, Endocrine disrupting compounds, Glucocorticoid activity, In vitro, Indoor dust, Orthogonal fractionation,
- MeSH
- chemická frakcionace MeSH
- endokrinní disruptory analýza MeSH
- glukokortikoidy * analýza MeSH
- klobetasol MeSH
- látky znečišťující vzduch analýza MeSH
- lidé MeSH
- mometason furoát MeSH
- monitorování životního prostředí metody MeSH
- prach * analýza MeSH
- receptory glukokortikoidů metabolismus MeSH
- znečištění vzduchu ve vnitřním prostředí * analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- endokrinní disruptory MeSH
- glukokortikoidy * MeSH
- klobetasol MeSH
- látky znečišťující vzduch MeSH
- mometason furoát MeSH
- prach * MeSH
- receptory glukokortikoidů MeSH
Indoor dust contains various endocrine-disrupting contaminants, yet the effect drivers of observed glucocorticoid activity are completely unknown. This study conducted an effect-directed analysis using orthogonal fractionation to identify effect drivers of glucocorticoid activity in indoor dust. After the detection of bioactivity using a human cell line stably transfected with a reporter gene, the sample underwent parallel HPLC fractionations with octadecyl, pentafluorophenyl, and aminopropyl columns to obtain orthogonal fractions. The bioassays were utilized to screen the fractions and guide efforts towards prioritization of the bioactive chemicals using targeted and non-targeted analysis with LC-HRMS. The glucocorticoid activity of the identified potential candidates was confirmed by their testing in the same bioassay. To assess their contribution to the detected mixture effects, we calculated their relative potencies. This approach led to the identification of two pharmaceuticals, clobetasol propionate and mometasone furoate, at concentrations ranging from ng to μg per gram of dust, which together accounted for up to 77% of the observed glucocorticoid activity. This is the first report documenting the effect drivers of glucocorticoid receptor agonism in indoor dust; however, together with previous studies of various environmental samples, it documents that in cases when glucocorticoid receptor-agonistic activity is detected, drugs should be considered as likely relevant contaminants. The discovery of potent drugs in household dust highlights concerns for individuals exposed within domestic environments and emphasizes the need to consider pharmaceuticals as relevant contributors to indoor contamination.
Helmholtz Centre for Environmental Research UFZ Department of Exposure Science 04318 Leipzig Germany
RECETOX Faculty of Science Masaryk University Kotlarska 2 611 37 Brno Czech Republic
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