In order to proliferate, bacteria must remodel their cell wall at the division site. The division process is driven by the enzymatic activity of peptidoglycan synthases and hydrolases around the constricting Z-ring. We introduce a morphoelastic model that correctly reproduces the shape of the division site during the constriction and septation phases of Escherichia coli. In the model, mechanical stress directs the transformation of the bacterial wall. The two constants associated with growth and remodeling respectively are its only adjustable parameters. Different morphologies, corresponding either to mutant or wild type cells, are recovered as a function of the remodeling parameter. In addition, a plausible range for the cell stiffness and turgor pressure was determined by comparing numerical simulations with bacterial cell plasmolysis data.

Department of Fundamental Microbiology University of Lausanne Quartier UNIL Sorge Lausanne 1015 Switzerland

Department of Genetics Blavatník Institute Harvard Medical School Boston MA 02115

Department of Molecular Biology Massachusetts General Hospital Boston MA 02114

Mathematical Institute Faculty of Mathematics and Physics Charles University Praha 18675 Czech Republic

Před aktualizací

bioRxiv. 2024 Sep 12:2024.09.11.612282. doi: 10.1101/2024.09.11.612282. PubMed

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