This study focused on the development of a suitable synthetic polymer scaffold for bone tissue engineering applications within the biomedical field. The investigation centered on electrospun polyvinylidene fluoride (PVDF) nanofibers, examining their intrinsic properties and biocompatibility with the human osteosarcoma cell line Saos-2. The influence of oxygen, argon, or combined plasma treatment on the scaffold's characteristics was explored. A comprehensive design strategy is outlined for the fabrication of a suitable PVDF scaffold, encompassing the optimization of electrospinning parameters with rotating collector and plasma etching conditions to facilitate a subsequent osteoblast cell culture. The proposed methodology involves the fabrication of the PVDF tissue scaffold, followed by a rigorous series of fundamental analyses encompassing the structural integrity, chemical composition, wettability, crystalline phase content, and cell adhesion properties.

Central European Institute of Technology Purkyňova 656 123 61200 Brno Czech Republic

Department of Ceramics and Polymers Faculty of Mechanical Engineering Brno University of Technology Technická 2896 2 61600 Brno Czech Republic

Department of Microelectronics Faculty of Electrical Engineering and Communication Brno University of Technology Technická 3058 10 61600 Brno Czech Republic

Department of Physics Faculty of Electrical Engineering and Communication Brno University of Technology Technická 2848 8 61600 Brno Czech Republic

Directorate of Research Development and Innovation Management Technical University of Cluj Napoca Constantin Daicoviciu Street No 15 400020 Cluj Napoca Cluj County Romania

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