Tandem Affinity Purification [tandemová afinitní purifikace]

topical
Terms

tandemová afinitní chromatografie

 

Tandem Affinity Chromatography

Persistent link   https://www.medvik.cz/link/D000078791
Definition

A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.

DUI
D000078791 MeSH Browser
CUI
M000643237
Previous indexing
Chromatography, Affinity (2002-2018)
History note
2019
Public note
2019

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CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods
ST
standards
SN
statistics & numerical data
TD
trends
VE
veterinary

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 790
E02 Therapeutics 2 575
E03 Anesthesia and Analgesia 168
E04 Surgical Procedures, Operative 4 389
E05 Investigative Techniques 209
E05.196 Chemistry Techniques, Analytical 1 182
E05.196.181 Chromatography 834
E05.196.181.400 Chromatography, Liquid 711
E05.196.181.400.170 Chromatography, Affinity 169
E05.196.181.400.170.500 Immunochromatography obsolete
E05.196.181.400.170.750 Tandem Affinity Purification
E06 Dentistry 1 670
E07 Equipment and Supplies 4 036

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