Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

Applied Analytical Chemistry University of Duisburg Essen Essen Germany

Aston Medical School College of Health and Life Sciences Aston University Aston Triangle Birmingham UK

BPG Bio Framingham MA USA

Cayman Chemical Company Ann Arbor MI USA

Center for Experimental Therapeutics and Reperfusion Injury Department of Anesthesiology Perioperative and Pain Medicine Hale Building for Transformative Medicine Brigham and Women's Hospital and Harvard Medical School Boston MA USA

Center for Novel Target and Therapeutic Intervention College of Pharmacy Chongqing Medical University Chongqing P R China

Center of Membrane Biochemistry and Lipid Research University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden Dresden Germany

Centers for Disease Control and Prevention Atlanta GA USA

Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec Département de médecine Faculté de médecine Université Laval Québec City Canada

Centre for Cardiovascular Science Queen's Medical Research Institute University of Edinburgh Edinburgh UK

Centre for Metabolomics and Bioanalysis Faculty of Pharmacy CEU San Pablo University Madrid Spain

College of Health and Life Sciences Hamad Bin Khalifa University Doha Qatar

Core Facility Mass Spectrometry ZMF Medical University of Graz Graz Austria

Department of Analytical Chemistry University of Valencia Burjassot Spain

Department of Analytical Chemistry University of Vienna Vienna Austria

Department of Biochemistry Institute of Chemistry University of São Paulo São Paulo Brazil

Department of Chemistry and Biochemistry Concordia University Montreal Canada

Department of Chemistry and Industrial Chemistry University of Pisa Pisa Italy

Department of Chemistry University of Aveiro Aveiro Portugal

Department of Chemistry University of Washington Seattle WA USA

Department of Entomology University of California Davis CA USA

Department of Food Science and Technology University of California Davis CA USA

Department of Kinesiology and Health Sciences University of Waterloo Waterloo Canada

Department of Pharmaceutical and Medicinal Chemistry Institute of Pharmacy University of Greifswald Greifswald Germany

Department of Pharmaceutical Medicinal Chemistry Institute of Pharmacy Friedrich Schiller University Jena Germany

Department of Pharmacology and Chemical Biology University of Pittsburgh Pittsburgh PA USA

Department of Pharmacology and Department of Chemistry and Biochemistry University of California at San Diego La Jolla CA USA

Department of Pharmacology University of Colorado Denver Denver CO USA

Department of Respiratory Medicine and Allergy Karolinska University Hospital Stockholm Sweden

Department of Systems Pharmacology and Translational Therapeutics University of Pennsylvania Philadelphia PA USA

Division of Clinical Pharmacology Department of Medicine Vanderbilt University Medical Centre Nashville TN USA

Division of Intramural Research National Institute of Environmental Health Sciences National Institutes of Health Research Triangle Park Durham NC USA

Division of Pharmacy and Optometry School of Health Sciences Faculty of Biology Medicine and Health University of Manchester Manchester UK

Division of Rheumatology Department of Medicine Solna Karolinska Institutet and Karolinska University Hospital Stockholm Sweden

Edward A Doisy Department of Biochemistry and Molecular Biology Saint Louis University St Louis MO USA

Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

Faculty of Medicine Hebrew University of Jerusalem Jerusalem Israel

Faculty of Medicine Institute of Clinical Pharmacology Goethe University Frankfurt Frankfurt am Main Germany

Fraunhofer Cluster of Excellence of Immune Mediate Diseases CIMD Frankfurt am Main Germany

Fraunhofer Institute for Translational Medicine and Pharmacology ITMP Frankfurt am Main Germany

Health Sciences Mass Spectrometry Core University of Pittsburgh Pittsburgh PA USA

Indigo BioAutomation Carmel IN USA

Institut des Biomolécules Max Mousseron Pôle Chimie Balard Recherche CNRS Université de Montpellier ENSCN Montpellier France

Institute for Global Food Security Queen's University Belfast Belfast Northern Ireland

Institute for Translational Medicine and Therapeutics Perelman School of Medicine University of Pennsylvania Philadelphia PA USA

Institute of Clinical Chemistry and Laboratory Medicine University Hospital Regensburg Regensburg Germany

Institute of Laboratory Medicine Clinical Chemistry and Molecular Diagnostics University of Leipzig Medical Center Leipzig Germany

Institute of Pharmaceutical Chemistry Goethe University Frankfurt Frankfurt am Main Germany

Leiden University Medical Center Leiden Netherlands

Lipidomics Consulting Ltd Esbo Finland

Lipidomix GmbH Berlin Germany

Long Loo Lin School of Medicine National University of Singapore

Mass Spectrometry Core Facility Department of Chemistry Columbia University New York NY USA

Medical Research Council Mitochondrial Biology Unit and Department of Medicine University of Cambridge Cambridge UK

Medical School Royal Perth Hospital Unit University of Western Australia Perth Australia

MetaboHUB MetaToul I2MC Université de Toulouse Inserm Université Toulouse 3 Paul Sabatier Toulouse France

Metabolomics and Lipidomics Platform Faculty of Biology and Medicine University of Lausanne Lausanne Switzerland

Neonatal Research Group Health Research Institute La Fe Valencia Spain

Nestlé Institute of Food Safety and Analytical Sciences Société des Produits Nestlé Lausanne Switzerland

Nutrigenomics Research Group UCD Conway Institute School of Public Health Physiotherapy and Sports Science University College Dublin Ireland

RIKEN Center for Integrative Medical Sciences Yokohama Kanagawa Japan

Rowett Institute School of Medicine Medical Science and Nutrition University of Aberdeen Aberdeen UK

School of Biological Sciences Victoria University of Wellington Wellington New Zealand

School of Biosciences and Aston Institute for Membrane Excellence Aston University Aston Triangle Birmingham UK

School of Chemistry and Biochemistry Georgia Institute of Technology Atlanta GA USA

School of Mathematics and Natural Sciences University of Wuppertal Wuppertal Germany

School of Medicine Cardiff University Heath Park Cardiff UK

Shimadzu UK Ltd Milton Keynes UK

Signature Research Program in Cardiovascular and Metabolic Disorders Duke National University of Singapore Medical School Singapore Singapore

Singapore Lipidomics Incubator Life Sciences Institute and Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore

Unit of Integrative Metabolomics Institute of Environmental Medicine Karolinska Institutet Stockholm Sweden

Université Clermont Auvergne INRAE UNH Unité de Nutrition Humaine CRNH Auvergne Clermont Ferrand France

USDA ARS Western Human Nutrition Research Center and Department of Nutrition University of California Davis CA USA

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