This study integrates archaeobotanical and stable isotopic data to investigate dietary adaptations and crop use in past Central European societies during later prehistory (500-0 BCE), a period marked by growing social complexity and technological innovation that approximates sub-modern population structures. Using a multi-proxy approach, we analyse supra-regional datasets of plant macro-remains and human bone collagen from Bohemia, Moravia, and Slovakia, focusing on millet, the only C₄ crop cultivated in preindustrial temperate Europe, which serves as a distinct isotopic marker for tracing subsistence shifts. Archaeobotanical evidence, assessed through minimum number of individuals (MNI), ubiquity, and Representativeness Index, shows that millet cultivation was largely influenced by local environmental conditions, with no consistent temporal trend across regions. In contrast, stable carbon isotope data reveal a systemic dietary shift towards higher δ13C values during the third century BCE, coinciding with technological advances and increasing socio-economic complexity. This new trend, however, was not applied consistently: its intensity varied across sites and social groups, reflecting flexible and context-specific subsistence strategies. Non-elite individuals exhibited more pronounced shifts, while elite groups maintained more conservative, C₃-based diets. By demonstrating broad patterns alongside local variability, this study highlights the flexibility and adaptive capacity of past food production. The findings underscore the importance of integrating both regional and local scales in multi-proxy analyses and provide insights relevant to modern contexts facing globalised yet ecologically diverse agricultural challenges.

Constantine the Philosopher University Nitra Slovakia

Czech Academy of Sciences Institute of Archaeology Prague Prague Czech Republic

Czech Academy of Sciences Nuclear Physics Institute Prague Czech Republic

Moravian Museum Brno Czech Republic

University of South Bohemia in České Budějovice České Budějovice Czech Republic

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Militký, J., Kysela, J. & Tisucká, M. (National Museum Prague, 2019).

Danielisová, A., Bursák, D., Strnad, L., Trubač, J. & Fikrle, M. in 5th International Conference "Archaeometallurgy in Europe" 19–21 June 2019, Miskolc, Hungary Vol. 73 Monographies Instrumentum (eds Béla Török & A. Giumlia-Mair) 273–288 (Éditions Mergoil, 2021).

Venclová, N. Němčice and Staré Hradisko. Iron Age glass and glass-working in Central Europe., 317 (Institute of Archaeology 2016).

Venclová, N. & Militky, J. in Produktion - Distribution - Ökonomie. Siedlungs- und Wirtschaftsmuster der Latenezeit. Universitätsforschungen zur prähistorischen Archäologie Bd. 258 (ed S. Hornung) 387–406 (Habelt, 2014).

Sankot, P. in Moravské křižovatky. Střední Podunají mezi pravěkem a historií (ed J.; Venclová Čižmářová, N.; Březinová, G.) 255–271 (Moravské zemské muzeum, 2014).

Militky, J. Keltské mincovnictví ve 3. a 2. století před Kristem v Čechách ((Keltisches Münzwesen im 3. und 2. Jahrhundert vor Christus /LT B2 bis LT C/ in Böhmen). (Archaeological Institute of the CAS, 2018).

Dreslerová, D. & Kočár, P. Trends in cereal cultivation in the Czech Republic from the Neolithic to the Migration period (5500 b.c.–a.d. 580). Vegetation History and Archaeobotany 22, 257–268 (2013). 10.1007/s00334-012-0377-8

Hajnalová, E. Súčasné poznatky z archeobotaniky na Slovensku.

Hajnalová, E. (Archaeological Institute of the Slovak Academy of Sciences, Nitra, 1991).

Kohler-Schneider, M., Caneppele, A. & Heiss, A. G. Land use, economy and cult in late Iron Age ritual centres: an archaeobotanical study of the La Tène site at Sandberg-Roseldorf, Lower Austria. DOI

Gyulai, F. Archaeobotany in Hungary. Seed, Fruit, Food and Beverage Remains in the Carpathian Basin from the Neolithic to the Late Middle Ages., (Archaeolingua, 2010).

Saliari, K. & Trebsche, P. Cattle Make the Difference: Variations and Developments of Animal Husbandry in the Central European La Tène Culture. PubMed DOI PMC

Knipper, C. et al. What is on the menu in a Celtic town? Iron Age diet reconstructed at Basel-Gasfabrik, Switzerland. DOI

Heiss, A. G. & Kohler-Schneider, M. in Produktion — Distribution — Ökonomie. Siedlungs- und Wirtschaftsmuster der Latènezeit. Akten des internationalen Kolloquiums in Otzenhausen, 28.–30. Oktober 2011. (ed S. Hornung) 375–386 (Dr. Rudolf Habelt GmbH, 2014).

Dálnoki, O. & Jacomet, S. Some aspects of Late Iron Age agriculture based on the first results of an archaeobotanical investigation at Corvin tér, Budapest, Hungary. DOI

Motuzaitė Matuzevičiūtė, G. Broomcorn Millet: From the Past to the Future. DOI

Kirleis, W., Corso, M. D. & Filipović, D. Millet and What Else? The Wider Context of the Adoption of Millet Cultivation in Europe., (Sidestone Press, 2022).

Filipović, D. et al. New AMS 14C dates track the arrival and spread of broomcorn millet cultivation and agricultural change in prehistoric Europe. PubMed DOI PMC

Lightfoot, E., Liu, X. & Jones, M. K. Why move starchy cereals? A review of the isotopic evidence for prehistoric millet consumption across Eurasia. DOI

Cavazzuti, C. et al. Isotope and archaeobotanical analysis reveal radical changes in mobility, diet and inequalities around 1500 BCE at the core of Europe. PubMed DOI PMC

Smith, M. L. The archaeology of food preference. DOI

Kellner, C. M. & Schoeninger, M. J. A simple carbon isotope model for reconstructing prehistoric human diet. PubMed DOI

Ambrose, S. H. & Norr, L. in Prehistoric Human Bone: Archaeology at the Molecular Level (eds Joseph B. Lambert & Gisela Grupe) 1–37 (Springer, Berlin and Heidelberg, 1993).

Kohn, M. J. Carbon isotope compositions of terrestrial C3 plants as indicators of (paleo) ecology and (paleo) climate. PubMed DOI PMC

Still, C. J., Berry, J. A., Collatz, G. J. & Defries, R. S. Global distribution of C3 and C4 vegetation: Carbon cycle implications. DOI

Sage, R. F., Christin, P.-A. & Edwards, E. J. The C4 plant lineages of planet Earth. PubMed DOI

Eggels, S., Blankenagel, S., Schön, C. C. & Avramova, V. The carbon isotopic signature of C(4) crops and its applicability in breeding for climate resilience. PubMed DOI PMC

Taylor, S. H. et al. Physiological advantages of C4 grasses in the field: A comparative experiment demonstrating the importance of drought. PubMed DOI PMC

Osborne, C. P. & Sack, L. Evolution of C4 plants: a new hypothesis for an interaction of CO PubMed DOI PMC

Hunt, H. V. et al. Millets across Eurasia: chronology and context of early records of the genera Panicum and Setaria from archaeological sites in the Old World. PubMed DOI PMC

Hedges, R. E. M. On bone collagen—apatite-carbonate isotopic relationships. DOI

Bocherens, H. & Drucker, D. Trophic level isotopic enrichment of carbon and nitrogen in bone collagen: case studies from recent and ancient terrestrial ecosystems. DOI

Le Huray, J. D. Dietary Reconstruction and Social Stratification during the Iron Age in Central Europe. An Examination of Palaeodiet, Migration, and Diagenesis Using Stable Isotope and Trace Element Analysis of Archaeological Bone Samples from the Czech Republic. Ph.D. thesis, University of Bradford, (2006).

Lightfoot, E., Šlaus, M., Šikanjić, P. R. & O’Connell, T. C. Metals and millets: Bronze and Iron Age diet in inland and coastal Croatia seen through stable isotope analysis. DOI

Depaermentier, M. L. C. Isotope data in Migration Period archaeology: Critical review and future directions. DOI

Pospieszny, Ł et al. Isotopic evidence of millet consumption in the Middle Bronze Age of East-Central Europe. DOI

Laffranchi, Z., Huertas, A. D., Jiménez Brobeil, S. A., Torres, A. G. & Riquelme Cantal, J. A. Stable C & N isotopes in 2100 Year-BP human bone collagen indicate rare dietary dominance of C4 plants in NE-Italy. PubMed DOI PMC

Orfanou, E. et al. Biomolecular evidence for changing millet reliance in Late Bronze Age central Germany. PubMed DOI PMC

Valamoti, S. M. Millet, the late comer: On the tracks of Panicum miliaceum in prehistoric Greece. DOI

Wang, T. et al. Tianshanbeilu and the Isotopic Millet Road: reviewing the late Neolithic/Bronze Age radiation of human millet consumption from north China to Europe. PubMed DOI PMC

Gamarra, B. et al. 5000 years of dietary variations of prehistoric farmers in the Great Hungarian Plain. PubMed DOI PMC

Varalli, A. et al. Bronze Age innovations and impact on human diet: A multi-isotopic and multi-proxy study of western Switzerland. PubMed DOI PMC

Varalli, A., Moggi-Cecchi, J. & Goude, G. A multi-proxy bioarchaeological approach reveals new trends in Bronze Age diet in Italy. PubMed DOI PMC

Tafuri, M. A., Craig, O. E. & Canci, A. Stable isotope evidence for the consumption of millet and other plants in Bronze Age Italy. PubMed DOI

Hernando, R. et al. Integrating buccal and occlusal dental microwear with isotope analyses for a complete paleodietary reconstruction of Holocene populations from Hungary. PubMed DOI PMC

González-Rabanal, B., Marín-Arroyo, A. B., Cristiani, E., Zupancich, A. & González-Morales, M. R. The arrival of millets to the Atlantic coast of northern Iberia. PubMed DOI PMC

Motuzaite Matuzeviciute, G., Staff, R., Hunt, H. & Liu, X. The early chronology of broomcorn millet (Panicum Miliaceum) in Europe. DOI

Ventresca Miller, A. R. et al. Re-evaluating Scythian lifeways: Isotopic analysis of diet and mobility in Iron Age Ukraine. PubMed DOI PMC

Martin, L. et al. The place of millet in food globalization during Late Prehistory as evidenced by new bioarchaeological data from the Caucasus. PubMed DOI PMC

Salova, J., Vyazov, L. & Beneš, J. When Barley and Wheat Meet Millet: Cereal Cultivation Patterns in the Forest and Forest-Steppe of Eastern Europe from the Early Iron Age to the Early Middle Ages. DOI

Gyulai, F. The history of broomcorn millet (Panicum miliaceum L.) in the Carpathian-basin in the mirror of archaeobotanical remains II. From the Roman age until the late medieval age. DOI

Hajnalová, M. Archeobotanika doby bronzovej na Slovensku. Štúdie ku klíme, prírodnému prostrediu, poľnohospodárstvu a paleoekonómii. (Univerzita Konštantína Filozofa v Nitre, Filozofická fakulta, 2012).

Toulemonde, F. et al. in Millet and What Else? The Wider Context of the Adoption of Millet Cultivation in Europe Millet and what else? The wider context of the adoption of millet cultivation in Europe. 155–184 (Sidestone Press Academics, 2022).

Šálková, T. et al. Crops along the trade routes? Archaeobotany of the Bronze Age in the region of South Bohemia (Czech Republic) in context with longer distance trade and exchange networks. DOI

Moghaddam, N., Müller, F. & Lösch, S. A bioarchaeological approach to the Iron Age in Switzerland: Stable isotope analyses (δ13C, δ15N, δ34S) of human remains. DOI

Vytlačil, Z., Danielisová, A., Velemínský, P., Blažek, J. & Drtikolová Kaupová, S. Dietary changes seen through the isotope analysis of the La Tène burial site of Prosmyky (Bohemia, 4th-3rd century BCE). DOI

Abdrakhmanov, M. et al. The shifting of buffer crop repertoires in pre-industrial north-eastern Europe. PubMed DOI PMC

Reitsema, L. J. & Vercellotti, G. Stable isotope evidence for sex- and status-based variations in diet and life history at medieval Trino Vercellese Italy. PubMed DOI

Maxwell, A. B.

Laffranchi, Z. et al. Funerary reuse of a Roman amphitheatre: Palaeodietary and osteological study of Early Middle Ages burials (8th and 9th centuries AD) discovered in the Arena of Verona (Northeastern Italy). DOI

Iacumin, P., Galli, E., Cavalli, F. & Cecere, L. C4-consumers in southern europe: The case of friuli V.G. (NE-Italy) during early and central middle ages. PubMed DOI

Kaupová, S. et al. Diet in transitory society: isotopic analysis of medieval population of Central Europe (ninth–eleventh century AD, Czech Republic). DOI

Lightfoot, E., Šlaus, M. & O’Connell, T. C. Changing cultures, changing cuisines: Cultural transitions and dietary change in iron age, roman, and early medieval croatia. PubMed DOI

Knipper, C. et al. Dietary reconstruction in Migration Period Central Germany: a carbon and nitrogen isotope study. DOI

Plecerová, A., Kaupová Drtikolová, S., Šmerda, J., Stloukal, M. & Velemínský, P. Dietary reconstruction of the Moravian Lombard population (Kyjov, 5th–6th centuries AD, Czech Republic) through stable isotope analysis (δ13C, δ15N). DOI

Sebald, S. V., Grupe, G. & Hakenbeck, S. Isotopic sourcing reveals changing subsistence, cultural diversity and biological correlates with palaeodiet in fourth- to seventh-century AD Bavaria. DOI

Moghaddam, N., Müller, F., Hafner, A. & Lösch, S. Social stratigraphy in Late Iron Age Switzerland: stable carbon, nitrogen and sulphur isotope analysis of human remains from Münsingen. DOI

Danielisova, A. in Moving forward. Professor Volker Heyd’s Festschrift as he turns 60 Vol. 11 Monographs of the Archaeological Society of Finland (eds A. Lahelma et al.) 193–208 (Archaeological Society of Finland, 2023).

Zavodny, E., Culleton, B. J., McClure, S. B., Kennett, D. J. & Balen, J. Minimizing risk on the margins: Insights on Iron Age agriculture from stable isotope analyses in central Croatia. DOI

Kuneš, P. et al. The origin of grasslands in the temperate forest zone of east-central Europe: long-term legacy of climate and human impact. PubMed DOI PMC

Čižmář, I. & Danielisová, A. Central sites and the development of rural settlements from the middle to Late La Tène period in central Moravia. DOI

Mohammadi, S., Rydgren, K., Bakkestuen, V. & Gillespie, M. A. K. Impacts of recent climate change on crop yield can depend on local conditions in climatically diverse regions of Norway. PubMed DOI PMC

Čižmář, I. et al. Výsledky terénního výzkumu v roce 2021 a nejstarší sklářská dílna v zaalpské Evropě | Němčice. Results of Field Research in 2021 and the Oldest Glass Workshop in Transalpine Europe. DOI

Scheeres, M. et al. “Celtic migrations”: Fact or fiction? Strontium and oxygen isotope analysis of the Czech cemeteries of Radovesice and Kutná Hora in Bohemia. PubMed DOI

Danielisová, A. et al. Claiming the land or protecting the goods? The Duchcov hoard in Bohemia as a proxy for ‘Celtic migrations’ in Europe in the 4th century BCE. DOI

Depaermentier, M. L. C., Kempf, M., Bánffy, E. & Alt, K. W. Modelling a scale-based strontium isotope baseline for Hungary. DOI

Fňukalová, E. & Romportl, D. A typology of natural landscapes of Central Europe. DOI

Beech, M. in Mšecké Žehrovice in Bohemia: archaeological background to a Celtic hero, 3rd-2nd cent. B.C. (ed N. Venclová) 225 – 304 (Kronos, 1998).

Crabtree, P. in Settlement, Economy and Cultural Change at the End of the European Iron Age (ed Peter Wells) 61–65 (International Monographs in Prehistory, 1994).

Méniel, P. Le site protohistorique d´Acy-Romance (Ardennes) – III. Les animaux et l´histoire d´un village Gaulois. Fouilles 1987 – 1997., Vol. 14 (Société archéologique champenoise, 1998).

Peške, L. in Die Hallstatt- und Latènezeitliche Siedlung mit Gräberfeld bei Radovesice in Böhmen. I, II. Vol. 21 Archeologické výzkumy v severních Čechách (ed J. Waldhauser) 156–172 (ÚAPP 1993).

Danielisová, A., Olševičová, K., Cimler, R. & Machálek, T. in Agent-based Modeling and Simulation in Archaeology (eds Gabriel Wurzer, Kerstin Kowarik, & Hans Reschreiter) 183–216 (Springer International Publishing, 2015).

Danielisova, A. & Hajnalová, M. in Produktion- Distribution - Ökonomie. Siedlungs-und Wirtschaftsmuster der Latènezeit, Akten des internationalen Kolloquiums in Otzenhausen, 28.-30. Oktober 2011 Universitätsforschungen zurprähistorischen Archäologie (ed S. Hornung) 407–428 (Verlag Dr. Rudolf Habelt GmbH, 2014).

de Gruchy, Y. A. et al. Bone of contention: Intra-element variability in remodelling of human femora based on histomorphometric and isotope analyses. PubMed DOI PMC

Hedges, R. E. & Reynard, L. M. Nitrogen isotopes and the trophic level of humans in archaeology. DOI

Fahy, G. E., Deter, C., Pitfield, R., Miszkiewicz, J. J. & Mahoney, P. Bone deep: Variation in stable isotope ratios and histomorphometric measurements of bone remodelling within adult humans. DOI

Venclová, N. The Prehistory of Bohemia 6, The La Tene period., (Archaeological Institute in Prague, 2013).

ter Braak, C. F. J. & Smilauer, P. CANOCO Reference Manual and CanoDraw for Windows User’s Guide: Software for Canonical Community Ordination (version 4.5). (Microcomputer Power, 2002).

Stika, H.-P. & Heiss, A. G. in The Oxford Handbook of the European Bronze Age 348–369 (Oxford University Press, 2013).

De Ceuster, S. & Degryse, P. A ‘match–no match‘ numerical and graphical kernel density approach to interpreting lead isotope signatures of ancient artefacts. DOI