Overcoming Nanosilver Resistance: Resensitizing Bacteria and Targeting Evolutionary Mechanisms
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
39739341
DOI
10.1021/acsnano.4c15607
Knihovny.cz E-zdroje
- Klíčová slova
- envelope stress, evolutionary transition, nanoresistance, resensitization, silver nanoparticle,
- MeSH
- antibakteriální látky * farmakologie chemie MeSH
- Bacteria účinky léků metabolismus MeSH
- bakteriální léková rezistence účinky léků MeSH
- Escherichia coli účinky léků metabolismus MeSH
- kovové nanočástice * chemie MeSH
- mikrobiální testy citlivosti * MeSH
- stříbro * chemie farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky * MeSH
- stříbro * MeSH
The rapid spread of antimicrobial resistance poses a critical threat to global health and the environment. Antimicrobial nanomaterials, including silver nanoparticles (AgNPs), are being explored as innovative solutions; however, the emergence of nanoresistance challenges their effectiveness. Understanding resistance mechanisms is essential for developing antievolutionary strategies. AgNPs exhibit diverse resistance mechanisms, and our findings reveal a dynamic transition between these mechanisms: from flagellin-mediated AgNP precipitation (state I) to activation of the copper efflux pump (CusCFBA) system (state II). We designed targeted physicochemical interventions to counteract these mechanisms. Energy supply blocking was effective for state I, while for state II, neutralizing intracellular acidic pH significantly reduced resistance. These strategies reduced nanoresistance/tolerance by up to 10,000-fold. Additionally, resistance evolution can be completely halted by disrupting the energy supply using carbonyl cyanide 3-chlorophenylhydrazone and overactivating sigma E, one of the key envelope stress regulators that govern resistance transitions. Our findings provide practical strategies to overcome nanoresistance, offering a groundbreaking approach to enhance nanoantimicrobials' efficacy in medical therapies and combat resistance evolution.
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