Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in a high-contiguity chromosome-level assembly. We implemented a comprehensive annotation pipeline incorporating gene models and functional annotations from the Solanum tuberosum Phureja DM reference genome alongside RNA-seq reads to provide high-quality gene and transcript annotations. Additionally, we provide a genome-wide DNA methylation profile using Oxford Nanopore reads, enabling insights into potato epigenetics. The assembled genome, annotations, methylation and expression data are visualised in a publicly accessible genome browser, providing a valuable resource for the potato research community.

Faculty of Mathematics and Natural Sciences Institute for Biological Data Science Cluster of Excellence on Plant Sciences Heinrich Heine University Düsseldorf Düsseldorf Germany

Institute of Bio and Geosciences CEPLAS Forschungszentrum Jülich GmbH Jülich Germany

Jožef Stefan International Postgraduate School Ljubljana Slovenia

Molecular Systems Biology Department of Functional and Evolutionary Ecology University Vienna Vienna Austria

National Institute of Biology Department of Biotechnology and Systems Biology Ljubljana Slovenia

Plant Stress Resilience Institute of Environmental Biology Utrecht University Utrecht The Netherlands

PSI Drásov Czech Republic

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