The ongoing invasion of the zoonotic parasite Angiostrongylus cantonensis threatens humans, other mammals, and birds. In Mallorca, neurological disorders associated with this parasite are increasingly reported in free-living Algerian hedgehogs, Atelerix algirus, raising public health concerns. Experimental infections in the related species Atelerix albiventris were conducted to investigate the role of hedgehogs in the life cycle of the parasite. Captive-born hedgehogs were orally inoculated with 200 and 2000 third-stage larvae and monitored for 50 days by behavioral observations, blood tests (hematology and biochemistry), histopathology, and qPCR analysis. Baermann's larvoscopy was used to examine feces starting from day 39 post-infection (DPI). Neuropathological symptoms were only observed in the high-dose group, with larvae detected in brain tissue at 5, 15, 23, and 44 DPI. In contrast, no clinical symptoms or larvae in tissues were found in the low-dose group. Parasite DNA was present in the organs of both groups, but neurological migration occurred exclusively in the high-dose group. Blood tests showed no specific patterns indicating infection. Hedgehogs are aberrant hosts of A. cantonensis, showing severe neuropathological symptoms at high infection levels (2000 third-stage larvae), while lower doses remain asymptomatic. The parasite cannot complete its life cycle in A. albiventris hedgehogs, which do not contribute to its ecological circulation. However, hedgehogs can serve as sentinels for the spread of A. cantonensis in newly invaded regions like Mallorca. Monitoring neurological disease in wildlife could provide valuable insights into the potential spread of this zoonotic parasite in temperate latitudes.

Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic

Department of Comparative Pathobiology College of Veterinary Medicine Purdue University West Lafayette Indiana USA

Department of Veterinary Sciences Faculty of Agrobiology Food and Natural Resources CINeZ Czech University of Life Sciences Prague Praha Czech Republic

Institute of Parasitology Biology Center of Czech Academy of Sciences Ceske Budejovice Czech Republic

University of Veterinary Sciences Brno Brno Czech Republic

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Alicata, J. E. 1991. “The Discovery of PubMed

Anettová, L. , Izquierdo‐Rodriguez E., Foronda P., Baláž V., Novotný L., and Modrý D.. 2022. “Endemic Lizard PubMed PMC

Arango‐Colonna, M. , Delgado‐Serra S., Haines L. R., and Paredes‐Esquivel C.. 2023. “Improving the Detection of PubMed

Ash, L. R. 1968. “The Occurrence of PubMed

Barrett, J. , Carlisle M., and Prociv P.. 2002. “Neuro‐Angiostrongylosis in Wild Black and Grey‐Headed Flying Foxes ( PubMed

Bhaibulaya, M. 1975. “Comparative Studies on the Life History of PubMed

Burns, R. E. , Bicknese E. J., Qvarnstrom Y., et al. 2014. “Cerebral PubMed

Carlisle, M. , Prociv P., Grennan J., Pass M., Campbell G., and Mudie A.. 1998. “Cerebrospinal Angiostrongyliasis in Five Captive Tamarins ( PubMed

Červená, B. , Modrý D., Fecková B., et al. 2019. “Low Diversity of PubMed PMC

Chen, H. T. 1935. “Un Nouveau Nematode Pulmonaire,

Chotmongkol, V. , and Sawanyawisuth K.. 2002. “Clinical Manifestations and Outcome of Patients with Severe Eosinophilic Meningoencephalitis Presumably Caused by PubMed

Costa, L. R. R. , Mcclure J. J., Snider T. G., and Stewart T. B.. 2000. “Verminous Meningoencephalomyelitis by

Cowie, R. H. 2013. “Biology, Systematics, Life Cycle, and Distribution of PubMed PMC

Cowie, R. H. 2017. “ PubMed

Cowie, R. H. , Ansdell V., Dunavan C. P., and Rollins R. L.. 2022. “Neuroangiostrongyliasis: Global Spread of an Emerging Tropical Disease.” American Journal of Tropical Medicine and Hygiene 107: 1166–1172. PubMed PMC

Cowie, R. H. , Malik R., and Morgan E. R.. 2023. “Comparative Biology of Parasitic Nematodes in the Genus PubMed

Dalton, M. F. , Fenton H., Cleveland C. A., Elsmo E. J., and Yabsley M. J.. 2017. “Eosinophilic Meningoencephalitis Associated with Rat Lungworm ( PubMed PMC

Delgado‐Serra, S. , Sola J., Negre N., and Paredes‐Esquivel C.. 2022. “ PubMed PMC

Diao, Z. , Wang J., Qi H., Li X., Zheng X., and Yin C.. 2011. “Human Ocular Angiostrongyliasis: A Literature Review.” Tropical Doctor 41: 76–78. PubMed

Dobey, C. L. , Grunenwald C., Newman S. J., Muller L., and Gerhold R. W.. 2014. “Retrospective Study of Central Nervous System Lesions and Association with PubMed

Duffy, M. S. , Miller C. L., Kinsella J. M., and de Lahunta A.. 2004. “ PubMed PMC

Dunn, A. M. 2009. “Parasites and Biological Invasions.” Advances in Parasitology 68: 161–184. PubMed

Edwards, E. E. , Borst M. M., Lewis B. C., Gomez G., and Flanagan J. P.. 2020. “ PubMed

Emerson, J. A. , Walden H. S., Peters R. K., et al. 2013. “Eosinophilic Meningoencephalomyelitis in an Orangutan ( PubMed

Fuentes, M. V. , Gomez‐Samblas M., Richter O., et al. 2024. “Local Terrestrial Snails as Natural Intermediate Hosts of the Zoonotic Parasite PubMed

Gardiner, C. H. , Wells S., Gutter A. E., et al. 1990. “Eosinophilic Meningoencephalitis Due to PubMed

Gosnell, W. L. , and Kramer K. J.. 2013. “The Role of Eosinophils in Angiostrongyliasis: Multiple Roles for a Versatile Cell?” Hawai'i Journal of Medicine & Public Health: a Journal of Asia Pacific Medicine & Public Health 72, no. Supplement 2: 49–51. PubMed PMC

Ishida, K. , and Yoshimura K.. 1990. “Differences in Responses of Rat‐ and Guinea‐Pig‐Eosinophils to Eosinophil Chemotactic Factors Derived from PubMed

Jarvi, S. , Jacob J., Mina A., and Lyons M.. 2024. “Detection of Rat Lungworm ( PubMed PMC

Jarvi, S. , and Prociv P.. 2021. “ PubMed PMC

Jarvi, S. I. , Pitt W. C., Farias M. E., et al. 2015. “Detection of PubMed PMC

Jaume‐Ramis, S. , Martínez‐Ortí A., and Delgado‐Serra S., et al. 2023. “Potential Intermediate Hosts of PubMed PMC

Ji, L. , Yiyue X., Xujin H., et al. 2017. “Study on the Tolerance and Adaptation of Rats to PubMed

Kim, D. Y. , Stewart T. B., Bauer R. W., and Mitchell M.. 2002. “ PubMed

Kodama, H. , Koyama T., Takasaka M., et al. 1981. “Jikken dobutsu.” Experimental Animals 30: 251–261. PubMed

Kottwitz, J. J. , Perry K. K., Rose H. H., and Hendrix C. M.. 2014. “ PubMed

Kwong, Y. W. , and Dobson C.. 1982. “Population Dynamics of PubMed

Lan, K. P. , Wang C. J., Hsu J. D., Chen K. M., Lai S. C., and Lee H. H.. 2004. “Induced Eosinophilia and Proliferation in PubMed

Lodge, D. M. , Taylor C. A., Holdich D. M., and Skurdal J.. 2000. “Nonindigenous Crayfishes Threaten North American Freshwater Biodiversity: Lessons from Europe.” Fisheries 25: 7–20.

Lunn, J. A. , Lee R., Smaller J., et al. 2012. “Twenty Two Cases of Canine Neural Angiostrongylosis in Eastern Australia (2002‐2005) and a Review of the Literature.” Parasites and Vectors 5: 70. PubMed PMC

Lv, S. , Zhang Y., Steinmann P., et al. 2011. “The Emergence of Angiostrongyliasis in the People's Republic of China: The Interplay Between Invasive Snails, Climate Change and Transmission Dynamics.” Freshwater Biology 56: 717–734.

Martins, Y. C. , Tanowitz H. B., and Kazacos K. R.. 2015. “Central Nervous System Manifestations of PubMed PMC

McKenzie, R. A. , Green P. E., and Wood A. D.. 1978. “ PubMed

Modrý, D. , Fecková B., Putnová B., Manalo S. M., and Otranto D.. 2021. “Alternative Pathways in PubMed PMC

Pan, C. , Liang S., Ling H., et al. 2000. “Experimental Observation on the Distribution of

Paredes‐Esquivel, C. , Sola J., Delgado‐Serra S., et al. 2019. “ PubMed PMC

Patial, S. , Delcambre B. A., DiGeronimo P. M., Conboy G., Vatta A. F., and Bauer R.. 2022. “Verminous Meningoencephalomyelitis in a Red Kangaroo Associated with PubMed PMC

Peng, S. Y. , Lam H. Y. P., and Huang Y. T.. 2024. “Defective Glycolysis in the Cerebrum and Cerebellum Correlates with the Pathology and Neurological Declines in Mice with PubMed

Perez, O. , Capron M., Lastre M., Venge P., Khalife J., and Capron A.. 1989. “ PubMed

Pirisi, M. , Gutiérrez Y., Minini C., et al. 1995. “Fatal Human Pulmonary Infection Caused by an Angiostrongylus‐Like Nematode.” Clinical Infectious Diseases 20: 59–65. PubMed

Prenter, J. , MacNeil C., Dick J. T. A., and Dunn A. M.. 2004. “Roles of Parasites in Animal Invasions.” Trends in Ecology and Evolution 19: 385–390. PubMed

Prociv, P. , and Carlisle M. S.. 2001. “The Spread of PubMed

Prociv, P. , and Turner M.. 2018. “Neuroangiostrongyliasis: The “Subarachnoid Phase” and Its Implications for Anthelminthic Therapy.” American Journal of Tropical Medicine and Hygiene 98: 353–359. PubMed PMC

Rael, R. C. , Peterson A. C., Ghersi‐Chavez B., Riegel C., Lesen A. E., and Blum M. J.. 2018. “Rat Lungworm Infection in Rodents Across Post‐Katrina New Orleans, Louisiana, USA.” Emerging Infectious Diseases 24: 2176–2183. PubMed PMC

Rizor, J. , Yanez R. A., Thaiwong T., and Kiupel M.. 2022. “ PubMed PMC

Rosen, L. , Chappell R., Laqueur G. L., Wallace G. D., and Weinstein P. P.. 1962. “Eosinophilic Meningoencephalitis Caused by a Metastrongylid Lung‐Worm of Rats.” The Journal of the American Medical Association 179: 620–624. PubMed

Sears, W. J. , Qvarnstrom Y., Dahlstrom E., et al. 2021. “AcanR3990 qPCR: A Novel, Highly Sensitive, Bioinformatically‐Informed Assay to Detect PubMed PMC

Shamsi, S. , Miles S., Tozer K., Raidal S. R., and Smales L. R.. 2019. “The First Report of Cerebral, Pulmonary and Gastrointestinal Angiostrongyliasis in the Water Rat PubMed

Spratt, D. M. 2015. “Species of PubMed PMC

Summers, B. A. , Cummings J. F., and de Lahunta A.. 1995. “Inflammatory Disease of the Central Nervous System.” In Veterinary Neuropathology, edited by Duncan L., 159–170. Mosby.

Thiengo, S. C. , Maldonado A., Mota E. M., et al. 2010. “The Giant African Snail PubMed

Tuan, Y. C. , Kuo H. Y., Yin J. H., et al. 2017. “Case Report: Angiostrongyliasis in a Pallas's Squirrel (

Turck, H. C. , Fox M. T., and Cowie R. H.. 2022. “Paratenic Hosts of PubMed PMC

Walden, H. D. S. , Slapcinsky J., Rosenberg J., and Wellehan J. F. X.. 2021. “ PubMed PMC

Wallace, G. D. , and Rosen L.. 1969. “Studies on Eosinophilic Meningitis.VI. Experimental Infection of Rats and Other Homoiothermic Vertebrates with PubMed

Wang, L. C. , Jung S. M., Chen C. C., Wong H. F., Wan D. P., and Wan Y. L.. 2006. “Pathological Changes in the Brains of Rabbits Experimentally Infected with PubMed

Wang, L. C. , Jung S. M., Chen K. Y., Wang T. Y., and Li C. H.. 2015. “Temporal‐Spatial Pathological Changes in the Brains of Permissive and Non‐Permissive Hosts Experimentally Infected with PubMed

Wang, Q. P. , Lai D. H., Zhu X. Q., Chen X. G., and Lun Z. R.. 2008. “Human Angiostrongyliasis.” The Lancet Infectious Diseases 8: 621–630. PubMed

Wright, J. D. , Kelly W. R., Wadell A. H., and Hamilton J.. 1991. “Equine Neural Angiostrongylosis.” Australian Veterinary Journal 68: 58–60. PubMed

Same parasite, diverging fates: distinct responses of tenrecs to Angiostrongylus cantonensis infection