Elucidation of new sulfamethoxazole catabolic pathways in whole-cell catalyst of bacterium Kocuria rhizophila SA117
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
40582424
DOI
10.1016/j.biortech.2025.132912
PII: S0960-8524(25)00878-8
Knihovny.cz E-resources
- Keywords
- Biodegradation, Genome sequencing, Metabolic profiling, Proteomics, Sulfonamide,
- MeSH
- Biodegradation, Environmental MeSH
- Phylogeny MeSH
- Catalysis MeSH
- Metabolic Networks and Pathways * MeSH
- Micrococcaceae * metabolism genetics MeSH
- Proteomics MeSH
- Sulfamethoxazole * metabolism MeSH
- Tandem Mass Spectrometry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Sulfamethoxazole * MeSH
Sulfamethoxazole (SMX) and its residues exhibit high environmental persistence due to their resistance to conventional degradation processes. The bacterial strain Kocuria rhizophila SA117, isolated from polluted soils, was characterized biochemically, phylogenetically, and -omically. Herein, we describe a complete degradation pathway for SMX and determine two putative pathways: cleavage of the benzene ring and the degradation of the substituted isoxazole, leading to the formation of non-toxic Krebs cycle metabolites. Based on molecular structures containing 13C6-labeled carbons and 2H3 atoms, thirty metabolites were identified by high-resolution tandem mass spectrometry. Genomic and proteomic analysis of strain SA117 revealed its ability to perform a wide range of metabolic activities under sulfamethoxazole selective pressure. These activities include energy and sulfur metabolism, adaptation to stress conditions, and catabolism of aromatic compounds. This study has greatly enhanced the understanding of microbial sulfonamide degradation and highlighted the potential of the bacterium Kocuria in remediation strategies.
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