We provide a supplemental description of the type species for Cryptogonimus Osborn, 1903 (Digenea: Cryptogonimidae), Cryptogonimus chili Osborn, 1903, based on newly-collected, heat-killed, formalin-fixed specimens infecting rock bass, Ambloplites rupestris (Rafinesque), and smallmouth bass, Micropterus dolomieu Lacepède (both Centrarchiformes: Centrarchidae), from the Duck River, Tennessee (USA). We emend Cryptogonimus to include features observed in the present specimens of its type species and in the descriptions of its congeners: a broad (wider than long) oral sucker, an intestine that bifurcates in the posterior half of the forebody, a bipartite seminal vesicle, a hermaphroditic duct that is dorsal to the ventral sucker, a preovarian seminal receptacle, and a Laurer's canal that opens dorsally at the level of the anterior testis. We describe Caecincola duttonae sp. n. (Cryptogonimidae) infecting largemouth bass, Micropterus salmoides (Lacepède), from Neely Henry Reservoir (Coosa River, Alabama, USA). The new species differs from its congeners by having a combination of a less elongate body, an intestine that bifurcates at the level of the ventral sucker, caeca that terminate at the level of the testes, diagonal testes in the middle of the hindbody, and a vitellarium predominantly distributed in the hindbody. We emend Caecincola Marshall et Gilbert, 1905 (type species Caecincola parvulus Marshall et Gilbert, 1905) to include features of the new species and recently-described congeners: an elongate body, an intestine that bifurcates in the posterior half of the forebody, caeca that extend posteriad beyond the testes, tandem testes, and a vitellarium that is wholly or primarily in the hindbody. Our 28S and ITS2 phylogenetic analyses recovered Caecincola and Cryptogonimus as sister taxa; Caecincola was recovered as paraphyletic with 28S but monophyletic with ITS2. This is the first phylogenetic study of Cryptogonimidae that includes a nucleotide sequence for a species of the type genus Cryptogonimus. We regard Cryptogonimus diaphanus (Stafford, 1904) Miller, 1941 as a species inquirenda.

Aquatic Parasitology Laboratory Southeastern Cooperative Fish Parasite and Disease Laboratory School of Fisheries Aquaculture and Aquatic Sciences College of Agriculture Auburn University Auburn Alabama USA

Tennessee Wildlife Resources Agency Nashville Tennessee USA

Unit for Environmental Sciences and Management North West University Potchefstroom South Africa *Address for correspondence Triet N Truong Aquatic Parasitology Laboratory Southeastern Cooperative Fish Parasite and Disease Laboratory School of Fisheries Aquaculture and Aquatic Sciences Auburn University 559 Devall Drive Auburn Alabama 36832 USA E mail ORCID 0000 0002 5556 2938

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Abell R.A., Olson D.M., Dinerstein E., Hurley P.T., Diggs J.T., Eichbaum W., Walters S., Wettengel W., Allnutt T., Loucks C.J., Hedao P. 2000: Freshwater Ecoregions of North America: A Conservation Assessment. Island Press, Washington, 319 pp.

Aguirre-Macedo M.L., Vidal-Martínez V.M., González-Solís D., Caballero P.I. 2007: Helminth communities of four commercially important fish species from Chetumal Bay, Mexico. J. Helminthol. 81: 19-31. PubMed DOI

Ahlstedt S.A., Powell J.R., Butler R.S., Fagg M.T., Hubbs D.W., Novak S.F., Palmer S.R., Johnson P.D. 2017: Historical and current examination of freshwater mussels (Bivalvia: Margaritiferidae: Unionidae) in the Duck River Basin Tennessee, USA Malacol. Rev. 45: 1-163.

Amin O.M. 1982: Adult trematodes (Digenea) from lake fishes of southeastern Wisconsin, with a key to species of the genus Crepidostomum Braun, 1900 in North America. Proc. Helminthol. Soc. Wash. 49: 196-206.

Anderson G.R., Barker S.C. 1998: Inference of phylogeny and taxonomy within the Didymozoidae (Digenea) from the second internal transcribed spacer (ITS2) of ribosomal DNA. Syst. Parasitol. 41: 87-94. DOI

Barger M.A. 2010: A new species of Caecincola (Trematoda: Cryptogonimidae) from spotted bass (Micropterus punctulatus) in the Big Thicket National Preserve, Texas, USA Comp. Parasitol. 77: 6-8. DOI

Barger M.A. 2018: Description of a new species of Caecincola (Trematoda: Cryptogonimidae) with a key to the species. Comp. Parasitol. 85: 66-72. DOI

Benz G.W., Collins D.E. (Eds.) 1997: Aquatic Fauna in Peril: The Southeastern Perspective. Lenz Design & Communications, Decatur, 554 pp.

Boschung H.T. 1961: An annotated list of fishes from the Coosa River system of Alabama. Am. Midl. Nat. 66: 257-285. DOI

Boschung H.T., Mayden R.L. 2004: Fishes of Alabama. Smithsonian Books, Washington D.C., USA, 960 pp.

Bray R.A., Waeschenbach A., Cribb T.H., Weedall G.D., Dyal P., Littlewood D.T.J. 2009: The phylogeny of the Lepocreadioidea (Platyhelminthes, Digenea) inferred from nuclearand mitochondrial genes: implications for their systematics and evolution. Acta Parasitol. 54: 310-329. DOI

Chernomor O., von Haeseler A., Minh B.Q. 2016: Terrace aware data structures for phylogenomic inference from supermatrices. Syst. Biol. 65: 997-1008. PubMed DOI

Chitwood F. 2016: Who owns Alabama's Coosa River? Citizens' impact on the tri-state water wars muted by private ownership of riparian rights. Virgin. Environ. Law J. 34: 230-254.

Corkum K.C. 1959: Some trematode parasites of fishes from the Mississippi Gulf Coast. Proc. Louisiana Acad. Sci. 22: 17-29.

Cribb T.H., Anderson G.R., Adlard R.D., Bray R.A. 1998: A DNA-based demonstration of a three-host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). Int. J. Parasitol. 28: 1791-1795. PubMed DOI

Curran S.S., Overstreet R.M. 2009: Caecincola longiscens n. sp. (Digenea: Cryptogonomidae) from the white crappie, Pomoxis annularis, in Mississippi, USA Comp. Parasitol. 76: 19-23. DOI

Duncan R.S. 2013: Southern Wonder: Alabama's Surprising Biodiversity. University of Alabama Press, Tuscaloosa, USA, 464 pp.

Fricke R., Eschmeyer W.N., Van der Laan R. (Eds.). 2024: Eschmeyer's catalog of fishes: genera, species, references. World Wide Web electronic publication, https://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp, 2/2024.

Gibson D.I. 1996: Part IV. Trematoda. In: L. Margolis and Z. Kabata (Eds.), Guide to the parasites of fishes of Canada. Can. Spec. Publ. Fish. Aquat. Sci. 124. NRC Research Press, Ottawa, Canada, pp. 1-373.

Greer G.J., Corkum K.C. 1979: Life cycle studies of three digenetic trematodes, including descriptions of two new species (Digenea: Cryptogonimidae). Proc. Helminthol. Soc. Wash. 46: 188-200.

Hernández-Orts J.S., Georgieva S., Landete D.N., Scholz T. 2019: Heterophyid trematodes (Digenea) from penguins: a new species of Ascocotyle Looss, 1899, first description of metacercaria of Ascocotyle (A.) patagoniensis Hernández-Orts, Montero, Crespo, Garcîa, Raga and Aznar, 2012, and first molecular data. Int. J. Parasitol. Parasites Wildl. 8: 94-105. PubMed DOI

Hoang D.T., Chernomor O., von Haeseler A., Minh B.Q., Vinh L.S. 2018: UFBoot2: Improving the ultrafast bootstrap approximation. Mol. Biol. Evol. 35: 518-522. PubMed DOI

ICZN 1999: International Code of Zoological Nomenclature. Fourth edition. International Trust for Zoological Nomenclature, London, 306 pp.

Jayawardena U.A., Tkach V.V., Navaratne A.N., Amerasinghe P.H., Rajakaruna R.S. 2013: Malformations and mortality in the Asian common toad induced by exposure to pleurolophocercous cercariae (Trematoda: Cryptogonimidae). Parasitol. Int. 62: 246-252. PubMed DOI

Kalyaanamoorthy S., Minh B.Q., Wong T.K.F., von Haeseler A., Jermiin L.S. 2017: ModelFinder: Fast model selection for accurate phylogenetic estimates. Nat. Methods 14: 587-589. PubMed DOI

Katoh K., Standley D.M. 2013: MAFFT Multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30: 772-780. PubMed DOI

Katokhin A.V., Shekhovtsov S.V., Konkow S., Yurlova N.I., Serbina E.A., Vodianitskaia S.N., Fedorov K.P., Loktev V.B., Muratov I.V., Ohyama F., Makhneva T.V., Pel'tek S.E., Mordvinov V.A. 2008: Assessment of the genetic distinctions of Opisthorchis felineus from O. viverrini and Clonorchis sinensis by ITS2 and CO1 sequences. Biochem. Biophys. Mol. Biol. 421: 214-217. PubMed DOI

Kmentová N., Bray R.A., Koblmüller S., Artois T., de Keyzer E.L.R., Gelnar M., Vanhove M.P.M., Georgieva S. 2020: Uncharted digenean diversity in Lake Tanganyika: cryptogonimids (Digenea: Cryptogonimidae) infecting endemic lates perches (Actinopterygii: Latidae). Parasit. Vectors 13: 221. PubMed DOI

Kvach Y., Bryjová A., Sasal P., Winkler H.M. 2018: The taxonomic and phylogenetic status of digeneans from the genus Timoniella (Digenea: Cryptogonimidae) in the Black and Baltic seas. J. Helminthol. 92: 596-603. DOI

Lockyer A.E., Olson P.D., Littlewood D.T.J. 2003: Utility of complete large and small subunit rRNA genes in resolving the phylogeny of the Neodermata (Platyhelminthes): implications and a review of the cercomer theory. Biol. J. Linn. Soc. 78: 155-171. DOI

Lundahl W.S. 1941: Life history of Caecincola parvulus Marshall and Gilbert (Cryptogonimidae, Trematoda) and the development of its excretory system. Trans. Am. Microsc. Soc. 60: 461-484. DOI

Marshall W.S., Gilbert N.C. 1905: Three new trematodes found principally in black bass. Zool. Jahrb. Abt. Syst. 22: 477-488.

Martin S.B., Cutmore S.C. 2022: Siphoderina hustoni n. sp. (Platyhelminthes: Trematoda: Cryptogonimidae) from the Maori snapper Lutjanus rivulatus (Cuvier) on the Great Barrier Reef. Syst. Parasitol. 99: 403-417. PubMed DOI

McAndrews K.L., Barger M.A. 2017: A new species of Caecincola (Trematoda: Cryptogonimidae) from white crappie (Pomoxis annularis) in southeastern Texas, USA Comp. Parasitol. 84: 32-35. DOI

Mettee M.F., O'Neil P.E., Pierson J.M. 1996: Fishes of Alabama and the Mobile Basin. Oxmoor House, Birmingham, Alabama, 820 pp.

Miller M.J. 1941: A critical study of Stafford's report on "trematodes of Canadian fishes" based on his trematode collection. Can. J. Res. 19D: 28-52. DOI

Miller T.L., Adlard R.D. 2020: Stemmatostoma cribbi n. sp. (Digenea: Cryptogonimidae) from freshwater fishes in the wet tropics bioregion of Queensland, Australia. J. Parasitol. 106: 411-417. PubMed DOI

Miller T.L., Adlard R.D., Bray R.A., Justine J.-L., Cribb T.H. 2010a: Cryptic species of Euryakaina n. g. (Digenea: Cryptogonimidae) from sympatric lutjanids in the Indo-West Pacific. Syst. Parasitol. 77: 185-204. PubMed DOI

Miller T.L., Bray R.A., Justine J.-L., Cribb T.H. 2010b: Varialvus gen. nov. (Digenea, Cryptogonimidae), from species of Lutjanidae (Perciformes) off the Great Barrier Reef, New Caledonia and the Maldives. Acta Parasitol. 55: 327-339. DOI

Miller T.L., Cribb T.H. 2007a: Two new cryptogonimid genera (Digenea, Cryptogonimidae) from Lutjanus bohar (Perciformes, Lutjanidae): analyses of ribosomal DNA reveals wide geographic distribution and presence of cryptic species. Acta Parasitol. 52: 104-113. DOI

Miller T.L., Cribb T.H. 2007b: Coevolution of Retrovarium n. gen. (Digenea: Cryptogonimidae) in Lutjanidae and Haemulidae (Perciformes) in the Indo-West Pacific. Int. J. Parasitol. 37: 1023-1045. PubMed DOI

Miller T.L., Cribb T.H. 2007c: Two new cryptogonimid genera Beluesca n. gen. and Chelediadema n. gen. (Digenea: Cryptogonimidae) from tropical Indo-West Pacific Haemulidae (Perciformes). Zootaxa 1543: 45-60. DOI

Miller T.L., Cribb T.H. 2008a: Eight new species of Siphoderina Manter, 1934 (Digenea, Cryptogonimidae) infecting Lutjanidae and Haemulidae (Perciformes) off Australia. Acta Parasitol. 53: 344-364. DOI

Miller T.L., Cribb T.H. 2008b: Family Cryptogonimidae Ward, 1917. In: R.A. Bray, D.I. Gibson and A. Jones (Eds.), Keys to the Trematoda. Volume 3. CAB International and Natural History Museum, Cambridge, pp. 51-112. DOI

Miller T.L., Cribb T.H. 2009: Gynichthys diakidnus n. g., n. sp. (Digenea: Cryptogonimidae) from the grunt Plectorhinchus gibbosus (Lacépède, 1802) (Perciformes: Haemulidae) off the Great Barrier Reef, Australia. Syst. Parasitol. 74:103-112. PubMed DOI

Miller T.L., Cribb T.H. 2013: Dramatic phenotypic plasticity within species of Siphomutabilus n. g. (Digenea: Cryptogonimidae) from Indo-Pacific caesionines (Perciformes: Lutjanidae). Syst. Parasitol. 86: 101-112. PubMed DOI

Miller T.L., Cutmore S.C., Cribb T.H. 2018: Two species of Neometadena Hafeezullah & Siddiqi, 1970 (Digenea: Cryptogonimidae) from Moreton Bay, Australia, including the description of Neometadena paucispina n. sp. from Australian Lutjanidae. Syst. Parasitol. 95: 655-664. PubMed DOI

Miller T.L., Downie A.J., Cribb T.H. 2009: Morphological disparity despite genetic similarity; new species of Lobosorchis Miller & Cribb, 2005 (Digenea: Cryptogonimidae) from the Great Barrier Reef and the Maldives. Zootaxa 1992: 37-52. DOI

Mueller J.F. 1934: Parasites of Oneida Lake fishes. Part IV. Additional notes on parasites of Oneida Lake fishes, including descriptions of new species. Roosevelt Wild Life Ann. 3: 335-373. DOI

Nahhas F.M., Short R.B. 1965: Digenetic trematodes of marine fishes from Apalachee Bay, Gulf of Mexico. Tulane Stud. Zool. 12: 39-50. DOI

Nguyen L.T., Schmidt H.A., von Haeseler A., Minh B.Q. 2015: IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol. Biol. Evol. 32: 268-274. PubMed DOI

Notton D., Michel E., Dale-Skey N., Nikolaeva S., Tracey S. 2011: Best practice in the use of the scientific names of animals: support for editors of technical journals. Bull. Zool. Nomencl. 68: 313-322. DOI

Olson P.D., Cribb T.H., Tkach V.V., Bray R.A., Littlewood D.T.J. 2003: Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). Int. J. Parasitol. 33: 733-755. PubMed DOI

Orcutt G., Barger M.A. 2017: A new species of Caecincola (Trematoda: Cryptogonimidae) from largemouth bass (Micropterus salmoides) in the Big Thicket National Preserve, Texas, USA Comp. Parasitol. 84: 155-158. DOI

Osborn H.L. 1903: On Cryptogonimus (n. g.) chili (n. sp.), a fluke with two ventral suckers. Zool. Anz. 26: 315-318.

Osborn H.L. 1910: On the structure of Cryptogonimus (nov. gen.) chyli (n. sp.), an aberrant distome, from fishes of Michigan and New York. J. Exp. Zool. 9: 517-536. DOI

Overstreet R.M., Cook J.O., Heard R.W. 2009: Trematoda (Platyhelminthes) of the Gulf of Mexico. In: D.L. Felder and D.K. Camp (Eds.), Gulf of Mexico Origin, Waters, and Biota. Volume 1, Biodiversity. Texas A & M University Press, College Station, Texas, pp. 419-486.

Pantoja C.S., Hernández-Mena D.I., Pérez-Ponce de León G., Luque J.L. 2018: Phylogenetic position of Pseudosellacotyla lutzi (Freitas, 1941) (Digenea: Cryptogonimidae), a parasite of Hoplias malabaricus (Bloch) in South America, through 28S rDNA sequences, and new observations of the ultrastructure of their tegument. J. Parasitol. 104: 530-538. PubMed DOI

Premvati G. 1967: Multigonotylus micropteri gen. et sp. n. and Caecincola wakullata sp. n. (Digenea: Phylogenetic position of Pseudosellacotyla lutzi (Freitas, 1941) (Digenea: Cryptogonimidae), a parasite of Hoplias malabaricus (Bloch) in South America, through 28S rDNA sequences, and new observations of the ultrastructure of their tegument Cryptogonimidae) from freshwater bass, Micropterus salmoides. J. Parasitol. 53: 743-746. DOI

Rambaut A., Suchard M.A., Xie D., Drummond A.J. 2014: FigTree v1.4.4. World Wide Web electronic publication, http://tree.bio.ed.ac.uk/software/figtree, 3/2024.

Santacruz A., Barluenga M., Pérez‑Ponce de León G. 2022: The macroparasite fauna of cichlid fish from Nicaraguan lakes, a model system for understanding host-parasite diversification and speciation. Sci. Rep. 12: 3944. PubMed DOI

Schilling E.M., Williams J.D. 2002: Freshwater mussels (Bivalvia: Margaritiferidae and Unionidae) of the Lower Duck River in Middle Tennessee: a historic and recent review. Southeast. Nat. 1: 403-414. DOI

Scholz T., Aguirre-Macedo L., Salgado-Maldonado G., Vargas-Vázquez J., Vidal-Martínez V., Wolter J., Kuchta R., Körting W. 1999: Redescription of Pseudoacanthostomum panamense Caballero, Bravo-Hollis, and Grogott, 1953 (Digenea: Acanthostomidae), a parasite of siluriform fishes of the family Ariidae, with notes on its biology. J. Helminthol. Soc. Wash. 66: 146-154.

Solodovnik D.A., Tatonova Y.V., Urabe M., Besprozvannykh V.V., Nakao M., Inoue K. 2021: Three species of Exorchis Kobayashi, 1921 (Digenea: Cryptogonimidae) in the East-Asian region: morphological and molecular data. Parasitology 148: 1578-1587. PubMed DOI

Stafford J. 1904: Trematodes from Canadian fishes. Zool. Anz. 27: 481-495.

Tennessee Wildlife Resources Agency (TWRA). 2024: Duck River in Tennessee. https://www.tn.gov/twra/fishing/where-to-fish/middle-tennessee-r2/duck-river, 10/2024.

Thaenkham U., Nawa Y., Blair D., Pakdee W. 2011: Confirmation of the paraphyletic relationship between families Opisthorchiidae and Heterophyidae using small and large subunit ribosomal DNA sequences. Parasitol. Int. 60: 521-523. PubMed DOI

Tkach V.V., Littlewood D.T.J., Olson P.D., Kinsella J.M., Swiderski Z. 2003: Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Syst. Parasitol. 56: 1-15. PubMed DOI

Van Cleave H.J., Mueller J.F. 1934: Parasites of Oneida Lake fishes. Part III. A biological and ecological survey of worm parasites. Roosevelt Wild Life Ann. 3: 161-334.

Ward H.B. 1917: On the structure and classification of North American parasitic worms. J. Parasitol. 4: 1-11. DOI

Wongsawad C., Wongsawad P., Sukontason K., Maneepitaksanti W., Nantarat N. 2017: Molecular phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) originated from freshwater fish from Chiang Mai Province, Thailand. Korean J. Parasitol. 55: 31-37. PubMed DOI

Young B.M., Hall, J.C. 2009: Headwaters: A Journey on Alabama Rivers. University of Alabama Press, Tuscaloosa, 192 pp.

Yong R.Q-Y., Martin S.B., Smit N.J. 2023: A new species of Siphoderina Manter, 1934 (Digenea: Cryptogonimidae) infecting the dory snapper Lutjanus fulviflamma (Teleostei: Lutjanidae) from the east coast of South Africa. Syst. Parasitol. 100: 673-686. PubMed DOI