Artificial conditions limit the ability of laboratory studies to describe the complex effects of polluted environments on aquatic life. This study aimed to evaluate the impacts of treated wastewater discharge on the survival, growth, and sex ratio balance of the population of brown trout (Salmo trutta m. fario) in situ. Five floating incubators with 1000 eggs each were placed in the upstream reference and treated wastewater-affected sites in the Czech Republic for approximately three months. The hatched fish were grown in a natural environment for nearly one year. Water quality, including nutrients, temperature, pharmaceutical and personal care products, biological effects by bioassays and fish mortality, metabolic rate, and growth, were measured regularly. Up to 72 pharmaceutical and personal care products (7400-23000 ng/sampler) were detected in the passive samplers deployed downstream of the sewage treatment plant effluent. In vitro bioassays of the sampler extracts indicated elevated oestrogenic effects, transthyretin binding inhibition, and aryl hydrocarbon-mediated and androgenic potencies, showing endocrine-disrupting potential at the polluted site. The cumulated mortality of brown trout in the exposed group (9.67%) was significantly higher (p < 0.05) than in the control group (5.16%). In addition, the body size, growth, and metabolic rate of exposed fish were significantly lower (p < 0.05). The sex ratio of brown trout in the effluent-affected stretch was imbalanced, and sterile individuals were detected after several months of natural development in the stream. The observed effects of treated wastewater on the early developmental stages of aquatic wildlife could be connected to the development and readiness of adult individuals and, consequently, to the sustainability of freshwater ecosystems. Applying the hatching apparatus used in fishery practices, followed by comparing mortality, development, and sex with reference localities, seems to be a promising biomonitoring approach that can indicate hotspots for in-depth investigation and risk assessment.

Czech University of Life Sciences Prague Department of Zoology and Fisheries Praha 6 Kamýcká 129 165 00 Suchdol Czech Republic

RECETOX Faculty of Science Masaryk University Kotlarska 2 61137 Brno Czech Republic

University of South Bohemia in České Budějovice Faculty of Fisheries and Protection of Waters South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Zátiší 728 2 389 25 Vodňany Czech Republic

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