A technique used to separate particles according to their densities in a continuous density gradient. The sample is usually mixed with a solution of known gradient materials and subjected to centrifugation. Each particle sediments to the position at which the gradient density is equal to its own. The range of the density gradient is usually greater than that of the sample particles. It is used in purifying biological materials such as proteins, nucleic acids, organelles, and cell types.

DUI

D002500 MeSH Browser

CUI

M0003812

History note

91(75); was see under CENTRIFUGATION, DENSITY GRADIENT 1975-90

Online note

search CENTRIFUGATION, DENSITY GRADIENT 1968-74

Public note

91; was see under CENTRIFUGATION, DENSITY GRADIENT 1975-90