Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
39746290
DOI
10.1016/j.talanta.2024.127456
PII: S0039-9140(24)01838-1
Knihovny.cz E-resources
- Keywords
- Capillary electrophoresis, Fluorescence, Glycan, Labeling, Mass spectrometry, Rhodamine B hydrazide,
- MeSH
- Electrophoresis, Capillary * methods MeSH
- Fluorescent Dyes * chemistry MeSH
- Spectrometry, Fluorescence methods MeSH
- Mass Spectrometry methods MeSH
- Lasers MeSH
- Oligosaccharides * chemistry analysis MeSH
- Polysaccharides * analysis chemistry MeSH
- Rhodamines * chemistry MeSH
- Cattle MeSH
- Trastuzumab chemistry analysis MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fluorescent Dyes * MeSH
- Oligosaccharides * MeSH
- Polysaccharides * MeSH
- rhodamine B MeSH Browser
- Rhodamines * MeSH
- Trastuzumab MeSH
In this work, we present the synthesis and application of fluorescent rhodamine B hydrazide for the derivatization of simple oligosaccharides and complex glycans using a hydrazone formation chemistry approach. The labeling conditions and the experimental setup of CE/LIF were optimized by analyzing oligosaccharide standards. The CE/LIF separations were performed in polybrene-coated capillaries eliminating the need for the purification step after derivatization. The addition of methanol to the background electrolyte significantly increased the LIF detection sensitivity reaching the limits of detection in the attomole range. The resolution of carbohydrate samples was improved by using long (98 cm) capillaries and polymer additives (polybrene). The developed method was applied for CE/LIF and CE-MS analysis of N-linked glycans released from bovine ribonuclease B and the therapeutic monoclonal antibody of trastuzumab.
References provided by Crossref.org
