Neodothiora populina is a black yeast-like fungus in the family of Dothioraceae. It causes an aggressive canker disease of trembling aspen that results in widespread mortality of aspen across the boreal forest of Interior Alaska. Here we report a high-quality draft genome including functional annotation of this emerging fungal pathogen based on Nanopore Technology long-read sequences. Our initial genome assembly totaled 23,960,169 bp and contained 18 contigs and we identified 7,343 genes. This resource announcement provides new genomic data useful long-term to improve our understanding of forest health in Alaska.

Center of Genomics and Bioinformatics Indiana University Bloomington IN 47405

Department of Biochemistry and Microbiology Faculty of Food and Biochemical Technology University of Chemistry and Technology Prague Technicka 3 166 28 Prague 6 Czech Republic

Department of Biology and Wildlife University of Alaska Fairbanks Fairbanks AK 99775

Department of Plant Pathology University of Nebraska Lincoln Lincoln NE 68583

Institute of Arctic Biology University of Alaska Fairbanks Fairbanks AK 99775

Southwestern University Georgetown TX

United States Department of Agriculture Forest Service Forest Health Protection Fairbanks Alaska 99709

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