Freeze Fracturing [mrazové lámání]

topical
4
Terms

lámání mrazem

Persistent link   https://www.medvik.cz/link/D005614
Definition

Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.

Annotation
do not confuse with FREEZE ETCHING (wherein no cryoprotectant is used)
DUI
D005614 MeSH Browser
CUI
M0008836
Previous indexing
Freeze Etching (1969-1974)
History note
75
Public note
75

Allowable subheadings

CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods 1
ST
standards
SN
statistics & numerical data
TD
trends
VE
veterinary

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 793
E01.370 Diagnostic Techniques and Procedures 3 426
E01.370.225 Clinical Laboratory Techniques 6 388
E01.370.225.500 Cytological Techniques 563
E01.370.225.500.620 Histocytological Preparation Techniques 56
E01.370.225.500.620.620 Replica Techniques 2
E01.370.225.500.620.620.150 Corrosion Casting 5
E01.370.225.500.620.620.260 Freeze Fracturing 4
E01.370.225.500.620.620.260.400 Freeze Etching 2
E01.370.225.750 Histological Techniques 1 398
E01.370.225.750.600 Histocytological Preparation Techniques 56
E01.370.225.750.600.620 Replica Techniques 2
E01.370.225.750.600.620.150 Corrosion Casting 5
E01.370.225.750.600.620.260 Freeze Fracturing 4
E01.370.225.750.600.620.260.400 Freeze Etching 2
E05 Investigative Techniques 209
E05.200 Clinical Laboratory Techniques 6 388
E05.200.500 Cytological Techniques 563
E05.200.500.620 Histocytological Preparation Techniques 56
E05.200.500.620.620 Replica Techniques 2
E05.200.500.620.620.150 Corrosion Casting 5
E05.200.500.620.620.260 Freeze Fracturing 4
E05.200.500.620.620.260.400 Freeze Etching 2
E05.200.750 Histological Techniques 1 398
E05.200.750.600 Histocytological Preparation Techniques 56
E05.200.750.600.620 Replica Techniques 2
E05.200.750.600.620.150 Corrosion Casting 5
E05.200.750.600.620.260 Freeze Fracturing 4
E05.200.750.600.620.260.400 Freeze Etching 2

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