Proteomic analysis of chick embryonic heart in experimental hypoxia
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
39933632
DOI
10.1016/j.ydbio.2025.02.006
PII: S0012-1606(25)00036-3
Knihovny.cz E-resources
- Keywords
- Chick embryo, Glycolysis, Heart, Hypoxia, Metabolism, Proteomics,
- MeSH
- Apoptosis MeSH
- Glycolysis MeSH
- Hypoxia * metabolism embryology MeSH
- Chick Embryo MeSH
- Myocardium * metabolism MeSH
- Proteome * metabolism MeSH
- Proteomics * methods MeSH
- Heart * embryology MeSH
- Animals MeSH
- Check Tag
- Chick Embryo MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Proteome * MeSH
Investigating prenatal hypoxia is difficult in mammals, as there are confounding factors stemming from maternal adaptations and compensatory mechanisms. We have thus established an avian model of hypoxic incubation (starting after 2 days of normoxia, 15% O2, normobaric, until the time of sampling at embryonic day 8) to study embryonic reactions to low oxygen concentration. Our previous studies have shown increased vascularization, oedema, and ventricular wall thinning preceding the lethality at mid-gestation. Analysis of the cardiac proteome after 6 days of hypoxic incubation showed strong upregulation of enzymes involved in anaerobic glycolysis as well as an increase in apoptosis-related proteins, cell adhesion proteins, and secretory activity.
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