Proteomic analysis of chick embryonic heart in experimental hypoxia
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
39933632
DOI
10.1016/j.ydbio.2025.02.006
PII: S0012-1606(25)00036-3
Knihovny.cz E-zdroje
- Klíčová slova
- Chick embryo, Glycolysis, Heart, Hypoxia, Metabolism, Proteomics,
- MeSH
- apoptóza MeSH
- glykolýza MeSH
- hypoxie * metabolismus embryologie MeSH
- kuřecí embryo MeSH
- myokard * metabolismus MeSH
- proteom * metabolismus MeSH
- proteomika * metody MeSH
- srdce * embryologie MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- proteom * MeSH
Investigating prenatal hypoxia is difficult in mammals, as there are confounding factors stemming from maternal adaptations and compensatory mechanisms. We have thus established an avian model of hypoxic incubation (starting after 2 days of normoxia, 15% O2, normobaric, until the time of sampling at embryonic day 8) to study embryonic reactions to low oxygen concentration. Our previous studies have shown increased vascularization, oedema, and ventricular wall thinning preceding the lethality at mid-gestation. Analysis of the cardiac proteome after 6 days of hypoxic incubation showed strong upregulation of enzymes involved in anaerobic glycolysis as well as an increase in apoptosis-related proteins, cell adhesion proteins, and secretory activity.
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